Conditioning the microenvironment for soft tissue regeneration in a cell free scaffold

Abstract The use of cell-free scaffolds for the regeneration of clinically relevant volumes of soft tissue has been challenged, particularly in the case of synthetic biomaterials, by the difficulty of reconciling the manufacturing and biological performance requirements. Here, we investigated in viv...

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Autores principales:	Irini Gerges, Margherita Tamplenizza, Federico Martello, Stefano Koman, Giulia Chincarini, Camilla Recordati, Mariacaterina Tamplenizza, Scott Guelcher, Maurizio Crestani, Alessandro Tocchio
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/365450e7d58b49c7b92472e83fc934c3
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spelling	oai:doaj.org-article:365450e7d58b49c7b92472e83fc934c32021-12-02T16:07:02ZConditioning the microenvironment for soft tissue regeneration in a cell free scaffold10.1038/s41598-021-92732-92045-2322https://doaj.org/article/365450e7d58b49c7b92472e83fc934c32021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92732-9https://doaj.org/toc/2045-2322Abstract The use of cell-free scaffolds for the regeneration of clinically relevant volumes of soft tissue has been challenged, particularly in the case of synthetic biomaterials, by the difficulty of reconciling the manufacturing and biological performance requirements. Here, we investigated in vivo the importance of biomechanical and biochemical cues for conditioning the 3D regenerative microenvironment towards soft tissue formation. In particular, we evaluated the adipogenesis changes related to 3D mechanical properties by creating a gradient of 3D microenvironments with different stiffnesses using 3D Poly(Urethane-Ester-ether) PUEt scaffolds. Our results showed a significant increase in adipose tissue proportions while decreasing the stiffness of the 3D mechanical microenvironment. This mechanical conditioning effect was also compared with biochemical manipulation by loading extracellular matrices (ECMs) with a PPAR-γ activating molecule. Notably, results showed mechanical and biochemical conditioning equivalency in promoting adipose tissue formation in the conditions tested, suggesting that adequate mechanical signaling could be sufficient to boost adipogenesis by influencing tissue remodeling. Overall, this work could open a new avenue in the design of synthetic 3D scaffolds for microenvironment conditioning towards the regeneration of large volumes of soft and adipose tissue, with practical and direct implications in reconstructive and cosmetic surgery.Irini GergesMargherita TamplenizzaFederico MartelloStefano KomanGiulia ChincariniCamilla RecordatiMariacaterina TamplenizzaScott GuelcherMaurizio CrestaniAlessandro TocchioNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Irini Gerges Margherita Tamplenizza Federico Martello Stefano Koman Giulia Chincarini Camilla Recordati Mariacaterina Tamplenizza Scott Guelcher Maurizio Crestani Alessandro Tocchio Conditioning the microenvironment for soft tissue regeneration in a cell free scaffold
description	Abstract The use of cell-free scaffolds for the regeneration of clinically relevant volumes of soft tissue has been challenged, particularly in the case of synthetic biomaterials, by the difficulty of reconciling the manufacturing and biological performance requirements. Here, we investigated in vivo the importance of biomechanical and biochemical cues for conditioning the 3D regenerative microenvironment towards soft tissue formation. In particular, we evaluated the adipogenesis changes related to 3D mechanical properties by creating a gradient of 3D microenvironments with different stiffnesses using 3D Poly(Urethane-Ester-ether) PUEt scaffolds. Our results showed a significant increase in adipose tissue proportions while decreasing the stiffness of the 3D mechanical microenvironment. This mechanical conditioning effect was also compared with biochemical manipulation by loading extracellular matrices (ECMs) with a PPAR-γ activating molecule. Notably, results showed mechanical and biochemical conditioning equivalency in promoting adipose tissue formation in the conditions tested, suggesting that adequate mechanical signaling could be sufficient to boost adipogenesis by influencing tissue remodeling. Overall, this work could open a new avenue in the design of synthetic 3D scaffolds for microenvironment conditioning towards the regeneration of large volumes of soft and adipose tissue, with practical and direct implications in reconstructive and cosmetic surgery.
format	article
author	Irini Gerges Margherita Tamplenizza Federico Martello Stefano Koman Giulia Chincarini Camilla Recordati Mariacaterina Tamplenizza Scott Guelcher Maurizio Crestani Alessandro Tocchio
author_facet	Irini Gerges Margherita Tamplenizza Federico Martello Stefano Koman Giulia Chincarini Camilla Recordati Mariacaterina Tamplenizza Scott Guelcher Maurizio Crestani Alessandro Tocchio
author_sort	Irini Gerges
title	Conditioning the microenvironment for soft tissue regeneration in a cell free scaffold
title_short	Conditioning the microenvironment for soft tissue regeneration in a cell free scaffold
title_full	Conditioning the microenvironment for soft tissue regeneration in a cell free scaffold
title_fullStr	Conditioning the microenvironment for soft tissue regeneration in a cell free scaffold
title_full_unstemmed	Conditioning the microenvironment for soft tissue regeneration in a cell free scaffold
title_sort	conditioning the microenvironment for soft tissue regeneration in a cell free scaffold
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/365450e7d58b49c7b92472e83fc934c3
work_keys_str_mv	AT irinigerges conditioningthemicroenvironmentforsofttissueregenerationinacellfreescaffold AT margheritatamplenizza conditioningthemicroenvironmentforsofttissueregenerationinacellfreescaffold AT federicomartello conditioningthemicroenvironmentforsofttissueregenerationinacellfreescaffold AT stefanokoman conditioningthemicroenvironmentforsofttissueregenerationinacellfreescaffold AT giuliachincarini conditioningthemicroenvironmentforsofttissueregenerationinacellfreescaffold AT camillarecordati conditioningthemicroenvironmentforsofttissueregenerationinacellfreescaffold AT mariacaterinatamplenizza conditioningthemicroenvironmentforsofttissueregenerationinacellfreescaffold AT scottguelcher conditioningthemicroenvironmentforsofttissueregenerationinacellfreescaffold AT mauriziocrestani conditioningthemicroenvironmentforsofttissueregenerationinacellfreescaffold AT alessandrotocchio conditioningthemicroenvironmentforsofttissueregenerationinacellfreescaffold
_version_	1718384768864747520

Conditioning the microenvironment for soft tissue regeneration in a cell free scaffold

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