YAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition

Muscle stem cells (MuSCs) isolated ex vivo are essential original cells to produce cultured meat. Currently, one of the main obstacles for cultured meat production derives from the limited capacity of large-scale amplification of MuSCs, especially under high-density culture condition. Here, we show...

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Autores principales:	Zheng Liu, Ling Lin, Haozhe Zhu, Zhongyuan Wu, Xi Ding, Rongrong Hu, Yichen Jiang, Changbo Tang, Shijie Ding, Renpeng Guo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	muscle stem cells high-density culture Hippo-YAP cell proliferation stemness maintenance cultured meat Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3655f305a3c24ceb8f386ba6c94d5e77
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spelling	oai:doaj.org-article:3655f305a3c24ceb8f386ba6c94d5e772021-11-25T17:11:08ZYAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition10.3390/cells101130692073-4409https://doaj.org/article/3655f305a3c24ceb8f386ba6c94d5e772021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3069https://doaj.org/toc/2073-4409Muscle stem cells (MuSCs) isolated ex vivo are essential original cells to produce cultured meat. Currently, one of the main obstacles for cultured meat production derives from the limited capacity of large-scale amplification of MuSCs, especially under high-density culture condition. Here, we show that at higher cell densities, proliferation and differentiation capacities of porcine MuSCs are impaired. We investigate the roles of Hippo-YAP signaling, which is important regulators in response to cell contact inhibition. Interestingly, abundant but not functional YAP proteins are accumulated in MuSCs seeded at high density. When treated with lysophosphatidic acid (LPA), the activator of YAP, porcine MuSCs exhibit increased proliferation and elevated differentiation potential compared with control cells. Moreover, constitutively active YAP with deactivated phosphorylation sites, but not intact YAP, promotes cell proliferation and stemness maintenance of MuSCs. Together, we reveal a potential molecular target that enables massive MuSCs expansion for large-scale cultured meat production under high-density condition.Zheng LiuLing LinHaozhe ZhuZhongyuan WuXi DingRongrong HuYichen JiangChangbo TangShijie DingRenpeng GuoMDPI AGarticlemuscle stem cellshigh-density cultureHippo-YAPcell proliferationstemness maintenancecultured meatBiology (General)QH301-705.5ENCells, Vol 10, Iss 3069, p 3069 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	muscle stem cells high-density culture Hippo-YAP cell proliferation stemness maintenance cultured meat Biology (General) QH301-705.5
spellingShingle	muscle stem cells high-density culture Hippo-YAP cell proliferation stemness maintenance cultured meat Biology (General) QH301-705.5 Zheng Liu Ling Lin Haozhe Zhu Zhongyuan Wu Xi Ding Rongrong Hu Yichen Jiang Changbo Tang Shijie Ding Renpeng Guo YAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition
description	Muscle stem cells (MuSCs) isolated ex vivo are essential original cells to produce cultured meat. Currently, one of the main obstacles for cultured meat production derives from the limited capacity of large-scale amplification of MuSCs, especially under high-density culture condition. Here, we show that at higher cell densities, proliferation and differentiation capacities of porcine MuSCs are impaired. We investigate the roles of Hippo-YAP signaling, which is important regulators in response to cell contact inhibition. Interestingly, abundant but not functional YAP proteins are accumulated in MuSCs seeded at high density. When treated with lysophosphatidic acid (LPA), the activator of YAP, porcine MuSCs exhibit increased proliferation and elevated differentiation potential compared with control cells. Moreover, constitutively active YAP with deactivated phosphorylation sites, but not intact YAP, promotes cell proliferation and stemness maintenance of MuSCs. Together, we reveal a potential molecular target that enables massive MuSCs expansion for large-scale cultured meat production under high-density condition.
format	article
author	Zheng Liu Ling Lin Haozhe Zhu Zhongyuan Wu Xi Ding Rongrong Hu Yichen Jiang Changbo Tang Shijie Ding Renpeng Guo
author_facet	Zheng Liu Ling Lin Haozhe Zhu Zhongyuan Wu Xi Ding Rongrong Hu Yichen Jiang Changbo Tang Shijie Ding Renpeng Guo
author_sort	Zheng Liu
title	YAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition
title_short	YAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition
title_full	YAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition
title_fullStr	YAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition
title_full_unstemmed	YAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition
title_sort	yap promotes cell proliferation and stemness maintenance of porcine muscle stem cells under high-density condition
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3655f305a3c24ceb8f386ba6c94d5e77
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YAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition

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