Regulating endoplasmic reticulum localization of a fluorescent NBD adamantane conjugate by Cucurbit [7]uril
Intracellular localization of small molecule organic fluorescent probes by supra-molecular control is a fascinating approach. Here, we report an adamantane conjugated nitrobenzoxadiazole (NBD-Ad)-based fluorescent probe that efficiently labels the endoplasmic reticulum (ER) in living cells. Alongsid...
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| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/365d1a9fed4b459193c6b7fa3447e5d1 |
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| Sumario: | Intracellular localization of small molecule organic fluorescent probes by supra-molecular control is a fascinating approach. Here, we report an adamantane conjugated nitrobenzoxadiazole (NBD-Ad)-based fluorescent probe that efficiently labels the endoplasmic reticulum (ER) in living cells. Alongside, we have chosen cucurbit [7]uril (CB7), a water-soluble and biocompatible macrocyclic host as a supramolecular cavity having a strong micromolar binding affinity to the NBD-Ad probe. The photophysical properties of NBD-Ad and the binding studies with CB7 have been performed using various spectroscopic techniques. After confirming the ER-specific sub-cellular localization of the probe, the internalization has been studied in presence of CB7 in a time-dependent manner. The reduction in the fluorescence signal along with loss in specificity for ER with increasing CB7 treatment period highlights the controlled sub-cellular localization and transport of small molecule. |
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