Regulating endoplasmic reticulum localization of a fluorescent NBD adamantane conjugate by Cucurbit [7]uril

Intracellular localization of small molecule organic fluorescent probes by supra-molecular control is a fascinating approach. Here, we report an adamantane conjugated nitrobenzoxadiazole (NBD-Ad)-based fluorescent probe that efficiently labels the endoplasmic reticulum (ER) in living cells. Alongsid...

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Autores principales:	Tanoy Dutta, Shrobona Banerjee, Apurba Lal Koner
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Macrocyclic host Cucurbit [7]uril Drug Live-cell imaging Organelle targeting Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/365d1a9fed4b459193c6b7fa3447e5d1
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id	oai:doaj.org-article:365d1a9fed4b459193c6b7fa3447e5d1
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spelling	oai:doaj.org-article:365d1a9fed4b459193c6b7fa3447e5d12021-11-06T04:42:07ZRegulating endoplasmic reticulum localization of a fluorescent NBD adamantane conjugate by Cucurbit [7]uril2667-022410.1016/j.chphi.2021.100051https://doaj.org/article/365d1a9fed4b459193c6b7fa3447e5d12021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2667022421000402https://doaj.org/toc/2667-0224Intracellular localization of small molecule organic fluorescent probes by supra-molecular control is a fascinating approach. Here, we report an adamantane conjugated nitrobenzoxadiazole (NBD-Ad)-based fluorescent probe that efficiently labels the endoplasmic reticulum (ER) in living cells. Alongside, we have chosen cucurbit [7]uril (CB7), a water-soluble and biocompatible macrocyclic host as a supramolecular cavity having a strong micromolar binding affinity to the NBD-Ad probe. The photophysical properties of NBD-Ad and the binding studies with CB7 have been performed using various spectroscopic techniques. After confirming the ER-specific sub-cellular localization of the probe, the internalization has been studied in presence of CB7 in a time-dependent manner. The reduction in the fluorescence signal along with loss in specificity for ER with increasing CB7 treatment period highlights the controlled sub-cellular localization and transport of small molecule.Tanoy DuttaShrobona BanerjeeApurba Lal KonerElsevierarticleMacrocyclic hostCucurbit [7]urilDrugLive-cell imagingOrganelle targetingPhysicsQC1-999ChemistryQD1-999ENChemical Physics Impact, Vol 3, Iss , Pp 100051- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Macrocyclic host Cucurbit [7]uril Drug Live-cell imaging Organelle targeting Physics QC1-999 Chemistry QD1-999
spellingShingle	Macrocyclic host Cucurbit [7]uril Drug Live-cell imaging Organelle targeting Physics QC1-999 Chemistry QD1-999 Tanoy Dutta Shrobona Banerjee Apurba Lal Koner Regulating endoplasmic reticulum localization of a fluorescent NBD adamantane conjugate by Cucurbit [7]uril
description	Intracellular localization of small molecule organic fluorescent probes by supra-molecular control is a fascinating approach. Here, we report an adamantane conjugated nitrobenzoxadiazole (NBD-Ad)-based fluorescent probe that efficiently labels the endoplasmic reticulum (ER) in living cells. Alongside, we have chosen cucurbit [7]uril (CB7), a water-soluble and biocompatible macrocyclic host as a supramolecular cavity having a strong micromolar binding affinity to the NBD-Ad probe. The photophysical properties of NBD-Ad and the binding studies with CB7 have been performed using various spectroscopic techniques. After confirming the ER-specific sub-cellular localization of the probe, the internalization has been studied in presence of CB7 in a time-dependent manner. The reduction in the fluorescence signal along with loss in specificity for ER with increasing CB7 treatment period highlights the controlled sub-cellular localization and transport of small molecule.
format	article
author	Tanoy Dutta Shrobona Banerjee Apurba Lal Koner
author_facet	Tanoy Dutta Shrobona Banerjee Apurba Lal Koner
author_sort	Tanoy Dutta
title	Regulating endoplasmic reticulum localization of a fluorescent NBD adamantane conjugate by Cucurbit [7]uril
title_short	Regulating endoplasmic reticulum localization of a fluorescent NBD adamantane conjugate by Cucurbit [7]uril
title_full	Regulating endoplasmic reticulum localization of a fluorescent NBD adamantane conjugate by Cucurbit [7]uril
title_fullStr	Regulating endoplasmic reticulum localization of a fluorescent NBD adamantane conjugate by Cucurbit [7]uril
title_full_unstemmed	Regulating endoplasmic reticulum localization of a fluorescent NBD adamantane conjugate by Cucurbit [7]uril
title_sort	regulating endoplasmic reticulum localization of a fluorescent nbd adamantane conjugate by cucurbit [7]uril
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/365d1a9fed4b459193c6b7fa3447e5d1
work_keys_str_mv	AT tanoydutta regulatingendoplasmicreticulumlocalizationofafluorescentnbdadamantaneconjugatebycucurbit7uril AT shrobonabanerjee regulatingendoplasmicreticulumlocalizationofafluorescentnbdadamantaneconjugatebycucurbit7uril AT apurbalalkoner regulatingendoplasmicreticulumlocalizationofafluorescentnbdadamantaneconjugatebycucurbit7uril
_version_	1718443845924945920

Regulating endoplasmic reticulum localization of a fluorescent NBD adamantane conjugate by Cucurbit [7]uril

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