Evaluation of circulating miRNAs and mRNAs expression patterns in autism spectrum disorder

Abstract Background Autism spectrum disorder is a condition related to brain development that affects a person’s perception and socialization, resulting in problems in social interaction and communication. It has no single known cause, yet several different genes appear to be involved in autism. As...

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Autores principales:	Amany H. Abdelrahman, Ola M. Eid, Mona H. Ibrahim, Safa N. Abd El-Fattah, Maha M. Eid, Nagwa A. Meguid
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	ASD miR128 miR7 SHANK family Medicine (General) R5-920 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/365daf5ec31448868cc9d888d4f31d50
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spelling	oai:doaj.org-article:365daf5ec31448868cc9d888d4f31d502021-11-21T12:03:55ZEvaluation of circulating miRNAs and mRNAs expression patterns in autism spectrum disorder10.1186/s43042-021-00202-82090-2441https://doaj.org/article/365daf5ec31448868cc9d888d4f31d502021-11-01T00:00:00Zhttps://doi.org/10.1186/s43042-021-00202-8https://doaj.org/toc/2090-2441Abstract Background Autism spectrum disorder is a condition related to brain development that affects a person’s perception and socialization, resulting in problems in social interaction and communication. It has no single known cause, yet several different genes appear to be involved in autism. As a genetically complex disease, dysregulation of miRNA expression and miRNA–mRNA interactions might be a feature of autism spectrum disorder. The aim of the current study was to investigate the expression profile of circulating miRNA-128, miRNA-7 and SHANK gene family in ASD patients and to assess the possible influence of miRNA-128 and miRNA-7 on SHANK genes, which might provide an insight into the pathogenic mechanisms of ASD and introduce noninvasive molecular biomarkers for the disease diagnosis and prognosis. Quantitative real-time PCR technique was employed to determine expression levels of miRNA-128, miRNA-7 and SHANK gene family in blood samples of 40 autistic cases along with 30 age- and sex-matched normal volunteer subjects. Results Our study revealed a statistical significant upregulation of miRNA-128 expression levels in ASD cases compared to controls (p value < 0.001). A statistical significant difference in SHANK-3 expression was encountered on comparing cases to controls (p value < 0.001). However, miRNA-7 expression showed no significant difference between the studied groups. Conclusions MiRNA-128 and SHANK-3 gene are emerging players in the field of ASD. They are promising candidates as noninvasive biomarkers in autism. Future studies are needed to emphasize their pivotal role.Amany H. AbdelrahmanOla M. EidMona H. IbrahimSafa N. Abd El-FattahMaha M. EidNagwa A. MeguidSpringerOpenarticleASDmiR128miR7SHANK familyMedicine (General)R5-920GeneticsQH426-470ENEgyptian Journal of Medical Human Genetics, Vol 22, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ASD miR128 miR7 SHANK family Medicine (General) R5-920 Genetics QH426-470
spellingShingle	ASD miR128 miR7 SHANK family Medicine (General) R5-920 Genetics QH426-470 Amany H. Abdelrahman Ola M. Eid Mona H. Ibrahim Safa N. Abd El-Fattah Maha M. Eid Nagwa A. Meguid Evaluation of circulating miRNAs and mRNAs expression patterns in autism spectrum disorder
description	Abstract Background Autism spectrum disorder is a condition related to brain development that affects a person’s perception and socialization, resulting in problems in social interaction and communication. It has no single known cause, yet several different genes appear to be involved in autism. As a genetically complex disease, dysregulation of miRNA expression and miRNA–mRNA interactions might be a feature of autism spectrum disorder. The aim of the current study was to investigate the expression profile of circulating miRNA-128, miRNA-7 and SHANK gene family in ASD patients and to assess the possible influence of miRNA-128 and miRNA-7 on SHANK genes, which might provide an insight into the pathogenic mechanisms of ASD and introduce noninvasive molecular biomarkers for the disease diagnosis and prognosis. Quantitative real-time PCR technique was employed to determine expression levels of miRNA-128, miRNA-7 and SHANK gene family in blood samples of 40 autistic cases along with 30 age- and sex-matched normal volunteer subjects. Results Our study revealed a statistical significant upregulation of miRNA-128 expression levels in ASD cases compared to controls (p value < 0.001). A statistical significant difference in SHANK-3 expression was encountered on comparing cases to controls (p value < 0.001). However, miRNA-7 expression showed no significant difference between the studied groups. Conclusions MiRNA-128 and SHANK-3 gene are emerging players in the field of ASD. They are promising candidates as noninvasive biomarkers in autism. Future studies are needed to emphasize their pivotal role.
format	article
author	Amany H. Abdelrahman Ola M. Eid Mona H. Ibrahim Safa N. Abd El-Fattah Maha M. Eid Nagwa A. Meguid
author_facet	Amany H. Abdelrahman Ola M. Eid Mona H. Ibrahim Safa N. Abd El-Fattah Maha M. Eid Nagwa A. Meguid
author_sort	Amany H. Abdelrahman
title	Evaluation of circulating miRNAs and mRNAs expression patterns in autism spectrum disorder
title_short	Evaluation of circulating miRNAs and mRNAs expression patterns in autism spectrum disorder
title_full	Evaluation of circulating miRNAs and mRNAs expression patterns in autism spectrum disorder
title_fullStr	Evaluation of circulating miRNAs and mRNAs expression patterns in autism spectrum disorder
title_full_unstemmed	Evaluation of circulating miRNAs and mRNAs expression patterns in autism spectrum disorder
title_sort	evaluation of circulating mirnas and mrnas expression patterns in autism spectrum disorder
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/365daf5ec31448868cc9d888d4f31d50
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Evaluation of circulating miRNAs and mRNAs expression patterns in autism spectrum disorder

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