Global genotype-phenotype correlations in Pseudomonas aeruginosa.

Once the genome sequence of an organism is obtained, attention turns from identifying genes to understanding their function, their organization and control of metabolic pathways and networks that determine its physiology. Recent technical advances in acquiring genome-wide data have led to substantia...

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Autores principales:	Claudia Pommerenke, Mathias Müsken, Tanja Becker, Andreas Dötsch, Frank Klawonn, Susanne Häussler
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/36610b350f4840dc805f079061e1db26
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spelling	oai:doaj.org-article:36610b350f4840dc805f079061e1db262021-11-18T06:01:41ZGlobal genotype-phenotype correlations in Pseudomonas aeruginosa.1553-73661553-737410.1371/journal.ppat.1001074https://doaj.org/article/36610b350f4840dc805f079061e1db262010-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20865161/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Once the genome sequence of an organism is obtained, attention turns from identifying genes to understanding their function, their organization and control of metabolic pathways and networks that determine its physiology. Recent technical advances in acquiring genome-wide data have led to substantial progress in identifying gene functions. However, we still do not know the function of a large number of genes and, even when a gene product has been assigned to a functional class, we cannot normally predict its contribution to the phenotypic behaviour of the cell or organism--the phenome. In this study, we assessed bacterial growth parameters of 4030 non-redundant PA14 transposon mutants in the pathogenic bacterium Pseudomonas aeruginosa. The genome-wide simultaneous analysis of 119 distinct growth-related phenotypes uncovered a comprehensive phenome and provided evidence that most genotypes are not phenotypically isolated but rather define specific complex phenotypic clusters of genotypes. Since phenotypic overlap was demonstrated to reflect the relatedness of genotypes on a global scale, knowledge of an organism's phenome might significantly contribute to the advancement of functional genomics.Claudia PommerenkeMathias MüskenTanja BeckerAndreas DötschFrank KlawonnSusanne HäusslerPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 6, Iss 8, p e1001074 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Claudia Pommerenke Mathias Müsken Tanja Becker Andreas Dötsch Frank Klawonn Susanne Häussler Global genotype-phenotype correlations in Pseudomonas aeruginosa.
description	Once the genome sequence of an organism is obtained, attention turns from identifying genes to understanding their function, their organization and control of metabolic pathways and networks that determine its physiology. Recent technical advances in acquiring genome-wide data have led to substantial progress in identifying gene functions. However, we still do not know the function of a large number of genes and, even when a gene product has been assigned to a functional class, we cannot normally predict its contribution to the phenotypic behaviour of the cell or organism--the phenome. In this study, we assessed bacterial growth parameters of 4030 non-redundant PA14 transposon mutants in the pathogenic bacterium Pseudomonas aeruginosa. The genome-wide simultaneous analysis of 119 distinct growth-related phenotypes uncovered a comprehensive phenome and provided evidence that most genotypes are not phenotypically isolated but rather define specific complex phenotypic clusters of genotypes. Since phenotypic overlap was demonstrated to reflect the relatedness of genotypes on a global scale, knowledge of an organism's phenome might significantly contribute to the advancement of functional genomics.
format	article
author	Claudia Pommerenke Mathias Müsken Tanja Becker Andreas Dötsch Frank Klawonn Susanne Häussler
author_facet	Claudia Pommerenke Mathias Müsken Tanja Becker Andreas Dötsch Frank Klawonn Susanne Häussler
author_sort	Claudia Pommerenke
title	Global genotype-phenotype correlations in Pseudomonas aeruginosa.
title_short	Global genotype-phenotype correlations in Pseudomonas aeruginosa.
title_full	Global genotype-phenotype correlations in Pseudomonas aeruginosa.
title_fullStr	Global genotype-phenotype correlations in Pseudomonas aeruginosa.
title_full_unstemmed	Global genotype-phenotype correlations in Pseudomonas aeruginosa.
title_sort	global genotype-phenotype correlations in pseudomonas aeruginosa.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/36610b350f4840dc805f079061e1db26
work_keys_str_mv	AT claudiapommerenke globalgenotypephenotypecorrelationsinpseudomonasaeruginosa AT mathiasmusken globalgenotypephenotypecorrelationsinpseudomonasaeruginosa AT tanjabecker globalgenotypephenotypecorrelationsinpseudomonasaeruginosa AT andreasdotsch globalgenotypephenotypecorrelationsinpseudomonasaeruginosa AT frankklawonn globalgenotypephenotypecorrelationsinpseudomonasaeruginosa AT susannehaussler globalgenotypephenotypecorrelationsinpseudomonasaeruginosa
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Global genotype-phenotype correlations in Pseudomonas aeruginosa.

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