Molecular Characterization of ESBL-Producing Escherichia Coli Isolated from Healthy Cattle and Sheep

The present study aims to characterize ESBL-producing Escherichia coli isolated from healthy cattle and sheep in the Burdur province of Turkey. Fecal samples from a total of 200 cattle and 200 sheep were tested and ESBL-producing E. coli was isolated from 31 (15.5%) cattle and three (1.5%) sheep sam...

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Autores principales:	Pehlivanoglu Faruk, Turutoglu Hulya, Ozturk Dilek, Yardimci Hakan
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2016
Materias:	cattle esbl escherichia coli multidrug-resistance sheep Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/36648c895b1e488992513a333f5da483
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spelling	oai:doaj.org-article:36648c895b1e488992513a333f5da4832021-11-17T21:27:51ZMolecular Characterization of ESBL-Producing Escherichia Coli Isolated from Healthy Cattle and Sheep1820-744810.1515/acve-2016-0045https://doaj.org/article/36648c895b1e488992513a333f5da4832016-12-01T00:00:00Zhttps://doi.org/10.1515/acve-2016-0045https://doaj.org/toc/1820-7448The present study aims to characterize ESBL-producing Escherichia coli isolated from healthy cattle and sheep in the Burdur province of Turkey. Fecal samples from a total of 200 cattle and 200 sheep were tested and ESBL-producing E. coli was isolated from 31 (15.5%) cattle and three (1.5%) sheep samples using the Clinical and Laboratory Standards Institute’s combined disk method. Among the ESBL gene classes detected by PCR, blaCTX-M was the most frequent type, followed by the blaTEM and blaSHV families. ESBL-producing E. coli isolates showed co-resistance to multiple classes of antibiotics including aminoglycosides, phenicols, quinolones, folate pathway inhibitors and tetracyclines. The resistance rates were higher in the cattle isolates than in the sheep isolates. Phylogenetic grouping of the E. coli isolates indicated group A (particularly A1) was the predominant phylogenetic group (19/34, 55.9%), followed by groups B1 (9/34, 26.5%) and D (6/34, 17.6%); none of the isolates belonged to group B2. The study shows that ESBL-producing E. coli isolates exist in the intestinal flora of healthy cattle and sheep in the Burdur province of Turkey. This is the first report showing the emergence of CTX-M type ESBL-producing E. coli in sheep farms in TurkeyPehlivanoglu FarukTurutoglu HulyaOzturk DilekYardimci HakanSciendoarticlecattleesblescherichia colimultidrug-resistancesheepVeterinary medicineSF600-1100ENActa Veterinaria, Vol 66, Iss 4, Pp 520-533 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	cattle esbl escherichia coli multidrug-resistance sheep Veterinary medicine SF600-1100
spellingShingle	cattle esbl escherichia coli multidrug-resistance sheep Veterinary medicine SF600-1100 Pehlivanoglu Faruk Turutoglu Hulya Ozturk Dilek Yardimci Hakan Molecular Characterization of ESBL-Producing Escherichia Coli Isolated from Healthy Cattle and Sheep
description	The present study aims to characterize ESBL-producing Escherichia coli isolated from healthy cattle and sheep in the Burdur province of Turkey. Fecal samples from a total of 200 cattle and 200 sheep were tested and ESBL-producing E. coli was isolated from 31 (15.5%) cattle and three (1.5%) sheep samples using the Clinical and Laboratory Standards Institute’s combined disk method. Among the ESBL gene classes detected by PCR, blaCTX-M was the most frequent type, followed by the blaTEM and blaSHV families. ESBL-producing E. coli isolates showed co-resistance to multiple classes of antibiotics including aminoglycosides, phenicols, quinolones, folate pathway inhibitors and tetracyclines. The resistance rates were higher in the cattle isolates than in the sheep isolates. Phylogenetic grouping of the E. coli isolates indicated group A (particularly A1) was the predominant phylogenetic group (19/34, 55.9%), followed by groups B1 (9/34, 26.5%) and D (6/34, 17.6%); none of the isolates belonged to group B2. The study shows that ESBL-producing E. coli isolates exist in the intestinal flora of healthy cattle and sheep in the Burdur province of Turkey. This is the first report showing the emergence of CTX-M type ESBL-producing E. coli in sheep farms in Turkey
format	article
author	Pehlivanoglu Faruk Turutoglu Hulya Ozturk Dilek Yardimci Hakan
author_facet	Pehlivanoglu Faruk Turutoglu Hulya Ozturk Dilek Yardimci Hakan
author_sort	Pehlivanoglu Faruk
title	Molecular Characterization of ESBL-Producing Escherichia Coli Isolated from Healthy Cattle and Sheep
title_short	Molecular Characterization of ESBL-Producing Escherichia Coli Isolated from Healthy Cattle and Sheep
title_full	Molecular Characterization of ESBL-Producing Escherichia Coli Isolated from Healthy Cattle and Sheep
title_fullStr	Molecular Characterization of ESBL-Producing Escherichia Coli Isolated from Healthy Cattle and Sheep
title_full_unstemmed	Molecular Characterization of ESBL-Producing Escherichia Coli Isolated from Healthy Cattle and Sheep
title_sort	molecular characterization of esbl-producing escherichia coli isolated from healthy cattle and sheep
publisher	Sciendo
publishDate	2016
url	https://doaj.org/article/36648c895b1e488992513a333f5da483
work_keys_str_mv	AT pehlivanoglufaruk molecularcharacterizationofesblproducingescherichiacoliisolatedfromhealthycattleandsheep AT turutogluhulya molecularcharacterizationofesblproducingescherichiacoliisolatedfromhealthycattleandsheep AT ozturkdilek molecularcharacterizationofesblproducingescherichiacoliisolatedfromhealthycattleandsheep AT yardimcihakan molecularcharacterizationofesblproducingescherichiacoliisolatedfromhealthycattleandsheep
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Molecular Characterization of ESBL-Producing Escherichia Coli Isolated from Healthy Cattle and Sheep

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