Nuclear DNA markers for identification of Beluga and Sterlet sturgeons and their interspecific Bester hybrid

Abstract Sturgeons (Acipenseriformes) are among the most endangered species in the world due to fragmentation and destruction of their natural habitats and to overexploitation, mainly for highly priced caviar. This has led to the development of sturgeon culture, originally for reintroduction, but mo...

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Autores principales:	Miloš Havelka, Takafumi Fujimoto, Seishi Hagihara, Shinji Adachi, Katsutoshi Arai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3669ad7eee0f4a9aafc0d0e5046ab31e
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spelling	oai:doaj.org-article:3669ad7eee0f4a9aafc0d0e5046ab31e2021-12-02T12:32:27ZNuclear DNA markers for identification of Beluga and Sterlet sturgeons and their interspecific Bester hybrid10.1038/s41598-017-01768-32045-2322https://doaj.org/article/3669ad7eee0f4a9aafc0d0e5046ab31e2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01768-3https://doaj.org/toc/2045-2322Abstract Sturgeons (Acipenseriformes) are among the most endangered species in the world due to fragmentation and destruction of their natural habitats and to overexploitation, mainly for highly priced caviar. This has led to the development of sturgeon culture, originally for reintroduction, but more recently for caviar production. In both cases, accurate species identification is essential. We report a new tool for accurate identification of Huso huso and Acipenser ruthenus based on nuclear DNA markers. We employed ddRAD sequencing to identify species-specific nucleotide variants, which served as specific binding sites for diagnostic primers. The primers allowed identification of Huso huso and Acipenser ruthenus as well as their discrimination from A. baerii, A. schrenckii, A. gueldenstaedtii, A. stellatus, A. persicus, A. mikadoi, A. transmontanus, and H. dauricus and identification of A. ruthenus and H. huso hybrids with these species, except hybrid between A. ruthenus and A. stellatus. The species-specific primers also allowed identification of bester (H. huso × A. ruthenus), the most commercially exploited sturgeon hybrid. The tool, based on simple PCR and gel electrophoresis, is rapid, inexpensive, and reproducible. It will contribute to conservation of remaining wild populations of A. ruthenus and H. huso, as well as to traceability of their products.Miloš HavelkaTakafumi FujimotoSeishi HagiharaShinji AdachiKatsutoshi AraiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Miloš Havelka Takafumi Fujimoto Seishi Hagihara Shinji Adachi Katsutoshi Arai Nuclear DNA markers for identification of Beluga and Sterlet sturgeons and their interspecific Bester hybrid
description	Abstract Sturgeons (Acipenseriformes) are among the most endangered species in the world due to fragmentation and destruction of their natural habitats and to overexploitation, mainly for highly priced caviar. This has led to the development of sturgeon culture, originally for reintroduction, but more recently for caviar production. In both cases, accurate species identification is essential. We report a new tool for accurate identification of Huso huso and Acipenser ruthenus based on nuclear DNA markers. We employed ddRAD sequencing to identify species-specific nucleotide variants, which served as specific binding sites for diagnostic primers. The primers allowed identification of Huso huso and Acipenser ruthenus as well as their discrimination from A. baerii, A. schrenckii, A. gueldenstaedtii, A. stellatus, A. persicus, A. mikadoi, A. transmontanus, and H. dauricus and identification of A. ruthenus and H. huso hybrids with these species, except hybrid between A. ruthenus and A. stellatus. The species-specific primers also allowed identification of bester (H. huso × A. ruthenus), the most commercially exploited sturgeon hybrid. The tool, based on simple PCR and gel electrophoresis, is rapid, inexpensive, and reproducible. It will contribute to conservation of remaining wild populations of A. ruthenus and H. huso, as well as to traceability of their products.
format	article
author	Miloš Havelka Takafumi Fujimoto Seishi Hagihara Shinji Adachi Katsutoshi Arai
author_facet	Miloš Havelka Takafumi Fujimoto Seishi Hagihara Shinji Adachi Katsutoshi Arai
author_sort	Miloš Havelka
title	Nuclear DNA markers for identification of Beluga and Sterlet sturgeons and their interspecific Bester hybrid
title_short	Nuclear DNA markers for identification of Beluga and Sterlet sturgeons and their interspecific Bester hybrid
title_full	Nuclear DNA markers for identification of Beluga and Sterlet sturgeons and their interspecific Bester hybrid
title_fullStr	Nuclear DNA markers for identification of Beluga and Sterlet sturgeons and their interspecific Bester hybrid
title_full_unstemmed	Nuclear DNA markers for identification of Beluga and Sterlet sturgeons and their interspecific Bester hybrid
title_sort	nuclear dna markers for identification of beluga and sterlet sturgeons and their interspecific bester hybrid
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3669ad7eee0f4a9aafc0d0e5046ab31e
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Nuclear DNA markers for identification of Beluga and Sterlet sturgeons and their interspecific Bester hybrid

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