A knockout cell library of GPI biosynthetic genes for functional studies of GPI-anchored proteins

A knockout cell library of GPI biosynthetic genes for functional studies of GPI-anchored proteins

Liu et al. construct a knock out cell library of genes involved in the biosynthesis of glycosylphosphatidylinositol (GPI)-anchored protein (GPI-APs) from HEK-293 cells and analyzed the surface expression and sensitivity to phosphatidylinositol-specific phospholipase C. Furthermore, the authors also...

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Autores principales: Si-Si Liu, Yi-Shi Liu, Xin-Yu Guo, Yoshiko Murakami, Ganglong Yang, Xiao-Dong Gao, Taroh Kinoshita, Morihisa Fujita
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Biology (General)
QH301-705.5
Acceso en línea:https://doaj.org/article/3671a2fc33684958a4cc3730bc6d41d1
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spelling oai:doaj.org-article:3671a2fc33684958a4cc3730bc6d41d12021-12-02T16:05:53ZA knockout cell library of GPI biosynthetic genes for functional studies of GPI-anchored proteins10.1038/s42003-021-02337-12399-3642https://doaj.org/article/3671a2fc33684958a4cc3730bc6d41d12021-06-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02337-1https://doaj.org/toc/2399-3642Liu et al. construct a knock out cell library of genes involved in the biosynthesis of glycosylphosphatidylinositol (GPI)-anchored protein (GPI-APs) from HEK-293 cells and analyzed the surface expression and sensitivity to phosphatidylinositol-specific phospholipase C. Furthermore, the authors also identify structural motifs of GPIs that are recognized by a GPI-binding toxin, aerolysin.Si-Si LiuYi-Shi LiuXin-Yu GuoYoshiko MurakamiGanglong YangXiao-Dong GaoTaroh KinoshitaMorihisa FujitaNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Biology (General)
QH301-705.5
spellingShingle Biology (General)
QH301-705.5
Si-Si Liu
Yi-Shi Liu
Xin-Yu Guo
Yoshiko Murakami
Ganglong Yang
Xiao-Dong Gao
Taroh Kinoshita
Morihisa Fujita
A knockout cell library of GPI biosynthetic genes for functional studies of GPI-anchored proteins
description Liu et al. construct a knock out cell library of genes involved in the biosynthesis of glycosylphosphatidylinositol (GPI)-anchored protein (GPI-APs) from HEK-293 cells and analyzed the surface expression and sensitivity to phosphatidylinositol-specific phospholipase C. Furthermore, the authors also identify structural motifs of GPIs that are recognized by a GPI-binding toxin, aerolysin.
format article
author Si-Si Liu
Yi-Shi Liu
Xin-Yu Guo
Yoshiko Murakami
Ganglong Yang
Xiao-Dong Gao
Taroh Kinoshita
Morihisa Fujita
author_facet Si-Si Liu
Yi-Shi Liu
Xin-Yu Guo
Yoshiko Murakami
Ganglong Yang
Xiao-Dong Gao
Taroh Kinoshita
Morihisa Fujita
author_sort Si-Si Liu
title A knockout cell library of GPI biosynthetic genes for functional studies of GPI-anchored proteins
title_short A knockout cell library of GPI biosynthetic genes for functional studies of GPI-anchored proteins
title_full A knockout cell library of GPI biosynthetic genes for functional studies of GPI-anchored proteins
title_fullStr A knockout cell library of GPI biosynthetic genes for functional studies of GPI-anchored proteins
title_full_unstemmed A knockout cell library of GPI biosynthetic genes for functional studies of GPI-anchored proteins
title_sort knockout cell library of gpi biosynthetic genes for functional studies of gpi-anchored proteins
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/3671a2fc33684958a4cc3730bc6d41d1
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