Preparation of brushite cements with improved properties by adding graphene oxide

Negar Nasrollahi,1 Azar Nourian Dehkordi,1 Abbas Jamshidizad,1 Mohammad Chehelgerdi21Department of Stem Cell and Regenerative Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran; 2Biotechnology Research Center, Shahrekord Branch, Is...

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Autores principales:	Nasrollahi N, Nourian Dehkordi A, Jamshidizad A, Chehelgerdi M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Calcium phosphate cement graphene oxide biocompatibility bioactivity mechanical properties Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/36720208d81b488da065dd0bd1766cf7
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spelling	oai:doaj.org-article:36720208d81b488da065dd0bd1766cf72021-12-02T07:32:15ZPreparation of brushite cements with improved properties by adding graphene oxide1178-2013https://doaj.org/article/36720208d81b488da065dd0bd1766cf72019-05-01T00:00:00Zhttps://www.dovepress.com/preparation-of-brushite-cements-with-improved-properties-by-adding-gra-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Negar Nasrollahi,1 Azar Nourian Dehkordi,1 Abbas Jamshidizad,1 Mohammad Chehelgerdi21Department of Stem Cell and Regenerative Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran; 2Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, IranBackground: Brushite (dicalcium phosphate dihydrate, DCPD) cement as a promising bioactive material for bone tissue engineering is widely used to treat defects. However, relatively poor mechanical properties of brushite cement limit its application in loadbearing conditions. The aim of this study is to investigate the effect of graphene oxide (GO) addition to the physical-mechanical-biological properties of brushite cement.Methods: The brushite types of cement were prepared by mixing β-tricalcium phosphate [β-TCP, Ca3 (PO4)2] and monocalcium phosphate monohydrate [MCPM, Ca(H2PO4)2. H2O]. GO was introduced at 0, 0.5, 2, and 5 wt.% with the liquid. MG63 cells were cultured on the GO/CPC surfaces to observe various cellular activities and hydroxyapatite (HA) mineralization.Results: Based on our results, GO/CPC composites exhibit improvement in compressive strength compared to pure CPC. New Ca-deficient apatite layer was deposited on the composite surface after immersing immersion in SBF for 7 and 14 days. Field emission scanning electron microscope (FESEM) images indicated that pure and GO incorporated brushite cement facilitates cell adhesion. CPC/GO was slightly toxic to cells such that high concentrations of GO decreased the cell viability. Besides, alkaline phosphatase (ALP) activity of cells was improved compared with the pure CPC.Conclusion: Our results highlight the role of graphene oxide that may have great potential in enabling the utility of graphene-based materials in various biomedical applications.Keywords: calcium phosphate cement, graphene oxide, biocompatibility, bioactivity, mechanical propertiesNasrollahi NNourian Dehkordi AJamshidizad AChehelgerdi MDove Medical PressarticleCalcium phosphate cementgraphene oxidebiocompatibilitybioactivitymechanical propertiesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 3785-3797 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Calcium phosphate cement graphene oxide biocompatibility bioactivity mechanical properties Medicine (General) R5-920
spellingShingle	Calcium phosphate cement graphene oxide biocompatibility bioactivity mechanical properties Medicine (General) R5-920 Nasrollahi N Nourian Dehkordi A Jamshidizad A Chehelgerdi M Preparation of brushite cements with improved properties by adding graphene oxide
description	Negar Nasrollahi,1 Azar Nourian Dehkordi,1 Abbas Jamshidizad,1 Mohammad Chehelgerdi21Department of Stem Cell and Regenerative Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran; 2Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, IranBackground: Brushite (dicalcium phosphate dihydrate, DCPD) cement as a promising bioactive material for bone tissue engineering is widely used to treat defects. However, relatively poor mechanical properties of brushite cement limit its application in loadbearing conditions. The aim of this study is to investigate the effect of graphene oxide (GO) addition to the physical-mechanical-biological properties of brushite cement.Methods: The brushite types of cement were prepared by mixing β-tricalcium phosphate [β-TCP, Ca3 (PO4)2] and monocalcium phosphate monohydrate [MCPM, Ca(H2PO4)2. H2O]. GO was introduced at 0, 0.5, 2, and 5 wt.% with the liquid. MG63 cells were cultured on the GO/CPC surfaces to observe various cellular activities and hydroxyapatite (HA) mineralization.Results: Based on our results, GO/CPC composites exhibit improvement in compressive strength compared to pure CPC. New Ca-deficient apatite layer was deposited on the composite surface after immersing immersion in SBF for 7 and 14 days. Field emission scanning electron microscope (FESEM) images indicated that pure and GO incorporated brushite cement facilitates cell adhesion. CPC/GO was slightly toxic to cells such that high concentrations of GO decreased the cell viability. Besides, alkaline phosphatase (ALP) activity of cells was improved compared with the pure CPC.Conclusion: Our results highlight the role of graphene oxide that may have great potential in enabling the utility of graphene-based materials in various biomedical applications.Keywords: calcium phosphate cement, graphene oxide, biocompatibility, bioactivity, mechanical properties
format	article
author	Nasrollahi N Nourian Dehkordi A Jamshidizad A Chehelgerdi M
author_facet	Nasrollahi N Nourian Dehkordi A Jamshidizad A Chehelgerdi M
author_sort	Nasrollahi N
title	Preparation of brushite cements with improved properties by adding graphene oxide
title_short	Preparation of brushite cements with improved properties by adding graphene oxide
title_full	Preparation of brushite cements with improved properties by adding graphene oxide
title_fullStr	Preparation of brushite cements with improved properties by adding graphene oxide
title_full_unstemmed	Preparation of brushite cements with improved properties by adding graphene oxide
title_sort	preparation of brushite cements with improved properties by adding graphene oxide
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/36720208d81b488da065dd0bd1766cf7
work_keys_str_mv	AT nasrollahin preparationofbrushitecementswithimprovedpropertiesbyaddinggrapheneoxide AT nouriandehkordia preparationofbrushitecementswithimprovedpropertiesbyaddinggrapheneoxide AT jamshidizada preparationofbrushitecementswithimprovedpropertiesbyaddinggrapheneoxide AT chehelgerdim preparationofbrushitecementswithimprovedpropertiesbyaddinggrapheneoxide
_version_	1718399329322926080

Preparation of brushite cements with improved properties by adding graphene oxide

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