Evaluation of antioxidant, antimicrobial and antiproliferative activity of silver nanoparticles derived from Galphimia glauca leaf extract

Objective: The bio-synthesis of silver nanoparticles (AgNPs) is regarded as environment friendly and cost effective method compared to physical and chemical synthesis methods. Silver (Ag) is an important noble metal due to its tremendous use in research and medical fields throughout the world. This...

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Autores principales:	Bidhayak Chakraborty, Raju Suresh Kumar, Abdulrahman I. Almansour, D. Kotresha, Muthuraj Rudrappa, S.S. Pallavi, Halaswamy Hiremath, Karthikeyan Perumal, Sreenivasa Nayaka
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Galphimia glauca Green synthesis Antioxidant Antimicrobial Anticancer SK-HEP1 cell line Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/367a482a15cc427eb9b6074a0bc9e1b5
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spelling	oai:doaj.org-article:367a482a15cc427eb9b6074a0bc9e1b52021-11-18T04:44:29ZEvaluation of antioxidant, antimicrobial and antiproliferative activity of silver nanoparticles derived from Galphimia glauca leaf extract1018-364710.1016/j.jksus.2021.101660https://doaj.org/article/367a482a15cc427eb9b6074a0bc9e1b52021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1018364721003220https://doaj.org/toc/1018-3647Objective: The bio-synthesis of silver nanoparticles (AgNPs) is regarded as environment friendly and cost effective method compared to physical and chemical synthesis methods. Silver (Ag) is an important noble metal due to its tremendous use in research and medical fields throughout the world. This research work was aimed at green synthesis and characterizations of AgNPs from G. glauca leaf extract and evaluation of their bioactive potential. Methods: In the current research work, synthesis of AgNPs from G. glauca aqueous leaf extracts was performed and was characterized by UV–Visible (UV–Vis.), Fourier transform infrared (FTIR), Atomic force microscopy (AFM), Scanning electron microscopy (SEM), Energy dispersive spectroscopy (EDS), High-resolution transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), Thermo gravimetric analysis (TGA) and Zeta potential exploration. Later, antioxidant, antimicrobial activity was examined against various pathogenic organisms and anticancer activities of AgNPs were carried out against SK-HEP1 cell line. Results: The first indication of synthesis of nanoparticles was the change in color from yellowish to brown. UV–Vis. band exhibited surface plasmon resonance (SPR) at 402 nm. FTIR analysis revealed the probable bio-molecules including alkanes, alkenes, aromatics, aromatic phosphates, imine or oxime, etc. subjected to reduction of silver ions to metallic silver. The AFM, SEM and TEM analysis reported the particles were spherical shaped, poly-dispersed, 17 to 40 nm in size. The zeta potential analysis expressed a peak at –32.0 ± 0.5 mV and suggested the particles with significant long term stability. The TGA analysis revealed the stability of AgNPs at high temperature (179 ℃). The antioxidant assay of AgNPs unveiled an effective dose dependent increase in scavenging activity. Antimicrobial activity showed efficient inhibitory activity against P. aeruginosa and C. glabrata. Lastly, the AgNPs revealed a strong anticancer activity against SK-HEP1 liver cancer cell line with an IC50 value of 19.12 μg/mL. Conclusions: Therefore, it could be concluded that a substantial in-vivo investigations are needed for antioxidant, antibacterial and anticancer activities, so that it will be useful in medical field in future.Bidhayak ChakrabortyRaju Suresh KumarAbdulrahman I. AlmansourD. KotreshaMuthuraj RudrappaS.S. PallaviHalaswamy HiremathKarthikeyan PerumalSreenivasa NayakaElsevierarticleGalphimia glaucaGreen synthesisAntioxidantAntimicrobialAnticancerSK-HEP1 cell lineScience (General)Q1-390ENJournal of King Saud University: Science, Vol 33, Iss 8, Pp 101660- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Galphimia glauca Green synthesis Antioxidant Antimicrobial Anticancer SK-HEP1 cell line Science (General) Q1-390
spellingShingle	Galphimia glauca Green synthesis Antioxidant Antimicrobial Anticancer SK-HEP1 cell line Science (General) Q1-390 Bidhayak Chakraborty Raju Suresh Kumar Abdulrahman I. Almansour D. Kotresha Muthuraj Rudrappa S.S. Pallavi Halaswamy Hiremath Karthikeyan Perumal Sreenivasa Nayaka Evaluation of antioxidant, antimicrobial and antiproliferative activity of silver nanoparticles derived from Galphimia glauca leaf extract
description	Objective: The bio-synthesis of silver nanoparticles (AgNPs) is regarded as environment friendly and cost effective method compared to physical and chemical synthesis methods. Silver (Ag) is an important noble metal due to its tremendous use in research and medical fields throughout the world. This research work was aimed at green synthesis and characterizations of AgNPs from G. glauca leaf extract and evaluation of their bioactive potential. Methods: In the current research work, synthesis of AgNPs from G. glauca aqueous leaf extracts was performed and was characterized by UV–Visible (UV–Vis.), Fourier transform infrared (FTIR), Atomic force microscopy (AFM), Scanning electron microscopy (SEM), Energy dispersive spectroscopy (EDS), High-resolution transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), Thermo gravimetric analysis (TGA) and Zeta potential exploration. Later, antioxidant, antimicrobial activity was examined against various pathogenic organisms and anticancer activities of AgNPs were carried out against SK-HEP1 cell line. Results: The first indication of synthesis of nanoparticles was the change in color from yellowish to brown. UV–Vis. band exhibited surface plasmon resonance (SPR) at 402 nm. FTIR analysis revealed the probable bio-molecules including alkanes, alkenes, aromatics, aromatic phosphates, imine or oxime, etc. subjected to reduction of silver ions to metallic silver. The AFM, SEM and TEM analysis reported the particles were spherical shaped, poly-dispersed, 17 to 40 nm in size. The zeta potential analysis expressed a peak at –32.0 ± 0.5 mV and suggested the particles with significant long term stability. The TGA analysis revealed the stability of AgNPs at high temperature (179 ℃). The antioxidant assay of AgNPs unveiled an effective dose dependent increase in scavenging activity. Antimicrobial activity showed efficient inhibitory activity against P. aeruginosa and C. glabrata. Lastly, the AgNPs revealed a strong anticancer activity against SK-HEP1 liver cancer cell line with an IC50 value of 19.12 μg/mL. Conclusions: Therefore, it could be concluded that a substantial in-vivo investigations are needed for antioxidant, antibacterial and anticancer activities, so that it will be useful in medical field in future.
format	article
author	Bidhayak Chakraborty Raju Suresh Kumar Abdulrahman I. Almansour D. Kotresha Muthuraj Rudrappa S.S. Pallavi Halaswamy Hiremath Karthikeyan Perumal Sreenivasa Nayaka
author_facet	Bidhayak Chakraborty Raju Suresh Kumar Abdulrahman I. Almansour D. Kotresha Muthuraj Rudrappa S.S. Pallavi Halaswamy Hiremath Karthikeyan Perumal Sreenivasa Nayaka
author_sort	Bidhayak Chakraborty
title	Evaluation of antioxidant, antimicrobial and antiproliferative activity of silver nanoparticles derived from Galphimia glauca leaf extract
title_short	Evaluation of antioxidant, antimicrobial and antiproliferative activity of silver nanoparticles derived from Galphimia glauca leaf extract
title_full	Evaluation of antioxidant, antimicrobial and antiproliferative activity of silver nanoparticles derived from Galphimia glauca leaf extract
title_fullStr	Evaluation of antioxidant, antimicrobial and antiproliferative activity of silver nanoparticles derived from Galphimia glauca leaf extract
title_full_unstemmed	Evaluation of antioxidant, antimicrobial and antiproliferative activity of silver nanoparticles derived from Galphimia glauca leaf extract
title_sort	evaluation of antioxidant, antimicrobial and antiproliferative activity of silver nanoparticles derived from galphimia glauca leaf extract
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/367a482a15cc427eb9b6074a0bc9e1b5
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Evaluation of antioxidant, antimicrobial and antiproliferative activity of silver nanoparticles derived from Galphimia glauca leaf extract

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