The duo Clostridium and Lactobacillus linked to hydrogen production from a lignocellulosic substrate
The study aimed to identify interspecies interactions within a native microbial community present in a hydrogen-producing bioreactor fed with two wheat straw cultivars. The relationships between the microbial community members were studied building a canonical correspondence analysis and corroborate...
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IWA Publishing
2021
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oai:doaj.org-article:367a6fbe383a4d5fb2066cdbf72d6fc82021-11-06T11:09:56ZThe duo Clostridium and Lactobacillus linked to hydrogen production from a lignocellulosic substrate0273-12231996-973210.2166/wst.2021.186https://doaj.org/article/367a6fbe383a4d5fb2066cdbf72d6fc82021-06-01T00:00:00Zhttp://wst.iwaponline.com/content/83/12/3033https://doaj.org/toc/0273-1223https://doaj.org/toc/1996-9732The study aimed to identify interspecies interactions within a native microbial community present in a hydrogen-producing bioreactor fed with two wheat straw cultivars. The relationships between the microbial community members were studied building a canonical correspondence analysis and corroborated through in vitro assays. The results showed that the bioreactor reached a stable hydrogen production of ca. 86 mL/kg·d in which the cultivar change did not affect the average performance. Lactobacillus and Clostridium dominated throughout the whole operation period where butyric acid was the main metabolite. A canonical correspondence analysis correlated positively Lactobacillus with hydrogen productivity and hydrogen-producing bacteria like Clostridium and Ruminococaceae. Agar diffusion testing of isolated strains confirmed that Lactobacillus inhibited the growth of Enterococcus, but not of Clostridium. We suggest that the positive interaction between Lactobacillus and Clostridium is generated by a division of labor for degrading the lignocellulosic substrate in which Lactobacillus produces lactic acid from the sugar fermentation while Clostridium quickly uses this lactic acid to produce hydrogen and butyric acid. The significance of this work lies in the fact that different methodological approaches confirm a positive association in the duo Lactobacillus–Clostridium in a bioreactor with stable hydrogen production from a complex substrate. HIGHLIGHTS Native microbiota of wheat straw reached a stable hydrogen production.; Microbial structure was stable dominated by Lactobacillus and Clostridium.; Statistical analysis and in vitro assays demonstrated positive interactions.; Division of labor in lignocellulose consumption could promote positive interactions.;Marisol Pérez-RangelJosé Eleazar Barboza-CoronaMarcelo Navarro-DíazAna Elena EscalanteIdania Valdez-VazquezIWA Publishingarticleagar diffusion methodantibacterial activitybioenergycomplex substratemicrobial interactionsEnvironmental technology. Sanitary engineeringTD1-1066ENWater Science and Technology, Vol 83, Iss 12, Pp 3033-3040 (2021) |
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DOAJ |
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EN |
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agar diffusion method antibacterial activity bioenergy complex substrate microbial interactions Environmental technology. Sanitary engineering TD1-1066 |
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agar diffusion method antibacterial activity bioenergy complex substrate microbial interactions Environmental technology. Sanitary engineering TD1-1066 Marisol Pérez-Rangel José Eleazar Barboza-Corona Marcelo Navarro-Díaz Ana Elena Escalante Idania Valdez-Vazquez The duo Clostridium and Lactobacillus linked to hydrogen production from a lignocellulosic substrate |
| description |
The study aimed to identify interspecies interactions within a native microbial community present in a hydrogen-producing bioreactor fed with two wheat straw cultivars. The relationships between the microbial community members were studied building a canonical correspondence analysis and corroborated through in vitro assays. The results showed that the bioreactor reached a stable hydrogen production of ca. 86 mL/kg·d in which the cultivar change did not affect the average performance. Lactobacillus and Clostridium dominated throughout the whole operation period where butyric acid was the main metabolite. A canonical correspondence analysis correlated positively Lactobacillus with hydrogen productivity and hydrogen-producing bacteria like Clostridium and Ruminococaceae. Agar diffusion testing of isolated strains confirmed that Lactobacillus inhibited the growth of Enterococcus, but not of Clostridium. We suggest that the positive interaction between Lactobacillus and Clostridium is generated by a division of labor for degrading the lignocellulosic substrate in which Lactobacillus produces lactic acid from the sugar fermentation while Clostridium quickly uses this lactic acid to produce hydrogen and butyric acid. The significance of this work lies in the fact that different methodological approaches confirm a positive association in the duo Lactobacillus–Clostridium in a bioreactor with stable hydrogen production from a complex substrate. HIGHLIGHTS
Native microbiota of wheat straw reached a stable hydrogen production.;
Microbial structure was stable dominated by Lactobacillus and Clostridium.;
Statistical analysis and in vitro assays demonstrated positive interactions.;
Division of labor in lignocellulose consumption could promote positive interactions.; |
| format |
article |
| author |
Marisol Pérez-Rangel José Eleazar Barboza-Corona Marcelo Navarro-Díaz Ana Elena Escalante Idania Valdez-Vazquez |
| author_facet |
Marisol Pérez-Rangel José Eleazar Barboza-Corona Marcelo Navarro-Díaz Ana Elena Escalante Idania Valdez-Vazquez |
| author_sort |
Marisol Pérez-Rangel |
| title |
The duo Clostridium and Lactobacillus linked to hydrogen production from a lignocellulosic substrate |
| title_short |
The duo Clostridium and Lactobacillus linked to hydrogen production from a lignocellulosic substrate |
| title_full |
The duo Clostridium and Lactobacillus linked to hydrogen production from a lignocellulosic substrate |
| title_fullStr |
The duo Clostridium and Lactobacillus linked to hydrogen production from a lignocellulosic substrate |
| title_full_unstemmed |
The duo Clostridium and Lactobacillus linked to hydrogen production from a lignocellulosic substrate |
| title_sort |
duo clostridium and lactobacillus linked to hydrogen production from a lignocellulosic substrate |
| publisher |
IWA Publishing |
| publishDate |
2021 |
| url |
https://doaj.org/article/367a6fbe383a4d5fb2066cdbf72d6fc8 |
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