mTORC1 Signaling is a Critical Regulator of Postnatal Tendon Development

Abstract Tendons transmit contractile forces between musculoskeletal tissues. Whereas the biomechanical properties of tendons have been studied extensively, the molecular mechanisms regulating postnatal tendon development are not well understood. Here we examine the role of mTORC1 signaling in postn...

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Autores principales:	Joohyun Lim, Elda Munivez, Ming-Ming Jiang, I-Wen Song, Francis Gannon, Douglas R. Keene, Ronen Schweitzer, Brendan H. Lee, Kyu Sang Joeng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/367a8c23aabe417e9f1a82455d5fb136
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spelling	oai:doaj.org-article:367a8c23aabe417e9f1a82455d5fb1362021-12-02T15:05:07ZmTORC1 Signaling is a Critical Regulator of Postnatal Tendon Development10.1038/s41598-017-17384-02045-2322https://doaj.org/article/367a8c23aabe417e9f1a82455d5fb1362017-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-17384-0https://doaj.org/toc/2045-2322Abstract Tendons transmit contractile forces between musculoskeletal tissues. Whereas the biomechanical properties of tendons have been studied extensively, the molecular mechanisms regulating postnatal tendon development are not well understood. Here we examine the role of mTORC1 signaling in postnatal tendon development using mouse genetic approaches. Loss of mTORC1 signaling by removal of Raptor in tendons caused severe tendon defects postnatally, including decreased tendon thickness, indicating that mTORC1 is necessary for postnatal tendon development. By contrast, activation of mTORC1 signaling in tendons increased tendon cell numbers and proliferation. In addition, Tsc1 conditional knockout mice presented severely disorganized collagen fibers and neovascularization in the tendon midsubstance. Interestingly, collagen fibril diameter was significantly reduced in both Raptor and Tsc1 conditional knockout mice, albeit with variations in severity. We performed RNA-seq analysis using Achilles tendons to investigate the molecular changes underlying these tendon phenotypes. Raptor conditional knockout mice showed decreased extracellular matrix (ECM) structure-related gene expression, whereas Tsc1 conditional knockout mice exhibited changes in genes regulating TGF-β/BMP/FGF signaling, as well as in genes controlling ECM structure and disassembly. Collectively, our studies suggest that maintaining physiological levels of mTORC1 signaling is essential for postnatal tendon development and maturation.Joohyun LimElda MunivezMing-Ming JiangI-Wen SongFrancis GannonDouglas R. KeeneRonen SchweitzerBrendan H. LeeKyu Sang JoengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joohyun Lim Elda Munivez Ming-Ming Jiang I-Wen Song Francis Gannon Douglas R. Keene Ronen Schweitzer Brendan H. Lee Kyu Sang Joeng mTORC1 Signaling is a Critical Regulator of Postnatal Tendon Development
description	Abstract Tendons transmit contractile forces between musculoskeletal tissues. Whereas the biomechanical properties of tendons have been studied extensively, the molecular mechanisms regulating postnatal tendon development are not well understood. Here we examine the role of mTORC1 signaling in postnatal tendon development using mouse genetic approaches. Loss of mTORC1 signaling by removal of Raptor in tendons caused severe tendon defects postnatally, including decreased tendon thickness, indicating that mTORC1 is necessary for postnatal tendon development. By contrast, activation of mTORC1 signaling in tendons increased tendon cell numbers and proliferation. In addition, Tsc1 conditional knockout mice presented severely disorganized collagen fibers and neovascularization in the tendon midsubstance. Interestingly, collagen fibril diameter was significantly reduced in both Raptor and Tsc1 conditional knockout mice, albeit with variations in severity. We performed RNA-seq analysis using Achilles tendons to investigate the molecular changes underlying these tendon phenotypes. Raptor conditional knockout mice showed decreased extracellular matrix (ECM) structure-related gene expression, whereas Tsc1 conditional knockout mice exhibited changes in genes regulating TGF-β/BMP/FGF signaling, as well as in genes controlling ECM structure and disassembly. Collectively, our studies suggest that maintaining physiological levels of mTORC1 signaling is essential for postnatal tendon development and maturation.
format	article
author	Joohyun Lim Elda Munivez Ming-Ming Jiang I-Wen Song Francis Gannon Douglas R. Keene Ronen Schweitzer Brendan H. Lee Kyu Sang Joeng
author_facet	Joohyun Lim Elda Munivez Ming-Ming Jiang I-Wen Song Francis Gannon Douglas R. Keene Ronen Schweitzer Brendan H. Lee Kyu Sang Joeng
author_sort	Joohyun Lim
title	mTORC1 Signaling is a Critical Regulator of Postnatal Tendon Development
title_short	mTORC1 Signaling is a Critical Regulator of Postnatal Tendon Development
title_full	mTORC1 Signaling is a Critical Regulator of Postnatal Tendon Development
title_fullStr	mTORC1 Signaling is a Critical Regulator of Postnatal Tendon Development
title_full_unstemmed	mTORC1 Signaling is a Critical Regulator of Postnatal Tendon Development
title_sort	mtorc1 signaling is a critical regulator of postnatal tendon development
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/367a8c23aabe417e9f1a82455d5fb136
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mTORC1 Signaling is a Critical Regulator of Postnatal Tendon Development

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