Staphylococcus pseudintermedius’s PBP4 Is Directly Associated with the Dissociated Oxacillin and Cefoxitin Phenotype

Staphylococcus pseudintermedius is an important pathogen responsible for infections in dogs and in humans. The emergence and dissemination of methicillin-resistant S. pseudintermedius (MRSP) and the multidrug resistance frequently seen in this species make diffi...

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Autores principales:	Paula Gagetti, Roberto R. Rosato, Adriana E. Rosato
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>S. pseudintermedius</i> MRSP Penicillin-Binding Proteins (PBPs) antimicrobial resistance Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/367beff08a274f46818e3b051385540a
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spelling	oai:doaj.org-article:367beff08a274f46818e3b051385540a2021-11-25T16:22:19Z<i>Staphylococcus pseudintermedius</i>’s PBP4 Is Directly Associated with the Dissociated Oxacillin and Cefoxitin Phenotype10.3390/antibiotics101112992079-6382https://doaj.org/article/367beff08a274f46818e3b051385540a2021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1299https://doaj.org/toc/2079-6382<i>Staphylococcus pseudintermedius</i> is an important pathogen responsible for infections in dogs and in humans. The emergence and dissemination of methicillin-resistant <i>S. pseudintermedius</i> (MRSP) and the multidrug resistance frequently seen in this species make difficult the treatment of these pathogens. The cefoxitin disk is widely used as a marker of methicillin resistance mediated by the <i>mecA</i> gene in <i>Staphylococcus aureus</i> and other staphylococcal species; however, it is not useful to detect β-lactam resistance of MRSP in clinical microbiology laboratories. The purpose of this study was to elucidate the molecular bases of the dissociated phenotype between oxacillin and cefoxitin antibiotics. By using a combinatorial approach that included the Penicillin-Binding Proteins’ (PBP) profile, their affinity for different β-lactam antibiotics and the analyses of PBPs’ sequence, we provide evidence that PBP4 showed still affinity for its target cefoxitin, impairing its phenotypic resistant detection in MRSP. Together, these findings provide evidence that <i>S. pseudintermedius</i> PBP4 is directly associated with the dissociated oxacillin and cefoxitin phenotype.Paula GagettiRoberto R. RosatoAdriana E. RosatoMDPI AGarticle<i>S. pseudintermedius</i>MRSPPenicillin-Binding Proteins (PBPs)antimicrobial resistanceTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1299, p 1299 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>S. pseudintermedius</i> MRSP Penicillin-Binding Proteins (PBPs) antimicrobial resistance Therapeutics. Pharmacology RM1-950
spellingShingle	<i>S. pseudintermedius</i> MRSP Penicillin-Binding Proteins (PBPs) antimicrobial resistance Therapeutics. Pharmacology RM1-950 Paula Gagetti Roberto R. Rosato Adriana E. Rosato <i>Staphylococcus pseudintermedius</i>’s PBP4 Is Directly Associated with the Dissociated Oxacillin and Cefoxitin Phenotype
description	<i>Staphylococcus pseudintermedius</i> is an important pathogen responsible for infections in dogs and in humans. The emergence and dissemination of methicillin-resistant <i>S. pseudintermedius</i> (MRSP) and the multidrug resistance frequently seen in this species make difficult the treatment of these pathogens. The cefoxitin disk is widely used as a marker of methicillin resistance mediated by the <i>mecA</i> gene in <i>Staphylococcus aureus</i> and other staphylococcal species; however, it is not useful to detect β-lactam resistance of MRSP in clinical microbiology laboratories. The purpose of this study was to elucidate the molecular bases of the dissociated phenotype between oxacillin and cefoxitin antibiotics. By using a combinatorial approach that included the Penicillin-Binding Proteins’ (PBP) profile, their affinity for different β-lactam antibiotics and the analyses of PBPs’ sequence, we provide evidence that PBP4 showed still affinity for its target cefoxitin, impairing its phenotypic resistant detection in MRSP. Together, these findings provide evidence that <i>S. pseudintermedius</i> PBP4 is directly associated with the dissociated oxacillin and cefoxitin phenotype.
format	article
author	Paula Gagetti Roberto R. Rosato Adriana E. Rosato
author_facet	Paula Gagetti Roberto R. Rosato Adriana E. Rosato
author_sort	Paula Gagetti
title	<i>Staphylococcus pseudintermedius</i>’s PBP4 Is Directly Associated with the Dissociated Oxacillin and Cefoxitin Phenotype
title_short	<i>Staphylococcus pseudintermedius</i>’s PBP4 Is Directly Associated with the Dissociated Oxacillin and Cefoxitin Phenotype
title_full	<i>Staphylococcus pseudintermedius</i>’s PBP4 Is Directly Associated with the Dissociated Oxacillin and Cefoxitin Phenotype
title_fullStr	<i>Staphylococcus pseudintermedius</i>’s PBP4 Is Directly Associated with the Dissociated Oxacillin and Cefoxitin Phenotype
title_full_unstemmed	<i>Staphylococcus pseudintermedius</i>’s PBP4 Is Directly Associated with the Dissociated Oxacillin and Cefoxitin Phenotype
title_sort	<i>staphylococcus pseudintermedius</i>’s pbp4 is directly associated with the dissociated oxacillin and cefoxitin phenotype
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/367beff08a274f46818e3b051385540a
work_keys_str_mv	AT paulagagetti istaphylococcuspseudintermediusispbp4isdirectlyassociatedwiththedissociatedoxacillinandcefoxitinphenotype AT robertorrosato istaphylococcuspseudintermediusispbp4isdirectlyassociatedwiththedissociatedoxacillinandcefoxitinphenotype AT adrianaerosato istaphylococcuspseudintermediusispbp4isdirectlyassociatedwiththedissociatedoxacillinandcefoxitinphenotype
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<i>Staphylococcus pseudintermedius</i>’s PBP4 Is Directly Associated with the Dissociated Oxacillin and Cefoxitin Phenotype

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