Evaluation of insecticidal potential of organochemicals on SF9 cell line

Abstract Background Organophosphates and Pyrethroids are the most widely used pesticides worldwide and are known to have significant toxicity on the nervous system of the target pest. Assessment for combined toxicity of Organophosphate and Pyrethroid on Sf9 (Spodoptera frugiperda) cells is less expl...

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Autores principales:	Nishi Pandya, Bhumi Thakkar, Parth Pandya, Pragna Parikh
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Sf9 cell line Organophosphate Profex Ammo Apoptosis ROS Zoology QL1-991
Acceso en línea:	https://doaj.org/article/367c083e70c940819bd178398096df59
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spelling	oai:doaj.org-article:367c083e70c940819bd178398096df592021-11-14T12:24:46ZEvaluation of insecticidal potential of organochemicals on SF9 cell line10.1186/s41936-021-00257-42090-990Xhttps://doaj.org/article/367c083e70c940819bd178398096df592021-11-01T00:00:00Zhttps://doi.org/10.1186/s41936-021-00257-4https://doaj.org/toc/2090-990XAbstract Background Organophosphates and Pyrethroids are the most widely used pesticides worldwide and are known to have significant toxicity on the nervous system of the target pest. Assessment for combined toxicity of Organophosphate and Pyrethroid on Sf9 (Spodoptera frugiperda) cells is less explored. The present study demonstrates and compares the two organochemicals whose trade names are Ammo and Profex, for its cytotoxic potential on the insect Sf9 cells. Ammo and Profex were selected as the test chemicals as toxicity of these insecticides at molecular and cellular level is poorly understood. Results The results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay demonstrated that Ammo and Profex exhibited significant cytotoxicity to Sf9 cells in a time- and dose-dependent manner. In our study, the IC50 value was obtained by MTT assay and the sub-lethal concentrations (IC50/20-17.5 µg/ml, IC50/10-35 µg/ml, and IC50/5–70 µg/ml for Ammo and IC50/20-20 µg/ml, IC50/10-40 µg/ml, and IC50/5-80 µg/ml for Profex) were selected for further tests. Acridine orange/ethidium bromide staining proved the apoptotic cell death on exposure of both the insecticides confirming its toxic potential. Furthermore, antioxidant status was assessed using DCF-DA staining and both the insecticides resulted into an increased reactive oxygen species (ROS) generation. A dose- and time-dependent significant (p < 0.05) alterations in lipid peroxidase (LPO), glutathione (GSH) and catalase (CAT) activity were observed. Conclusion The results showed that both Ammo and Profex triggered apoptosis in Sf9 cells through an intrinsic mitochondrial pathway via the generation of ROS. Of the two insecticides, Ammo was found to be more toxic compared to Profex. The present study is important to evaluate the environmental safety and risk factors of Organochemicals’ exposure to crops and livestock.Nishi PandyaBhumi ThakkarParth PandyaPragna ParikhSpringerOpenarticleSf9 cell lineOrganophosphateProfexAmmoApoptosisROSZoologyQL1-991ENJournal of Basic and Applied Zoology, Vol 82, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Sf9 cell line Organophosphate Profex Ammo Apoptosis ROS Zoology QL1-991
spellingShingle	Sf9 cell line Organophosphate Profex Ammo Apoptosis ROS Zoology QL1-991 Nishi Pandya Bhumi Thakkar Parth Pandya Pragna Parikh Evaluation of insecticidal potential of organochemicals on SF9 cell line
description	Abstract Background Organophosphates and Pyrethroids are the most widely used pesticides worldwide and are known to have significant toxicity on the nervous system of the target pest. Assessment for combined toxicity of Organophosphate and Pyrethroid on Sf9 (Spodoptera frugiperda) cells is less explored. The present study demonstrates and compares the two organochemicals whose trade names are Ammo and Profex, for its cytotoxic potential on the insect Sf9 cells. Ammo and Profex were selected as the test chemicals as toxicity of these insecticides at molecular and cellular level is poorly understood. Results The results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay demonstrated that Ammo and Profex exhibited significant cytotoxicity to Sf9 cells in a time- and dose-dependent manner. In our study, the IC50 value was obtained by MTT assay and the sub-lethal concentrations (IC50/20-17.5 µg/ml, IC50/10-35 µg/ml, and IC50/5–70 µg/ml for Ammo and IC50/20-20 µg/ml, IC50/10-40 µg/ml, and IC50/5-80 µg/ml for Profex) were selected for further tests. Acridine orange/ethidium bromide staining proved the apoptotic cell death on exposure of both the insecticides confirming its toxic potential. Furthermore, antioxidant status was assessed using DCF-DA staining and both the insecticides resulted into an increased reactive oxygen species (ROS) generation. A dose- and time-dependent significant (p < 0.05) alterations in lipid peroxidase (LPO), glutathione (GSH) and catalase (CAT) activity were observed. Conclusion The results showed that both Ammo and Profex triggered apoptosis in Sf9 cells through an intrinsic mitochondrial pathway via the generation of ROS. Of the two insecticides, Ammo was found to be more toxic compared to Profex. The present study is important to evaluate the environmental safety and risk factors of Organochemicals’ exposure to crops and livestock.
format	article
author	Nishi Pandya Bhumi Thakkar Parth Pandya Pragna Parikh
author_facet	Nishi Pandya Bhumi Thakkar Parth Pandya Pragna Parikh
author_sort	Nishi Pandya
title	Evaluation of insecticidal potential of organochemicals on SF9 cell line
title_short	Evaluation of insecticidal potential of organochemicals on SF9 cell line
title_full	Evaluation of insecticidal potential of organochemicals on SF9 cell line
title_fullStr	Evaluation of insecticidal potential of organochemicals on SF9 cell line
title_full_unstemmed	Evaluation of insecticidal potential of organochemicals on SF9 cell line
title_sort	evaluation of insecticidal potential of organochemicals on sf9 cell line
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/367c083e70c940819bd178398096df59
work_keys_str_mv	AT nishipandya evaluationofinsecticidalpotentialoforganochemicalsonsf9cellline AT bhumithakkar evaluationofinsecticidalpotentialoforganochemicalsonsf9cellline AT parthpandya evaluationofinsecticidalpotentialoforganochemicalsonsf9cellline AT pragnaparikh evaluationofinsecticidalpotentialoforganochemicalsonsf9cellline
_version_	1718429220976197632

Evaluation of insecticidal potential of organochemicals on SF9 cell line

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