DNA damage induction during localized chronic exposure to an insoluble radioactive microparticle

Abstract Insoluble radioactive microparticles emitted by the incident at the Fukushima nuclear power plant have drawn keen interests from the viewpoint of radiation protection. Cs-bearing particles have been assumed to adhere in the long term to trachea after aspirated into respiratory system, leadi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yusuke Matsuya, Yukihiko Satou, Nobuyuki Hamada, Hiroyuki Date, Masayori Ishikawa, Tatsuhiko Sato
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3683a18c36a948bd96c6dda02fb3fc9a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3683a18c36a948bd96c6dda02fb3fc9a
record_format	dspace
spelling	oai:doaj.org-article:3683a18c36a948bd96c6dda02fb3fc9a2021-12-02T15:09:20ZDNA damage induction during localized chronic exposure to an insoluble radioactive microparticle10.1038/s41598-019-46874-62045-2322https://doaj.org/article/3683a18c36a948bd96c6dda02fb3fc9a2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-46874-6https://doaj.org/toc/2045-2322Abstract Insoluble radioactive microparticles emitted by the incident at the Fukushima nuclear power plant have drawn keen interests from the viewpoint of radiation protection. Cs-bearing particles have been assumed to adhere in the long term to trachea after aspirated into respiratory system, leading to heterogeneous dose distribution within healthy tissue around the particles. However, the biological effects posed by an insoluble radioactive particle remain unclear. Here, we show cumulative DNA damage in normal human lung cells proximal and distal to the particle (β-ray and γ-ray-dominant areas, respectively) under localized chronic exposure in comparison with uniform exposure. We put a Cs-bearing particle into a microcapillary tip and placed it onto a glass-base dish containing fibroblast or epithelial cells cultured in vitro. A Monte Carlo simulation with PHITS code provides the radial distribution of absorbed dose-rate around the particle, and subsequently we observed a significant change in nuclear γ-H2AX foci after 24 h or 48 h exposure to the particle. The nuclear foci in the cells distal to the particle increased even under low-dose-rate exposure compared with uniform exposure to 137Cs γ-rays, which was suppressed by a treatment with a scavenger of reactive oxygen species. In contrast, such focus formation was less manifested in the exposed cells proximal to the particle compared with uniform exposure. These data suggest that the localized exposure to a Cs-bearing particle leads to not only disadvantage to distal cells but also advantage to proximal cells. This study is the first to provide quantitative evaluation for the spatial distribution of DNA double strand breaks after the heterogeneous chronic exposure to a Cs-bearing particle in comparison with uniform Cs exposure.Yusuke MatsuyaYukihiko SatouNobuyuki HamadaHiroyuki DateMasayori IshikawaTatsuhiko SatoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yusuke Matsuya Yukihiko Satou Nobuyuki Hamada Hiroyuki Date Masayori Ishikawa Tatsuhiko Sato DNA damage induction during localized chronic exposure to an insoluble radioactive microparticle
description	Abstract Insoluble radioactive microparticles emitted by the incident at the Fukushima nuclear power plant have drawn keen interests from the viewpoint of radiation protection. Cs-bearing particles have been assumed to adhere in the long term to trachea after aspirated into respiratory system, leading to heterogeneous dose distribution within healthy tissue around the particles. However, the biological effects posed by an insoluble radioactive particle remain unclear. Here, we show cumulative DNA damage in normal human lung cells proximal and distal to the particle (β-ray and γ-ray-dominant areas, respectively) under localized chronic exposure in comparison with uniform exposure. We put a Cs-bearing particle into a microcapillary tip and placed it onto a glass-base dish containing fibroblast or epithelial cells cultured in vitro. A Monte Carlo simulation with PHITS code provides the radial distribution of absorbed dose-rate around the particle, and subsequently we observed a significant change in nuclear γ-H2AX foci after 24 h or 48 h exposure to the particle. The nuclear foci in the cells distal to the particle increased even under low-dose-rate exposure compared with uniform exposure to 137Cs γ-rays, which was suppressed by a treatment with a scavenger of reactive oxygen species. In contrast, such focus formation was less manifested in the exposed cells proximal to the particle compared with uniform exposure. These data suggest that the localized exposure to a Cs-bearing particle leads to not only disadvantage to distal cells but also advantage to proximal cells. This study is the first to provide quantitative evaluation for the spatial distribution of DNA double strand breaks after the heterogeneous chronic exposure to a Cs-bearing particle in comparison with uniform Cs exposure.
format	article
author	Yusuke Matsuya Yukihiko Satou Nobuyuki Hamada Hiroyuki Date Masayori Ishikawa Tatsuhiko Sato
author_facet	Yusuke Matsuya Yukihiko Satou Nobuyuki Hamada Hiroyuki Date Masayori Ishikawa Tatsuhiko Sato
author_sort	Yusuke Matsuya
title	DNA damage induction during localized chronic exposure to an insoluble radioactive microparticle
title_short	DNA damage induction during localized chronic exposure to an insoluble radioactive microparticle
title_full	DNA damage induction during localized chronic exposure to an insoluble radioactive microparticle
title_fullStr	DNA damage induction during localized chronic exposure to an insoluble radioactive microparticle
title_full_unstemmed	DNA damage induction during localized chronic exposure to an insoluble radioactive microparticle
title_sort	dna damage induction during localized chronic exposure to an insoluble radioactive microparticle
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/3683a18c36a948bd96c6dda02fb3fc9a
work_keys_str_mv	AT yusukematsuya dnadamageinductionduringlocalizedchronicexposuretoaninsolubleradioactivemicroparticle AT yukihikosatou dnadamageinductionduringlocalizedchronicexposuretoaninsolubleradioactivemicroparticle AT nobuyukihamada dnadamageinductionduringlocalizedchronicexposuretoaninsolubleradioactivemicroparticle AT hiroyukidate dnadamageinductionduringlocalizedchronicexposuretoaninsolubleradioactivemicroparticle AT masayoriishikawa dnadamageinductionduringlocalizedchronicexposuretoaninsolubleradioactivemicroparticle AT tatsuhikosato dnadamageinductionduringlocalizedchronicexposuretoaninsolubleradioactivemicroparticle
_version_	1718387805909942272

DNA damage induction during localized chronic exposure to an insoluble radioactive microparticle

Ejemplares similares