A near‐complete genome sequence of mungbean (Vigna radiata L.) provides key insights into the modern breeding program

Abstract Mungbean (Vigna radiata L.), a fast‐growing legume species, is an important source of carbohydrates and proteins in developing countries of Asia. Here, we constructed a near‐complete genome sequence of mungbean with a scaffold N50 value of 5.2 Mb and only a 0.4% gap, with a total scaffold s...

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Autores principales:	Jungmin Ha, Dani Satyawan, Haneul Jeong, Eunsoo Lee, Kang‐Heum Cho, Moon Young Kim, Suk‐Ha Lee
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Plant culture SB1-1110 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/3692f2d981e545f1ab82284eef6b71b5
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spelling	oai:doaj.org-article:3692f2d981e545f1ab82284eef6b71b52021-12-05T07:50:12ZA near‐complete genome sequence of mungbean (Vigna radiata L.) provides key insights into the modern breeding program1940-337210.1002/tpg2.20121https://doaj.org/article/3692f2d981e545f1ab82284eef6b71b52021-11-01T00:00:00Zhttps://doi.org/10.1002/tpg2.20121https://doaj.org/toc/1940-3372Abstract Mungbean (Vigna radiata L.), a fast‐growing legume species, is an important source of carbohydrates and proteins in developing countries of Asia. Here, we constructed a near‐complete genome sequence of mungbean with a scaffold N50 value of 5.2 Mb and only a 0.4% gap, with a total scaffold size of 475 Mb. We identified several misassembled pseudomolecules (Chr03, Chr04, Chr05, and Chr08) in the previous draft assembly; Chr03, Chr04, and Chr08 were assembled into one chromosome, and Chr05 was broken into two chromosomes in the improved reference genome assembly, thus providing more accurate linkage information to breeders. Additionally, using an ultra‐high‐resolution linkage map constructed based on resequencing data, we identified several quantitative trait loci (QTLs) and the underlying candidate genes affecting synchronous pod maturity (SPM). Mungbean homologs of two soybean ([Glycine max (L.) Merr.] flowering genes, E3 (phytochrome A) and J (early flowering 3), were identified as candidate genes for the QTLs, and the candidate genes for plant height, node number, and SPM showed critical nucleotide substitutions between the reference cultivar and other genotypes (landraces and wild accessions). Based on the analysis of genetic diversity among 276 accessions collected from 23 countries, we identified 36 selective sweep regions and observed that the overall genetic diversity of cultivars decreased to 30% of that in wild accessions postdomestication. The near‐complete genome sequence of mungbean represents an important resource for genome‐assisted improvement in the mungbean breeding program.Jungmin HaDani SatyawanHaneul JeongEunsoo LeeKang‐Heum ChoMoon Young KimSuk‐Ha LeeWileyarticlePlant cultureSB1-1110GeneticsQH426-470ENThe Plant Genome, Vol 14, Iss 3, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Plant culture SB1-1110 Genetics QH426-470
spellingShingle	Plant culture SB1-1110 Genetics QH426-470 Jungmin Ha Dani Satyawan Haneul Jeong Eunsoo Lee Kang‐Heum Cho Moon Young Kim Suk‐Ha Lee A near‐complete genome sequence of mungbean (Vigna radiata L.) provides key insights into the modern breeding program
description	Abstract Mungbean (Vigna radiata L.), a fast‐growing legume species, is an important source of carbohydrates and proteins in developing countries of Asia. Here, we constructed a near‐complete genome sequence of mungbean with a scaffold N50 value of 5.2 Mb and only a 0.4% gap, with a total scaffold size of 475 Mb. We identified several misassembled pseudomolecules (Chr03, Chr04, Chr05, and Chr08) in the previous draft assembly; Chr03, Chr04, and Chr08 were assembled into one chromosome, and Chr05 was broken into two chromosomes in the improved reference genome assembly, thus providing more accurate linkage information to breeders. Additionally, using an ultra‐high‐resolution linkage map constructed based on resequencing data, we identified several quantitative trait loci (QTLs) and the underlying candidate genes affecting synchronous pod maturity (SPM). Mungbean homologs of two soybean ([Glycine max (L.) Merr.] flowering genes, E3 (phytochrome A) and J (early flowering 3), were identified as candidate genes for the QTLs, and the candidate genes for plant height, node number, and SPM showed critical nucleotide substitutions between the reference cultivar and other genotypes (landraces and wild accessions). Based on the analysis of genetic diversity among 276 accessions collected from 23 countries, we identified 36 selective sweep regions and observed that the overall genetic diversity of cultivars decreased to 30% of that in wild accessions postdomestication. The near‐complete genome sequence of mungbean represents an important resource for genome‐assisted improvement in the mungbean breeding program.
format	article
author	Jungmin Ha Dani Satyawan Haneul Jeong Eunsoo Lee Kang‐Heum Cho Moon Young Kim Suk‐Ha Lee
author_facet	Jungmin Ha Dani Satyawan Haneul Jeong Eunsoo Lee Kang‐Heum Cho Moon Young Kim Suk‐Ha Lee
author_sort	Jungmin Ha
title	A near‐complete genome sequence of mungbean (Vigna radiata L.) provides key insights into the modern breeding program
title_short	A near‐complete genome sequence of mungbean (Vigna radiata L.) provides key insights into the modern breeding program
title_full	A near‐complete genome sequence of mungbean (Vigna radiata L.) provides key insights into the modern breeding program
title_fullStr	A near‐complete genome sequence of mungbean (Vigna radiata L.) provides key insights into the modern breeding program
title_full_unstemmed	A near‐complete genome sequence of mungbean (Vigna radiata L.) provides key insights into the modern breeding program
title_sort	near‐complete genome sequence of mungbean (vigna radiata l.) provides key insights into the modern breeding program
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/3692f2d981e545f1ab82284eef6b71b5
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A near‐complete genome sequence of mungbean (Vigna radiata L.) provides key insights into the modern breeding program

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