Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta

Abstract The chloroplasts of cryptophytes arose through a secondary endosymbiotic event in which a red algal endosymbiont was integrated into a previously nonphotosynthetic eukaryote. The cryptophytes retain a remnant of the endosymbiont nucleus (nucleomorph) that is replicated once in the cell cycl...

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Autores principales:	Ryo Onuma, Neha Mishra, Shin-ya Miyagishima
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/369f2048556143d6bc0b6325f794114b
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spelling	oai:doaj.org-article:369f2048556143d6bc0b6325f794114b2021-12-02T11:52:41ZRegulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta10.1038/s41598-017-02668-22045-2322https://doaj.org/article/369f2048556143d6bc0b6325f794114b2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02668-2https://doaj.org/toc/2045-2322Abstract The chloroplasts of cryptophytes arose through a secondary endosymbiotic event in which a red algal endosymbiont was integrated into a previously nonphotosynthetic eukaryote. The cryptophytes retain a remnant of the endosymbiont nucleus (nucleomorph) that is replicated once in the cell cycle along with the chloroplast. To understand how the chloroplast, nucleomorph and host cell divide in a coordinated manner, we examined the expression of genes/proteins that are related to nucleomorph replication and chloroplast division as well as the timing of nuclear and nucleomorph DNA synthesis in the cryptophyte Guillardia theta. Nucleus-encoded nucleomorph HISTONE H2A mRNA specifically accumulated during the nuclear S phase. In contrast, nucleomorph-encoded genes/proteins that are related to nucleomorph replication and chloroplast division (FtsZ) are constantly expressed throughout the cell cycle. The results of this study and previous studies on chlorarachniophytes suggest that there was a common evolutionary pattern in which an endosymbiont lost its replication cycle-dependent transcription while cell-cycle-dependent transcriptional regulation of host nuclear genes came to restrict the timing of nucleomorph replication and chloroplast division.Ryo OnumaNeha MishraShin-ya MiyagishimaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryo Onuma Neha Mishra Shin-ya Miyagishima Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta
description	Abstract The chloroplasts of cryptophytes arose through a secondary endosymbiotic event in which a red algal endosymbiont was integrated into a previously nonphotosynthetic eukaryote. The cryptophytes retain a remnant of the endosymbiont nucleus (nucleomorph) that is replicated once in the cell cycle along with the chloroplast. To understand how the chloroplast, nucleomorph and host cell divide in a coordinated manner, we examined the expression of genes/proteins that are related to nucleomorph replication and chloroplast division as well as the timing of nuclear and nucleomorph DNA synthesis in the cryptophyte Guillardia theta. Nucleus-encoded nucleomorph HISTONE H2A mRNA specifically accumulated during the nuclear S phase. In contrast, nucleomorph-encoded genes/proteins that are related to nucleomorph replication and chloroplast division (FtsZ) are constantly expressed throughout the cell cycle. The results of this study and previous studies on chlorarachniophytes suggest that there was a common evolutionary pattern in which an endosymbiont lost its replication cycle-dependent transcription while cell-cycle-dependent transcriptional regulation of host nuclear genes came to restrict the timing of nucleomorph replication and chloroplast division.
format	article
author	Ryo Onuma Neha Mishra Shin-ya Miyagishima
author_facet	Ryo Onuma Neha Mishra Shin-ya Miyagishima
author_sort	Ryo Onuma
title	Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta
title_short	Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta
title_full	Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta
title_fullStr	Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta
title_full_unstemmed	Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta
title_sort	regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte guillardia theta
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/369f2048556143d6bc0b6325f794114b
work_keys_str_mv	AT ryoonuma regulationofchloroplastandnucleomorphreplicationbythecellcycleinthecryptophyteguillardiatheta AT nehamishra regulationofchloroplastandnucleomorphreplicationbythecellcycleinthecryptophyteguillardiatheta AT shinyamiyagishima regulationofchloroplastandnucleomorphreplicationbythecellcycleinthecryptophyteguillardiatheta
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Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta

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