Controlled modification of biomolecules by ultrashort laser pulses in polar liquids

Abstract Targeted chemical modification of peptides and proteins by laser pulses in a biologically relevant environment, i.e. aqueous solvent at room temperature, allows for accurate control of biological processes. However, the traditional laser methods of control of chemical reactions are applicab...

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Autores principales:	Vitaly Gruzdev, Dmitry Korkin, Brian P. Mooney, Jesper F. Havelund, Ian Max Møller, Jay J. Thelen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/36a5e9d30bb1414ca2b94641bfe76eba
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spelling	oai:doaj.org-article:36a5e9d30bb1414ca2b94641bfe76eba2021-12-02T16:08:10ZControlled modification of biomolecules by ultrashort laser pulses in polar liquids10.1038/s41598-017-05761-82045-2322https://doaj.org/article/36a5e9d30bb1414ca2b94641bfe76eba2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05761-8https://doaj.org/toc/2045-2322Abstract Targeted chemical modification of peptides and proteins by laser pulses in a biologically relevant environment, i.e. aqueous solvent at room temperature, allows for accurate control of biological processes. However, the traditional laser methods of control of chemical reactions are applicable only to a small class of photosensitive biomolecules because of strong and ultrafast perturbations from biomolecule-solvent interactions. Here, we report excitation of harmonics of vibration modes of solvent molecules by femtosecond laser pulses to produce controlled chemical modifications of non-photosensitive peptides and proteins in polar liquids under room conditions. The principal modifications included lysine formylation and methionine sulfoxidation both of which occur with nearly 100% yield under atmospheric conditions. That modification occurred only if the laser irradiance exceeded certain threshold level. The threshold, type, and extent of the modifications were completely controlled by solvent composition, laser wavelength, and peak irradiance of ultrashort laser pulses. This approach is expected to assist in establishing rigorous control over a broad class of biological processes in cells and tissues at the molecular level.Vitaly GruzdevDmitry KorkinBrian P. MooneyJesper F. HavelundIan Max MøllerJay J. ThelenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vitaly Gruzdev Dmitry Korkin Brian P. Mooney Jesper F. Havelund Ian Max Møller Jay J. Thelen Controlled modification of biomolecules by ultrashort laser pulses in polar liquids
description	Abstract Targeted chemical modification of peptides and proteins by laser pulses in a biologically relevant environment, i.e. aqueous solvent at room temperature, allows for accurate control of biological processes. However, the traditional laser methods of control of chemical reactions are applicable only to a small class of photosensitive biomolecules because of strong and ultrafast perturbations from biomolecule-solvent interactions. Here, we report excitation of harmonics of vibration modes of solvent molecules by femtosecond laser pulses to produce controlled chemical modifications of non-photosensitive peptides and proteins in polar liquids under room conditions. The principal modifications included lysine formylation and methionine sulfoxidation both of which occur with nearly 100% yield under atmospheric conditions. That modification occurred only if the laser irradiance exceeded certain threshold level. The threshold, type, and extent of the modifications were completely controlled by solvent composition, laser wavelength, and peak irradiance of ultrashort laser pulses. This approach is expected to assist in establishing rigorous control over a broad class of biological processes in cells and tissues at the molecular level.
format	article
author	Vitaly Gruzdev Dmitry Korkin Brian P. Mooney Jesper F. Havelund Ian Max Møller Jay J. Thelen
author_facet	Vitaly Gruzdev Dmitry Korkin Brian P. Mooney Jesper F. Havelund Ian Max Møller Jay J. Thelen
author_sort	Vitaly Gruzdev
title	Controlled modification of biomolecules by ultrashort laser pulses in polar liquids
title_short	Controlled modification of biomolecules by ultrashort laser pulses in polar liquids
title_full	Controlled modification of biomolecules by ultrashort laser pulses in polar liquids
title_fullStr	Controlled modification of biomolecules by ultrashort laser pulses in polar liquids
title_full_unstemmed	Controlled modification of biomolecules by ultrashort laser pulses in polar liquids
title_sort	controlled modification of biomolecules by ultrashort laser pulses in polar liquids
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/36a5e9d30bb1414ca2b94641bfe76eba
work_keys_str_mv	AT vitalygruzdev controlledmodificationofbiomoleculesbyultrashortlaserpulsesinpolarliquids AT dmitrykorkin controlledmodificationofbiomoleculesbyultrashortlaserpulsesinpolarliquids AT brianpmooney controlledmodificationofbiomoleculesbyultrashortlaserpulsesinpolarliquids AT jesperfhavelund controlledmodificationofbiomoleculesbyultrashortlaserpulsesinpolarliquids AT ianmaxmøller controlledmodificationofbiomoleculesbyultrashortlaserpulsesinpolarliquids AT jayjthelen controlledmodificationofbiomoleculesbyultrashortlaserpulsesinpolarliquids
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Controlled modification of biomolecules by ultrashort laser pulses in polar liquids

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