First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain

Loquat (<i>Eriobotrya japonica</i>) is an important crop in Spain. To date, only one viral species, apple stem pitting virus (ASPV), has been detected in Spanish loquat orchards. In this study, the presence of additional viruses infecting this crop in Spain was investigated. RT-PCR and h...

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Autores principales:	Celia Canales, Félix Morán, Antonio Olmos, Ana Belén Ruiz-García
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ASGV CTLV ACLSV AHVd loquat HTS Botany QK1-989
Acceso en línea:	https://doaj.org/article/36af0dbbe63d47e7895d229f3ddd58fa
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spelling	oai:doaj.org-article:36af0dbbe63d47e7895d229f3ddd58fa2021-11-25T18:44:54ZFirst Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain10.3390/plants101122932223-7747https://doaj.org/article/36af0dbbe63d47e7895d229f3ddd58fa2021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2293https://doaj.org/toc/2223-7747Loquat (<i>Eriobotrya japonica</i>) is an important crop in Spain. To date, only one viral species, apple stem pitting virus (ASPV), has been detected in Spanish loquat orchards. In this study, the presence of additional viruses infecting this crop in Spain was investigated. RT-PCR and high-throughput sequencing (HTS) of symptomatic loquat plants led to first-time detection and characterization of apple stem grooving virus (ASGV), also known as citrus tatter leaf virus (CTLV), and apple chlorotic leaf spot virus (ACLSV) from Spain with description of nearly complete genomic sequences. The frequency of ACLSV infection was the highest, with over 30% of the samples testing positive and were also detected as coinfections with ASGV and ASPV, although most of the samples infected were symptomless. Studies on all the full-length sequences available in the databases were performed in order to establish the phylogenetic relationships of the Spanish isolates of these two viral species. Moreover, apple hammerhead viroid (AHVd) was also detected to infect loquat, the first host different from apple reported for this viroid to date.Celia CanalesFélix MoránAntonio OlmosAna Belén Ruiz-GarcíaMDPI AGarticleASGVCTLVACLSVAHVdloquatHTSBotanyQK1-989ENPlants, Vol 10, Iss 2293, p 2293 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ASGV CTLV ACLSV AHVd loquat HTS Botany QK1-989
spellingShingle	ASGV CTLV ACLSV AHVd loquat HTS Botany QK1-989 Celia Canales Félix Morán Antonio Olmos Ana Belén Ruiz-García First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain
description	Loquat (<i>Eriobotrya japonica</i>) is an important crop in Spain. To date, only one viral species, apple stem pitting virus (ASPV), has been detected in Spanish loquat orchards. In this study, the presence of additional viruses infecting this crop in Spain was investigated. RT-PCR and high-throughput sequencing (HTS) of symptomatic loquat plants led to first-time detection and characterization of apple stem grooving virus (ASGV), also known as citrus tatter leaf virus (CTLV), and apple chlorotic leaf spot virus (ACLSV) from Spain with description of nearly complete genomic sequences. The frequency of ACLSV infection was the highest, with over 30% of the samples testing positive and were also detected as coinfections with ASGV and ASPV, although most of the samples infected were symptomless. Studies on all the full-length sequences available in the databases were performed in order to establish the phylogenetic relationships of the Spanish isolates of these two viral species. Moreover, apple hammerhead viroid (AHVd) was also detected to infect loquat, the first host different from apple reported for this viroid to date.
format	article
author	Celia Canales Félix Morán Antonio Olmos Ana Belén Ruiz-García
author_facet	Celia Canales Félix Morán Antonio Olmos Ana Belén Ruiz-García
author_sort	Celia Canales
title	First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain
title_short	First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain
title_full	First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain
title_fullStr	First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain
title_full_unstemmed	First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain
title_sort	first detection and molecular characterization of apple stem grooving virus, apple chlorotic leaf spot virus, and apple hammerhead viroid in loquat in spain
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/36af0dbbe63d47e7895d229f3ddd58fa
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First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain

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