First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain

Loquat (<i>Eriobotrya japonica</i>) is an important crop in Spain. To date, only one viral species, apple stem pitting virus (ASPV), has been detected in Spanish loquat orchards. In this study, the presence of additional viruses infecting this crop in Spain was investigated. RT-PCR and h...

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Autores principales: Celia Canales, Félix Morán, Antonio Olmos, Ana Belén Ruiz-García
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Lenguaje:EN
Publicado: MDPI AG 2021
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Acceso en línea:https://doaj.org/article/36af0dbbe63d47e7895d229f3ddd58fa
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spelling oai:doaj.org-article:36af0dbbe63d47e7895d229f3ddd58fa2021-11-25T18:44:54ZFirst Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain10.3390/plants101122932223-7747https://doaj.org/article/36af0dbbe63d47e7895d229f3ddd58fa2021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2293https://doaj.org/toc/2223-7747Loquat (<i>Eriobotrya japonica</i>) is an important crop in Spain. To date, only one viral species, apple stem pitting virus (ASPV), has been detected in Spanish loquat orchards. In this study, the presence of additional viruses infecting this crop in Spain was investigated. RT-PCR and high-throughput sequencing (HTS) of symptomatic loquat plants led to first-time detection and characterization of apple stem grooving virus (ASGV), also known as citrus tatter leaf virus (CTLV), and apple chlorotic leaf spot virus (ACLSV) from Spain with description of nearly complete genomic sequences. The frequency of ACLSV infection was the highest, with over 30% of the samples testing positive and were also detected as coinfections with ASGV and ASPV, although most of the samples infected were symptomless. Studies on all the full-length sequences available in the databases were performed in order to establish the phylogenetic relationships of the Spanish isolates of these two viral species. Moreover, apple hammerhead viroid (AHVd) was also detected to infect loquat, the first host different from apple reported for this viroid to date.Celia CanalesFélix MoránAntonio OlmosAna Belén Ruiz-GarcíaMDPI AGarticleASGVCTLVACLSVAHVdloquatHTSBotanyQK1-989ENPlants, Vol 10, Iss 2293, p 2293 (2021)
institution DOAJ
collection DOAJ
language EN
topic ASGV
CTLV
ACLSV
AHVd
loquat
HTS
Botany
QK1-989
spellingShingle ASGV
CTLV
ACLSV
AHVd
loquat
HTS
Botany
QK1-989
Celia Canales
Félix Morán
Antonio Olmos
Ana Belén Ruiz-García
First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain
description Loquat (<i>Eriobotrya japonica</i>) is an important crop in Spain. To date, only one viral species, apple stem pitting virus (ASPV), has been detected in Spanish loquat orchards. In this study, the presence of additional viruses infecting this crop in Spain was investigated. RT-PCR and high-throughput sequencing (HTS) of symptomatic loquat plants led to first-time detection and characterization of apple stem grooving virus (ASGV), also known as citrus tatter leaf virus (CTLV), and apple chlorotic leaf spot virus (ACLSV) from Spain with description of nearly complete genomic sequences. The frequency of ACLSV infection was the highest, with over 30% of the samples testing positive and were also detected as coinfections with ASGV and ASPV, although most of the samples infected were symptomless. Studies on all the full-length sequences available in the databases were performed in order to establish the phylogenetic relationships of the Spanish isolates of these two viral species. Moreover, apple hammerhead viroid (AHVd) was also detected to infect loquat, the first host different from apple reported for this viroid to date.
format article
author Celia Canales
Félix Morán
Antonio Olmos
Ana Belén Ruiz-García
author_facet Celia Canales
Félix Morán
Antonio Olmos
Ana Belén Ruiz-García
author_sort Celia Canales
title First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain
title_short First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain
title_full First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain
title_fullStr First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain
title_full_unstemmed First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain
title_sort first detection and molecular characterization of apple stem grooving virus, apple chlorotic leaf spot virus, and apple hammerhead viroid in loquat in spain
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/36af0dbbe63d47e7895d229f3ddd58fa
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