Fermented wheat powder induces the antioxidant and detoxifying system in primary rat hepatocytes.

<h4>Unlabelled</h4>Many plants exhibit antioxidant properties which may be useful in the prevention of oxidative stress reactions, such as those mediated by the formation of free radical species in different pathological situations. In recent years a number of studies have shown that who...

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Autores principales:	Margherita La Marca, Pascale Beffy, Annalisa Pugliese, Vincenzo Longo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/36b29617dc904b27971d4dc043344529
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spelling	oai:doaj.org-article:36b29617dc904b27971d4dc0433445292021-11-18T08:39:35ZFermented wheat powder induces the antioxidant and detoxifying system in primary rat hepatocytes.1932-620310.1371/journal.pone.0083538https://doaj.org/article/36b29617dc904b27971d4dc0433445292013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24391783/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Unlabelled</h4>Many plants exhibit antioxidant properties which may be useful in the prevention of oxidative stress reactions, such as those mediated by the formation of free radical species in different pathological situations. In recent years a number of studies have shown that whole grain products in particular have strong antioxidant activity. Primary cultures of rat hepatocytes were used to investigate whether and how a fermented powder of wheat (Lisosan G) is able to modulate antioxidant and detoxifying enzymes, and whether or not it can activate Nrf2 transcription factor or inhibit NF-kB activation. All of the antioxidant and detoxifying enzymes studied were significantly up-regulated by 0.7 mg/ml Lisosan G treatment. In particular,<h4>Nad(p)h</h4>quinone oxidoreductase and heme oxygenase-1 were induced, although to different degrees, at the transcriptional, protein and/or activity levels by the treatment. As for the Nrf2 transcription factor, a partial translocation of its protein from the cytosol to the nucleus after 1 h of Lisosan G treatment was revealed by immunoblotting. Lisosan G was also observed to decrease H2O2-induced toxicity Taken together, these results show that this powder of wheat is an effective inducer of ARE/Nrf2-regulated antioxidant and detoxifying genes and has the potential to inhibit the translocation of NF-kB into the nucleus.Margherita La MarcaPascale BeffyAnnalisa PuglieseVincenzo LongoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e83538 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Margherita La Marca Pascale Beffy Annalisa Pugliese Vincenzo Longo Fermented wheat powder induces the antioxidant and detoxifying system in primary rat hepatocytes.
description	<h4>Unlabelled</h4>Many plants exhibit antioxidant properties which may be useful in the prevention of oxidative stress reactions, such as those mediated by the formation of free radical species in different pathological situations. In recent years a number of studies have shown that whole grain products in particular have strong antioxidant activity. Primary cultures of rat hepatocytes were used to investigate whether and how a fermented powder of wheat (Lisosan G) is able to modulate antioxidant and detoxifying enzymes, and whether or not it can activate Nrf2 transcription factor or inhibit NF-kB activation. All of the antioxidant and detoxifying enzymes studied were significantly up-regulated by 0.7 mg/ml Lisosan G treatment. In particular,<h4>Nad(p)h</h4>quinone oxidoreductase and heme oxygenase-1 were induced, although to different degrees, at the transcriptional, protein and/or activity levels by the treatment. As for the Nrf2 transcription factor, a partial translocation of its protein from the cytosol to the nucleus after 1 h of Lisosan G treatment was revealed by immunoblotting. Lisosan G was also observed to decrease H2O2-induced toxicity Taken together, these results show that this powder of wheat is an effective inducer of ARE/Nrf2-regulated antioxidant and detoxifying genes and has the potential to inhibit the translocation of NF-kB into the nucleus.
format	article
author	Margherita La Marca Pascale Beffy Annalisa Pugliese Vincenzo Longo
author_facet	Margherita La Marca Pascale Beffy Annalisa Pugliese Vincenzo Longo
author_sort	Margherita La Marca
title	Fermented wheat powder induces the antioxidant and detoxifying system in primary rat hepatocytes.
title_short	Fermented wheat powder induces the antioxidant and detoxifying system in primary rat hepatocytes.
title_full	Fermented wheat powder induces the antioxidant and detoxifying system in primary rat hepatocytes.
title_fullStr	Fermented wheat powder induces the antioxidant and detoxifying system in primary rat hepatocytes.
title_full_unstemmed	Fermented wheat powder induces the antioxidant and detoxifying system in primary rat hepatocytes.
title_sort	fermented wheat powder induces the antioxidant and detoxifying system in primary rat hepatocytes.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/36b29617dc904b27971d4dc043344529
work_keys_str_mv	AT margheritalamarca fermentedwheatpowderinducestheantioxidantanddetoxifyingsysteminprimaryrathepatocytes AT pascalebeffy fermentedwheatpowderinducestheantioxidantanddetoxifyingsysteminprimaryrathepatocytes AT annalisapugliese fermentedwheatpowderinducestheantioxidantanddetoxifyingsysteminprimaryrathepatocytes AT vincenzolongo fermentedwheatpowderinducestheantioxidantanddetoxifyingsysteminprimaryrathepatocytes
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Fermented wheat powder induces the antioxidant and detoxifying system in primary rat hepatocytes.

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