Molecular identification of bacteria isolated from culture medium of rotifer fed on fishery waste diet

Abstract. Wullur S, Napitupulu H, Wantania LL, Ginting EL, Warouw V, Tallei TE, Rumengan IFM. 2020. Molecular identification of bacteria isolated from culture medium of rotifer fed on fishery waste diet. Biodiversitas 21: 2735-2740. The aim of this study was 16S-rRNA sequences based molecular identi...

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Autores principales:	Stenly Wullur, HATOPAN NAPITUPULU, LETHA LOISE WANTANIA, ELVY LIKE GINTING, VEIBE WAROUW, SANDRA TILAAR, TRINA EKAWATI TALLEI, INNEKE FENNY MELKE RUMENGAN
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	bacteria, diet, fish-waste, identification, rotifer, 16s rrna Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/36b803e2e85e467d94da03460125a8a8
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spelling	oai:doaj.org-article:36b803e2e85e467d94da03460125a8a82021-11-22T00:40:46ZMolecular identification of bacteria isolated from culture medium of rotifer fed on fishery waste diet1412-033X2085-472210.13057/biodiv/d210649https://doaj.org/article/36b803e2e85e467d94da03460125a8a82020-05-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5778https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Wullur S, Napitupulu H, Wantania LL, Ginting EL, Warouw V, Tallei TE, Rumengan IFM. 2020. Molecular identification of bacteria isolated from culture medium of rotifer fed on fishery waste diet. Biodiversitas 21: 2735-2740. The aim of this study was 16S-rRNA sequences based molecular identification of bacteria isolated from culture medium of rotifer fed with fishery waste diet (FWD). We cultured rotifer Brachionus rotundiformis in sterilized seawater (salinity 25 ppt) using FWD, following the procedure in Patent No. P00201609066. Bacteria from the culture were collected, homogenized, diluted 10 to 1000 fold, spread on agar plates and incubated at 370C for 24 to 48 hours. Representative colonies of the bacteria according to their morphologies were isolated for further characterization. Genomic DNA of the isolates were extracted, and the 16S rRNA gene of the isolates were amplified. Polymerase Chain Reaction (PCR) product of each isolate was sequenced and queried against the NCBI GenBank database. Six different isolates based on size, color, elevation, margin, and colony were observed during 24-48 hours incubation at 370C. The 16S rRNA genes of the six isolates were successfully amplified and produced DNA band at 1300-1500 bp, with quality value equal to or greater than 20 (QV20+) of each entire sequence around 941-1253 bases. Basic Local Alignment Search Tool (BLAST) queries in the NCBI GenBank and EzBioCloud database using the 16S-rRNA gene sequences showed that the six isolates belong to four different genera, i.e: Bacillus, Staphylococcus, Vibrio, and Alteromonas.Stenly WullurHATOPAN NAPITUPULULETHA LOISE WANTANIAELVY LIKE GINTINGVEIBE WAROUWSANDRA TILAARTRINA EKAWATI TALLEIINNEKE FENNY MELKE RUMENGANMBI & UNS Soloarticlebacteria, diet, fish-waste, identification, rotifer, 16s rrnaBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 6 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteria, diet, fish-waste, identification, rotifer, 16s rrna Biology (General) QH301-705.5
spellingShingle	bacteria, diet, fish-waste, identification, rotifer, 16s rrna Biology (General) QH301-705.5 Stenly Wullur HATOPAN NAPITUPULU LETHA LOISE WANTANIA ELVY LIKE GINTING VEIBE WAROUW SANDRA TILAAR TRINA EKAWATI TALLEI INNEKE FENNY MELKE RUMENGAN Molecular identification of bacteria isolated from culture medium of rotifer fed on fishery waste diet
description	Abstract. Wullur S, Napitupulu H, Wantania LL, Ginting EL, Warouw V, Tallei TE, Rumengan IFM. 2020. Molecular identification of bacteria isolated from culture medium of rotifer fed on fishery waste diet. Biodiversitas 21: 2735-2740. The aim of this study was 16S-rRNA sequences based molecular identification of bacteria isolated from culture medium of rotifer fed with fishery waste diet (FWD). We cultured rotifer Brachionus rotundiformis in sterilized seawater (salinity 25 ppt) using FWD, following the procedure in Patent No. P00201609066. Bacteria from the culture were collected, homogenized, diluted 10 to 1000 fold, spread on agar plates and incubated at 370C for 24 to 48 hours. Representative colonies of the bacteria according to their morphologies were isolated for further characterization. Genomic DNA of the isolates were extracted, and the 16S rRNA gene of the isolates were amplified. Polymerase Chain Reaction (PCR) product of each isolate was sequenced and queried against the NCBI GenBank database. Six different isolates based on size, color, elevation, margin, and colony were observed during 24-48 hours incubation at 370C. The 16S rRNA genes of the six isolates were successfully amplified and produced DNA band at 1300-1500 bp, with quality value equal to or greater than 20 (QV20+) of each entire sequence around 941-1253 bases. Basic Local Alignment Search Tool (BLAST) queries in the NCBI GenBank and EzBioCloud database using the 16S-rRNA gene sequences showed that the six isolates belong to four different genera, i.e: Bacillus, Staphylococcus, Vibrio, and Alteromonas.
format	article
author	Stenly Wullur HATOPAN NAPITUPULU LETHA LOISE WANTANIA ELVY LIKE GINTING VEIBE WAROUW SANDRA TILAAR TRINA EKAWATI TALLEI INNEKE FENNY MELKE RUMENGAN
author_facet	Stenly Wullur HATOPAN NAPITUPULU LETHA LOISE WANTANIA ELVY LIKE GINTING VEIBE WAROUW SANDRA TILAAR TRINA EKAWATI TALLEI INNEKE FENNY MELKE RUMENGAN
author_sort	Stenly Wullur
title	Molecular identification of bacteria isolated from culture medium of rotifer fed on fishery waste diet
title_short	Molecular identification of bacteria isolated from culture medium of rotifer fed on fishery waste diet
title_full	Molecular identification of bacteria isolated from culture medium of rotifer fed on fishery waste diet
title_fullStr	Molecular identification of bacteria isolated from culture medium of rotifer fed on fishery waste diet
title_full_unstemmed	Molecular identification of bacteria isolated from culture medium of rotifer fed on fishery waste diet
title_sort	molecular identification of bacteria isolated from culture medium of rotifer fed on fishery waste diet
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/36b803e2e85e467d94da03460125a8a8
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Molecular identification of bacteria isolated from culture medium of rotifer fed on fishery waste diet

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