Nanoparticle-Based Vaccines for Brucellosis: Calcium Phosphate Nanoparticles-Adsorbed Antigens Induce Cross Protective Response in Mice

Zohre Sadeghi,1 Mahdi Fasihi-Ramandi,2 Saeid Bouzari1 1Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran; 2Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IranCorrespondence: Saeid BouzariDepartment of Molecular Biology, Pasteur Institut...

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Autores principales:	Sadeghi Z, Fasihi-Ramandi M, Bouzari S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	calcium phosphate nanoparticle adjuvant brucella vaccine immune response Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/36c5f43d2384467ba0628bb9043d347c
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spelling	oai:doaj.org-article:36c5f43d2384467ba0628bb9043d347c2021-12-02T13:13:12ZNanoparticle-Based Vaccines for Brucellosis: Calcium Phosphate Nanoparticles-Adsorbed Antigens Induce Cross Protective Response in Mice1178-2013https://doaj.org/article/36c5f43d2384467ba0628bb9043d347c2020-05-01T00:00:00Zhttps://www.dovepress.com/nanoparticle-based-vaccines-for-brucellosis-calcium-phosphate-nanopart-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zohre Sadeghi,1 Mahdi Fasihi-Ramandi,2 Saeid Bouzari1 1Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran; 2Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IranCorrespondence: Saeid BouzariDepartment of Molecular Biology, Pasteur Institute of Iran, Tehran, IranTel +98 21 6696 9297Fax +98 21 66492619Email bouzari@pasteur.ac.irIntroduction: Vaccine formulation with appropriate adjuvants is an attractive approach to develop protective immunity against pathogens. Calcium phosphate nanoparticles (CaPNs) are considered as ideal adjuvants and delivery systems because of their great potential for enhancing immune responses. In the current study, we have designed nanoparticle-based vaccine candidates to induce immune responses and protection against B. melitensis and B. abortus.Materials and Methods: For this purpose, we used three Brucella antigens (FliC, 7α-HSDH, BhuA) and two multi-epitopes (poly B and poly T) absorbed by CaPNs. The efficacy of each formulation was evaluated by measuring humoral, cellular and protective responses in immunized mice.Results: The CaPNs showed an average size of about 90 nm with spherical shape and smooth surface. The CaPNs-adsorbed proteins displayed significant increase in cellular and humoral immune responses compared to the control groups. In addition, our results showed increased ratio of specific IgG2a (associated with Th1) to specific IgG1 (associated with Th2). Also, immunized mice with different vaccine candidate formulations were protected against B. melitensis 16M and B. abortus 544, and showed same levels of protection as commercial vaccines (B. melitensis Rev.1 and B. abortus RB51) except for BhuA-CaPNs.Discussion: Our data support the hypothesis that these antigens absorbed with CaPNs could be effective vaccine candidates against B. melitensis and B. abortus.Keywords: calcium phosphate nanoparticle, adjuvant, Brucella, vaccine, immune responseSadeghi ZFasihi-Ramandi MBouzari SDove Medical Pressarticlecalcium phosphate nanoparticleadjuvantbrucellavaccineimmune responseMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 3877-3886 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	calcium phosphate nanoparticle adjuvant brucella vaccine immune response Medicine (General) R5-920
spellingShingle	calcium phosphate nanoparticle adjuvant brucella vaccine immune response Medicine (General) R5-920 Sadeghi Z Fasihi-Ramandi M Bouzari S Nanoparticle-Based Vaccines for Brucellosis: Calcium Phosphate Nanoparticles-Adsorbed Antigens Induce Cross Protective Response in Mice
description	Zohre Sadeghi,1 Mahdi Fasihi-Ramandi,2 Saeid Bouzari1 1Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran; 2Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IranCorrespondence: Saeid BouzariDepartment of Molecular Biology, Pasteur Institute of Iran, Tehran, IranTel +98 21 6696 9297Fax +98 21 66492619Email bouzari@pasteur.ac.irIntroduction: Vaccine formulation with appropriate adjuvants is an attractive approach to develop protective immunity against pathogens. Calcium phosphate nanoparticles (CaPNs) are considered as ideal adjuvants and delivery systems because of their great potential for enhancing immune responses. In the current study, we have designed nanoparticle-based vaccine candidates to induce immune responses and protection against B. melitensis and B. abortus.Materials and Methods: For this purpose, we used three Brucella antigens (FliC, 7α-HSDH, BhuA) and two multi-epitopes (poly B and poly T) absorbed by CaPNs. The efficacy of each formulation was evaluated by measuring humoral, cellular and protective responses in immunized mice.Results: The CaPNs showed an average size of about 90 nm with spherical shape and smooth surface. The CaPNs-adsorbed proteins displayed significant increase in cellular and humoral immune responses compared to the control groups. In addition, our results showed increased ratio of specific IgG2a (associated with Th1) to specific IgG1 (associated with Th2). Also, immunized mice with different vaccine candidate formulations were protected against B. melitensis 16M and B. abortus 544, and showed same levels of protection as commercial vaccines (B. melitensis Rev.1 and B. abortus RB51) except for BhuA-CaPNs.Discussion: Our data support the hypothesis that these antigens absorbed with CaPNs could be effective vaccine candidates against B. melitensis and B. abortus.Keywords: calcium phosphate nanoparticle, adjuvant, Brucella, vaccine, immune response
format	article
author	Sadeghi Z Fasihi-Ramandi M Bouzari S
author_facet	Sadeghi Z Fasihi-Ramandi M Bouzari S
author_sort	Sadeghi Z
title	Nanoparticle-Based Vaccines for Brucellosis: Calcium Phosphate Nanoparticles-Adsorbed Antigens Induce Cross Protective Response in Mice
title_short	Nanoparticle-Based Vaccines for Brucellosis: Calcium Phosphate Nanoparticles-Adsorbed Antigens Induce Cross Protective Response in Mice
title_full	Nanoparticle-Based Vaccines for Brucellosis: Calcium Phosphate Nanoparticles-Adsorbed Antigens Induce Cross Protective Response in Mice
title_fullStr	Nanoparticle-Based Vaccines for Brucellosis: Calcium Phosphate Nanoparticles-Adsorbed Antigens Induce Cross Protective Response in Mice
title_full_unstemmed	Nanoparticle-Based Vaccines for Brucellosis: Calcium Phosphate Nanoparticles-Adsorbed Antigens Induce Cross Protective Response in Mice
title_sort	nanoparticle-based vaccines for brucellosis: calcium phosphate nanoparticles-adsorbed antigens induce cross protective response in mice
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/36c5f43d2384467ba0628bb9043d347c
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Nanoparticle-Based Vaccines for Brucellosis: Calcium Phosphate Nanoparticles-Adsorbed Antigens Induce Cross Protective Response in Mice

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