Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold

Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold

Lu Cao,1,2 Ping-Guo Duan,1,2 Hui-Ren Wang,1,2 Xi-Lei Li,1,2 Feng-Lai Yuan,3 Zhong-Yong Fan,4 Su-Ming Li,5 Jian Dong1,21Department of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China; 2State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, Chin...

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Autores principales: Cao L, Duan PG, Wang HR, Li XL, Yuan FL, Fan ZY, Li SM, Dong J
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2012
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/36c67a992c044a0e9367b6abb6ad1689
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spelling oai:doaj.org-article:36c67a992c044a0e9367b6abb6ad16892021-12-02T00:52:04ZDegradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold1176-91141178-2013https://doaj.org/article/36c67a992c044a0e9367b6abb6ad16892012-11-01T00:00:00Zhttp://www.dovepress.com/degradation-and-osteogenic-potential-of-a-novel-polylactic-acidnano-si-a11629https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Lu Cao,1,2 Ping-Guo Duan,1,2 Hui-Ren Wang,1,2 Xi-Lei Li,1,2 Feng-Lai Yuan,3 Zhong-Yong Fan,4 Su-Ming Li,5 Jian Dong1,21Department of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China; 2State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, China; 3Affiliated Third Hospital of Nantong University, Wuxi, Jiangsu, China; 4Department of Materials Science, Fudan University, Shanghai, China; 5Max Mousseron Institute on Biomolecules, Montpellier I University, Montpellier, FranceAbstract: The purpose of this study was to investigate the influence of nano-sized β-tricalcium phosphate (β-TCP) on the biological performance of poly (lactic acid) (PLA) composite scaffolds by using in vitro degradation and an in vivo model of heterotopic bone formation. Nano-sized βTCP (nβ-TCP) was prepared with a wet grinding method from micro-sized β-TCP (mβ-TCP), and composite scaffolds containing 0, 10, 30, or 50 wt% nβ-TCP or 30 wt% mβ -TCP were generated using a freeze-drying method. Degradation was assessed by monitoring changes in microstructure, pH, weight, and compressive strength over a 26-week period of hydrolysis. Composite scaffolds were processed into blocks, and implanted into muscular pockets of rabbits after loading with recombinant human bone morphogenetic protein-2 (rhBMP-2). New bone formation was evaluated based on histological and immunohistochemical analysis 2, 4, and 8 weeks after implantation. The in vitro results indicated that the buffering effect of nβ-TCP was stronger than mβ-TCP, which was positively correlated with the content of nβ-TCP. The in vivo findings demonstrated that nβ-TCP enhanced the osteoconductivity of the scaffolds. Although composite scaffolds containing 30% nβ-TCP exhibited similar osteoconductivity to 50% nβ-TCP, they had better mechanical properties than the 50% nβ-TCP scaffolds. This study supports the potential application of a composite scaffold containing 30% nβ-TCP as a promising scaffold for bone regeneration.Keywords: poly (lactic acid), β-tricalcium phosphate, biodegradation, porous scaffold, bone regenerationCao LDuan PGWang HRLi XLYuan FLFan ZYLi SMDong JDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 5881-5888 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Cao L
Duan PG
Wang HR
Li XL
Yuan FL
Fan ZY
Li SM
Dong J
Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold
description Lu Cao,1,2 Ping-Guo Duan,1,2 Hui-Ren Wang,1,2 Xi-Lei Li,1,2 Feng-Lai Yuan,3 Zhong-Yong Fan,4 Su-Ming Li,5 Jian Dong1,21Department of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China; 2State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, China; 3Affiliated Third Hospital of Nantong University, Wuxi, Jiangsu, China; 4Department of Materials Science, Fudan University, Shanghai, China; 5Max Mousseron Institute on Biomolecules, Montpellier I University, Montpellier, FranceAbstract: The purpose of this study was to investigate the influence of nano-sized β-tricalcium phosphate (β-TCP) on the biological performance of poly (lactic acid) (PLA) composite scaffolds by using in vitro degradation and an in vivo model of heterotopic bone formation. Nano-sized βTCP (nβ-TCP) was prepared with a wet grinding method from micro-sized β-TCP (mβ-TCP), and composite scaffolds containing 0, 10, 30, or 50 wt% nβ-TCP or 30 wt% mβ -TCP were generated using a freeze-drying method. Degradation was assessed by monitoring changes in microstructure, pH, weight, and compressive strength over a 26-week period of hydrolysis. Composite scaffolds were processed into blocks, and implanted into muscular pockets of rabbits after loading with recombinant human bone morphogenetic protein-2 (rhBMP-2). New bone formation was evaluated based on histological and immunohistochemical analysis 2, 4, and 8 weeks after implantation. The in vitro results indicated that the buffering effect of nβ-TCP was stronger than mβ-TCP, which was positively correlated with the content of nβ-TCP. The in vivo findings demonstrated that nβ-TCP enhanced the osteoconductivity of the scaffolds. Although composite scaffolds containing 30% nβ-TCP exhibited similar osteoconductivity to 50% nβ-TCP, they had better mechanical properties than the 50% nβ-TCP scaffolds. This study supports the potential application of a composite scaffold containing 30% nβ-TCP as a promising scaffold for bone regeneration.Keywords: poly (lactic acid), β-tricalcium phosphate, biodegradation, porous scaffold, bone regeneration
format article
author Cao L
Duan PG
Wang HR
Li XL
Yuan FL
Fan ZY
Li SM
Dong J
author_facet Cao L
Duan PG
Wang HR
Li XL
Yuan FL
Fan ZY
Li SM
Dong J
author_sort Cao L
title Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold
title_short Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold
title_full Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold
title_fullStr Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold
title_full_unstemmed Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold
title_sort degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold
publisher Dove Medical Press
publishDate 2012
url https://doaj.org/article/36c67a992c044a0e9367b6abb6ad1689
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