An integrated strategy by using target tissue metabolomics biomarkers as pharmacodynamic surrogate indices to screen antipyretic components of Qingkaikling injection

Abstract Traditional Chinese medicine (TCM) treatment can be valuable therapeutic strategies. However, the active components and action mechanisms that account for its therapeutic effects remain elusive. Based on the hypothesis that the components of a formula which exert effect would be measurable...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Zhixin Zhang, Fang Lu, Haiyu Liu, Huizhen Zhao, Yuehong Liu, Shuang Fu, Meiling Wang, Ziye Xie, Honghong Yu, Zhenghai Huang, Yanling Zhang, Xiaoyan Gao
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
Materias:
R
Q
Acceso en línea:https://doaj.org/article/36c9b9030ea84df0b4f1d2160ba86113
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:36c9b9030ea84df0b4f1d2160ba86113
record_format dspace
spelling oai:doaj.org-article:36c9b9030ea84df0b4f1d2160ba861132021-12-02T15:05:41ZAn integrated strategy by using target tissue metabolomics biomarkers as pharmacodynamic surrogate indices to screen antipyretic components of Qingkaikling injection10.1038/s41598-017-05812-02045-2322https://doaj.org/article/36c9b9030ea84df0b4f1d2160ba861132017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05812-0https://doaj.org/toc/2045-2322Abstract Traditional Chinese medicine (TCM) treatment can be valuable therapeutic strategies. However, the active components and action mechanisms that account for its therapeutic effects remain elusive. Based on the hypothesis that the components of a formula which exert effect would be measurable in target tissue, a target tissue metabolomics-based strategy was proposed for screening of antipyretic components in Qingkaikling injection (QKLI). First, we detected the components of QKLI which could reach its target tissue (hypothalamus) by determining the hypothalamus microdialysate and discovered that only baicalin and geniposide could be detected. Then, by conducting hypothalamus metabolomics studies, 14 metabolites were screened as the potential biomarkers that related to the antipyretic mechanisms of QKLI and were used as its pharmacodynamic surrogate indices. Subsequently, the dynamic concentration of baicalin and geniposide in hypothalamus microdialysates and biomarkers in hypothalamus were measured and correlated with each other. The results indicated that only baicalin shown a good correlation with these biomarkers. Finally, a network pharmacology approach was established to validate the antipyretic activity of baicalin and the results elucidated its antipyretic mechanisms as well. The integrated strategy proposed here provided a powerful means for identifying active components and mechanisms contributing to pharmacological effects of TCM.Zhixin ZhangFang LuHaiyu LiuHuizhen ZhaoYuehong LiuShuang FuMeiling WangZiye XieHonghong YuZhenghai HuangYanling ZhangXiaoyan GaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Zhixin Zhang
Fang Lu
Haiyu Liu
Huizhen Zhao
Yuehong Liu
Shuang Fu
Meiling Wang
Ziye Xie
Honghong Yu
Zhenghai Huang
Yanling Zhang
Xiaoyan Gao
An integrated strategy by using target tissue metabolomics biomarkers as pharmacodynamic surrogate indices to screen antipyretic components of Qingkaikling injection
description Abstract Traditional Chinese medicine (TCM) treatment can be valuable therapeutic strategies. However, the active components and action mechanisms that account for its therapeutic effects remain elusive. Based on the hypothesis that the components of a formula which exert effect would be measurable in target tissue, a target tissue metabolomics-based strategy was proposed for screening of antipyretic components in Qingkaikling injection (QKLI). First, we detected the components of QKLI which could reach its target tissue (hypothalamus) by determining the hypothalamus microdialysate and discovered that only baicalin and geniposide could be detected. Then, by conducting hypothalamus metabolomics studies, 14 metabolites were screened as the potential biomarkers that related to the antipyretic mechanisms of QKLI and were used as its pharmacodynamic surrogate indices. Subsequently, the dynamic concentration of baicalin and geniposide in hypothalamus microdialysates and biomarkers in hypothalamus were measured and correlated with each other. The results indicated that only baicalin shown a good correlation with these biomarkers. Finally, a network pharmacology approach was established to validate the antipyretic activity of baicalin and the results elucidated its antipyretic mechanisms as well. The integrated strategy proposed here provided a powerful means for identifying active components and mechanisms contributing to pharmacological effects of TCM.
format article
author Zhixin Zhang
Fang Lu
Haiyu Liu
Huizhen Zhao
Yuehong Liu
Shuang Fu
Meiling Wang
Ziye Xie
Honghong Yu
Zhenghai Huang
Yanling Zhang
Xiaoyan Gao
author_facet Zhixin Zhang
Fang Lu
Haiyu Liu
Huizhen Zhao
Yuehong Liu
Shuang Fu
Meiling Wang
Ziye Xie
Honghong Yu
Zhenghai Huang
Yanling Zhang
Xiaoyan Gao
author_sort Zhixin Zhang
title An integrated strategy by using target tissue metabolomics biomarkers as pharmacodynamic surrogate indices to screen antipyretic components of Qingkaikling injection
title_short An integrated strategy by using target tissue metabolomics biomarkers as pharmacodynamic surrogate indices to screen antipyretic components of Qingkaikling injection
title_full An integrated strategy by using target tissue metabolomics biomarkers as pharmacodynamic surrogate indices to screen antipyretic components of Qingkaikling injection
title_fullStr An integrated strategy by using target tissue metabolomics biomarkers as pharmacodynamic surrogate indices to screen antipyretic components of Qingkaikling injection
title_full_unstemmed An integrated strategy by using target tissue metabolomics biomarkers as pharmacodynamic surrogate indices to screen antipyretic components of Qingkaikling injection
title_sort integrated strategy by using target tissue metabolomics biomarkers as pharmacodynamic surrogate indices to screen antipyretic components of qingkaikling injection
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/36c9b9030ea84df0b4f1d2160ba86113
work_keys_str_mv AT zhixinzhang anintegratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT fanglu anintegratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT haiyuliu anintegratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT huizhenzhao anintegratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT yuehongliu anintegratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT shuangfu anintegratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT meilingwang anintegratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT ziyexie anintegratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT honghongyu anintegratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT zhenghaihuang anintegratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT yanlingzhang anintegratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT xiaoyangao anintegratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT zhixinzhang integratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT fanglu integratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT haiyuliu integratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT huizhenzhao integratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT yuehongliu integratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT shuangfu integratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT meilingwang integratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT ziyexie integratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT honghongyu integratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT zhenghaihuang integratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT yanlingzhang integratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
AT xiaoyangao integratedstrategybyusingtargettissuemetabolomicsbiomarkersaspharmacodynamicsurrogateindicestoscreenantipyreticcomponentsofqingkaiklinginjection
_version_ 1718388758394437632