Study of the effect of dermatological phytogel on the ability of microorganisms to form a biofilm

The development of many chronic infections, including skin diseases, is caused by bacteria growing in the form of biofilms. Bacterial biofilms provide beneficial survival mechanisms that determine virulence, disease pathogenesis, or resistance of the pathogen to antibiotics. As shown by a large numb...

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Autores principales:	V. S. Myrhorod, N. I. Filimonova, O. G. Bashura, S. G. Bobro
Formato:	article
Lenguaje:	UK
Publicado:	The State Expert Center of the Ministry of Health of Ukraine 2021
Materias:	biofilms dry walnut leaf extract dry nettle extract dry thyme extract gel Therapeutics. Pharmacology RM1-950 Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/36cc6c0a8b794a13a6e68b6ffa72e5ba
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spelling	oai:doaj.org-article:36cc6c0a8b794a13a6e68b6ffa72e5ba2021-11-04T12:27:59ZStudy of the effect of dermatological phytogel on the ability of microorganisms to form a biofilm10.32352/0367-3057.5.21.070367-30572617-9628https://doaj.org/article/36cc6c0a8b794a13a6e68b6ffa72e5ba2021-10-01T00:00:00Zhttps://pharmj.org.ua/index.php/journal/article/view/1251/1175https://doaj.org/toc/0367-3057https://doaj.org/toc/2617-9628The development of many chronic infections, including skin diseases, is caused by bacteria growing in the form of biofilms. Bacterial biofilms provide beneficial survival mechanisms that determine virulence, disease pathogenesis, or resistance of the pathogen to antibiotics. As shown by a large number of studies, biofilms play an important role in the pathogenesis of dermatological diseases, including atopic dermatitis. The close relationship between the microbial biofilm that colonizes the skin surface and the negative consequences for human health makes the skin microbiome an object of therapeutic intervention in dermatological pathogenic processes. The work aims to study the effect of dermatological phytogel on the ability of microorganisms to form biofilms. The objects of research were samples of gel containing dry walnut leaf extract with the sum of tannins in terms of gallic acid and dry matter 30 mg/100 g of gel, dry nettle extract with the sum of hydroxycinnamic acids in terms of chlorogenic acid, and dry matter 20 mg/100 g of gel, dry thyme extract with the sum of flavonoids in terms of rutin and dry matter 35 mg/100 g of gel both monocomponent and combined. The study of the ability of individual plant components of phytogel samples N 1, N 2, N 3 and samples of combined phytogel N 4, N 5, and N 6 to influence biofilm formation have shown that the most pronounced decceleration of biofilms formation was registered in the gel sample with phytocomplex N 4 and was 19.7–20.7% to S. aureus, E. coli, P. aerugenosis and C. albicans respectively. The activity of the gel sample with phytocomplex N 4 was 1.3–1.4 times higher than that of monocomponent gel samples N 1, N 2, and N 3. When determining the ability of the test samples to destroy biofilms, it has been found that the gel sample with phytocomplex N 4 showed the greatest activity, which exceeded the specified properties of samples N 5 and N 6 by an average of 1.2 and 1.8 times. The activity of single-component gel samples N 1, N 2, and N 3 was lower in S. aureus, E. coli, P. aerugenosis and C. albicans biofilm destruction. The conducted studies prove the feasibility of further study of the combined gel with the phyto complex № 4 containing dry walnut leaf extract with the sum of tannins in terms of gallic acid and dry matter 30 mg/100 g of gel, dry nettle extract with the sum of hydroxycinnamic acids in terms of chlorogenic acid, and dry matter 20 mg/100 g of gel, dry thyme extract with the sum of flavonoids in terms of rutin and dry matter 35 mg/100 g of gel.V. S. MyrhorodN. I. FilimonovaO. G. BashuraS. G. BobroThe State Expert Center of the Ministry of Health of Ukrainearticlebiofilmsdry walnut leaf extractdry nettle extractdry thyme extractgelTherapeutics. PharmacologyRM1-950Pharmacy and materia medicaRS1-441UKФармацевтичний журнал, Vol 5, Pp 68-76 (2021)
institution	DOAJ
collection	DOAJ
language	UK
topic	biofilms dry walnut leaf extract dry nettle extract dry thyme extract gel Therapeutics. Pharmacology RM1-950 Pharmacy and materia medica RS1-441
spellingShingle	biofilms dry walnut leaf extract dry nettle extract dry thyme extract gel Therapeutics. Pharmacology RM1-950 Pharmacy and materia medica RS1-441 V. S. Myrhorod N. I. Filimonova O. G. Bashura S. G. Bobro Study of the effect of dermatological phytogel on the ability of microorganisms to form a biofilm
description	The development of many chronic infections, including skin diseases, is caused by bacteria growing in the form of biofilms. Bacterial biofilms provide beneficial survival mechanisms that determine virulence, disease pathogenesis, or resistance of the pathogen to antibiotics. As shown by a large number of studies, biofilms play an important role in the pathogenesis of dermatological diseases, including atopic dermatitis. The close relationship between the microbial biofilm that colonizes the skin surface and the negative consequences for human health makes the skin microbiome an object of therapeutic intervention in dermatological pathogenic processes. The work aims to study the effect of dermatological phytogel on the ability of microorganisms to form biofilms. The objects of research were samples of gel containing dry walnut leaf extract with the sum of tannins in terms of gallic acid and dry matter 30 mg/100 g of gel, dry nettle extract with the sum of hydroxycinnamic acids in terms of chlorogenic acid, and dry matter 20 mg/100 g of gel, dry thyme extract with the sum of flavonoids in terms of rutin and dry matter 35 mg/100 g of gel both monocomponent and combined. The study of the ability of individual plant components of phytogel samples N 1, N 2, N 3 and samples of combined phytogel N 4, N 5, and N 6 to influence biofilm formation have shown that the most pronounced decceleration of biofilms formation was registered in the gel sample with phytocomplex N 4 and was 19.7–20.7% to S. aureus, E. coli, P. aerugenosis and C. albicans respectively. The activity of the gel sample with phytocomplex N 4 was 1.3–1.4 times higher than that of monocomponent gel samples N 1, N 2, and N 3. When determining the ability of the test samples to destroy biofilms, it has been found that the gel sample with phytocomplex N 4 showed the greatest activity, which exceeded the specified properties of samples N 5 and N 6 by an average of 1.2 and 1.8 times. The activity of single-component gel samples N 1, N 2, and N 3 was lower in S. aureus, E. coli, P. aerugenosis and C. albicans biofilm destruction. The conducted studies prove the feasibility of further study of the combined gel with the phyto complex № 4 containing dry walnut leaf extract with the sum of tannins in terms of gallic acid and dry matter 30 mg/100 g of gel, dry nettle extract with the sum of hydroxycinnamic acids in terms of chlorogenic acid, and dry matter 20 mg/100 g of gel, dry thyme extract with the sum of flavonoids in terms of rutin and dry matter 35 mg/100 g of gel.
format	article
author	V. S. Myrhorod N. I. Filimonova O. G. Bashura S. G. Bobro
author_facet	V. S. Myrhorod N. I. Filimonova O. G. Bashura S. G. Bobro
author_sort	V. S. Myrhorod
title	Study of the effect of dermatological phytogel on the ability of microorganisms to form a biofilm
title_short	Study of the effect of dermatological phytogel on the ability of microorganisms to form a biofilm
title_full	Study of the effect of dermatological phytogel on the ability of microorganisms to form a biofilm
title_fullStr	Study of the effect of dermatological phytogel on the ability of microorganisms to form a biofilm
title_full_unstemmed	Study of the effect of dermatological phytogel on the ability of microorganisms to form a biofilm
title_sort	study of the effect of dermatological phytogel on the ability of microorganisms to form a biofilm
publisher	The State Expert Center of the Ministry of Health of Ukraine
publishDate	2021
url	https://doaj.org/article/36cc6c0a8b794a13a6e68b6ffa72e5ba
work_keys_str_mv	AT vsmyrhorod studyoftheeffectofdermatologicalphytogelontheabilityofmicroorganismstoformabiofilm AT nifilimonova studyoftheeffectofdermatologicalphytogelontheabilityofmicroorganismstoformabiofilm AT ogbashura studyoftheeffectofdermatologicalphytogelontheabilityofmicroorganismstoformabiofilm AT sgbobro studyoftheeffectofdermatologicalphytogelontheabilityofmicroorganismstoformabiofilm
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Study of the effect of dermatological phytogel on the ability of microorganisms to form a biofilm

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