Development and regeneration of somatic embryos from leaves-derived calli of Coffea liberica

Abstract. Ardiyani F, Utami ESW, Purnobasuki H, Paramita SA. 2020. Development and regeneration of somatic embryos from leaves-derived calli of Coffea liberica. Biodiversitas 21: 5829-5834. Coffea liberica is an important and potentially commercial plant with a high economic value from the Coffea ge...

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Autores principales:	FITRIA ARDIYANI, Edy Setiti Wida Utami, HERY PURNOBASUKI, SENJA APRILIA PARAMITA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	coffea liberica, plant growth regulators, regeneration, somatic embryo Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/36db193a6ecb4f3f9ffcbe30222de020
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spelling	oai:doaj.org-article:36db193a6ecb4f3f9ffcbe30222de0202021-11-22T00:52:00ZDevelopment and regeneration of somatic embryos from leaves-derived calli of Coffea liberica1412-033X2085-472210.13057/biodiv/d211246https://doaj.org/article/36db193a6ecb4f3f9ffcbe30222de0202020-11-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6940https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Ardiyani F, Utami ESW, Purnobasuki H, Paramita SA. 2020. Development and regeneration of somatic embryos from leaves-derived calli of Coffea liberica. Biodiversitas 21: 5829-5834. Coffea liberica is an important and potentially commercial plant with a high economic value from the Coffea genus. Therefore, the availability of planting material is needed to increase productivity and ensure the sustainability of its farming. Somatic embryogenesis is a powerful propagation method used to produce clonal plants from limited genetic material. In the present research, we have shown that C. liberica could be successfully regenerated in vitro via somatic embryogenesis from leaves derived embryogenic callus. These calli were cultured on Murashige Skoog (MS) medium added with 1 mgL-1 BAP or in combination with 2.4 D (0.5, 1.0, 1.5 and 2 mgL-1) for embryo development induction. Furthermore, the medium containing only BAP was best for embryo development induction after culturing for 12 weeks, with the highest number of cotyledonary stage embryos (17.8%) and producing a total of embryo (20.2). Following cotyledonary stage embryo were cultured on new MS medium containing 0.5 mgL-1 BAP, 0.5 mgL-1 IAA, 0.5 mgL-1 NAA only, and 0.5 mgL-1 BAP in combination with 0.5 mgL-1 IAA or 0.5 mgL-1 NAA. Interestingly, the results showed that cotyledonary stage embryos were converted into complete plants at all treatment, but the MS medium containing 0.5 mgL-1 BAP was found to be the most effective in promoting regeneration with 2.6 leaves per-plantlet and height of 5.2 mm. Based morphological analysis confirm that the development of somatic embryo from leaves-derived calli of Coffea liberica started with the formation of embryo globular, heart, torpedo, cotyledonary stages, and finally conversion of cotyledonary embryo into complete plant.FITRIA ARDIYANIEdy Setiti Wida UtamiHERY PURNOBASUKISENJA APRILIA PARAMITAMBI & UNS Soloarticlecoffea liberica, plant growth regulators, regeneration, somatic embryoBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	coffea liberica, plant growth regulators, regeneration, somatic embryo Biology (General) QH301-705.5
spellingShingle	coffea liberica, plant growth regulators, regeneration, somatic embryo Biology (General) QH301-705.5 FITRIA ARDIYANI Edy Setiti Wida Utami HERY PURNOBASUKI SENJA APRILIA PARAMITA Development and regeneration of somatic embryos from leaves-derived calli of Coffea liberica
description	Abstract. Ardiyani F, Utami ESW, Purnobasuki H, Paramita SA. 2020. Development and regeneration of somatic embryos from leaves-derived calli of Coffea liberica. Biodiversitas 21: 5829-5834. Coffea liberica is an important and potentially commercial plant with a high economic value from the Coffea genus. Therefore, the availability of planting material is needed to increase productivity and ensure the sustainability of its farming. Somatic embryogenesis is a powerful propagation method used to produce clonal plants from limited genetic material. In the present research, we have shown that C. liberica could be successfully regenerated in vitro via somatic embryogenesis from leaves derived embryogenic callus. These calli were cultured on Murashige Skoog (MS) medium added with 1 mgL-1 BAP or in combination with 2.4 D (0.5, 1.0, 1.5 and 2 mgL-1) for embryo development induction. Furthermore, the medium containing only BAP was best for embryo development induction after culturing for 12 weeks, with the highest number of cotyledonary stage embryos (17.8%) and producing a total of embryo (20.2). Following cotyledonary stage embryo were cultured on new MS medium containing 0.5 mgL-1 BAP, 0.5 mgL-1 IAA, 0.5 mgL-1 NAA only, and 0.5 mgL-1 BAP in combination with 0.5 mgL-1 IAA or 0.5 mgL-1 NAA. Interestingly, the results showed that cotyledonary stage embryos were converted into complete plants at all treatment, but the MS medium containing 0.5 mgL-1 BAP was found to be the most effective in promoting regeneration with 2.6 leaves per-plantlet and height of 5.2 mm. Based morphological analysis confirm that the development of somatic embryo from leaves-derived calli of Coffea liberica started with the formation of embryo globular, heart, torpedo, cotyledonary stages, and finally conversion of cotyledonary embryo into complete plant.
format	article
author	FITRIA ARDIYANI Edy Setiti Wida Utami HERY PURNOBASUKI SENJA APRILIA PARAMITA
author_facet	FITRIA ARDIYANI Edy Setiti Wida Utami HERY PURNOBASUKI SENJA APRILIA PARAMITA
author_sort	FITRIA ARDIYANI
title	Development and regeneration of somatic embryos from leaves-derived calli of Coffea liberica
title_short	Development and regeneration of somatic embryos from leaves-derived calli of Coffea liberica
title_full	Development and regeneration of somatic embryos from leaves-derived calli of Coffea liberica
title_fullStr	Development and regeneration of somatic embryos from leaves-derived calli of Coffea liberica
title_full_unstemmed	Development and regeneration of somatic embryos from leaves-derived calli of Coffea liberica
title_sort	development and regeneration of somatic embryos from leaves-derived calli of coffea liberica
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/36db193a6ecb4f3f9ffcbe30222de020
work_keys_str_mv	AT fitriaardiyani developmentandregenerationofsomaticembryosfromleavesderivedcalliofcoffealiberica AT edysetitiwidautami developmentandregenerationofsomaticembryosfromleavesderivedcalliofcoffealiberica AT herypurnobasuki developmentandregenerationofsomaticembryosfromleavesderivedcalliofcoffealiberica AT senjaapriliaparamita developmentandregenerationofsomaticembryosfromleavesderivedcalliofcoffealiberica
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Development and regeneration of somatic embryos from leaves-derived calli of Coffea liberica

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