Identification and diversity analysis of cassiicolin-encoding gene of Corynespora cassiicola isolates from rubber tree in Indonesia

Abstract. Oktavia F. 2020. Identification and diversity analysis of cassiicolin encoding gene of Corynespora cassiicola isolates from rubber tree in Indonesia. Biodiversitas 21: 3499-3507. Cassiicolin is an important effector of virulence process on the Corynespora cassiicola, plant-pathogen that ca...

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Autor principal:	FETRINA OKTAVIA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	cas gene, corynespora leaf fall disease, hevea brasiliensis, pathogen effector, toxin Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/36e41e180ab6419ea3968851dc21dac4
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spelling	oai:doaj.org-article:36e41e180ab6419ea3968851dc21dac42021-11-22T12:17:57ZIdentification and diversity analysis of cassiicolin-encoding gene of Corynespora cassiicola isolates from rubber tree in Indonesia1412-033X2085-472210.13057/biodiv/d210811https://doaj.org/article/36e41e180ab6419ea3968851dc21dac42020-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5939https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Oktavia F. 2020. Identification and diversity analysis of cassiicolin encoding gene of Corynespora cassiicola isolates from rubber tree in Indonesia. Biodiversitas 21: 3499-3507. Cassiicolin is an important effector of virulence process on the Corynespora cassiicola, plant-pathogen that causes Corynespora leaf fall disease in rubber tree. The study aimed to identify and analyze the genetic diversity of isolates from rubber plants in Indonesian based on cassiicolin encoding gene (Cas gene). Identification of Cas genes was performed using 12 pairs of primers on 23 isolates from rubber host clones. Only three pairs of primers were able to amplify the analyzed DNA, namely CasF20/CasR28, CasF19/CasR26, and CasF4/R16 which produced DNA fragments of 760 bp, 550 bp and 210 bp respectively in this study. BLAST analysis showed that 19 isolates carried a Cas5 gene. Three of the 19 DNA samples also contained a putative Cas3 or Cas4 gene. Four other isolates had no detectable Cas gene (Cas0). The genetic diversity based on the Cas gene showed that the isolates were grouped into three major clusters. ome isolates had high genetic similarity with outgroup isolates from China. There was not clear link between Cas gene with virulence profiles. The cassiicolin encoding gene information is useful for predicting the virulence of C. cassiicola isolates from Indonesia rubber plantation, so that the disease severity spreading in rubber plants can be early anticipated.FETRINA OKTAVIAMBI & UNS Soloarticlecas gene, corynespora leaf fall disease, hevea brasiliensis, pathogen effector, toxinBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	cas gene, corynespora leaf fall disease, hevea brasiliensis, pathogen effector, toxin Biology (General) QH301-705.5
spellingShingle	cas gene, corynespora leaf fall disease, hevea brasiliensis, pathogen effector, toxin Biology (General) QH301-705.5 FETRINA OKTAVIA Identification and diversity analysis of cassiicolin-encoding gene of Corynespora cassiicola isolates from rubber tree in Indonesia
description	Abstract. Oktavia F. 2020. Identification and diversity analysis of cassiicolin encoding gene of Corynespora cassiicola isolates from rubber tree in Indonesia. Biodiversitas 21: 3499-3507. Cassiicolin is an important effector of virulence process on the Corynespora cassiicola, plant-pathogen that causes Corynespora leaf fall disease in rubber tree. The study aimed to identify and analyze the genetic diversity of isolates from rubber plants in Indonesian based on cassiicolin encoding gene (Cas gene). Identification of Cas genes was performed using 12 pairs of primers on 23 isolates from rubber host clones. Only three pairs of primers were able to amplify the analyzed DNA, namely CasF20/CasR28, CasF19/CasR26, and CasF4/R16 which produced DNA fragments of 760 bp, 550 bp and 210 bp respectively in this study. BLAST analysis showed that 19 isolates carried a Cas5 gene. Three of the 19 DNA samples also contained a putative Cas3 or Cas4 gene. Four other isolates had no detectable Cas gene (Cas0). The genetic diversity based on the Cas gene showed that the isolates were grouped into three major clusters. ome isolates had high genetic similarity with outgroup isolates from China. There was not clear link between Cas gene with virulence profiles. The cassiicolin encoding gene information is useful for predicting the virulence of C. cassiicola isolates from Indonesia rubber plantation, so that the disease severity spreading in rubber plants can be early anticipated.
format	article
author	FETRINA OKTAVIA
author_facet	FETRINA OKTAVIA
author_sort	FETRINA OKTAVIA
title	Identification and diversity analysis of cassiicolin-encoding gene of Corynespora cassiicola isolates from rubber tree in Indonesia
title_short	Identification and diversity analysis of cassiicolin-encoding gene of Corynespora cassiicola isolates from rubber tree in Indonesia
title_full	Identification and diversity analysis of cassiicolin-encoding gene of Corynespora cassiicola isolates from rubber tree in Indonesia
title_fullStr	Identification and diversity analysis of cassiicolin-encoding gene of Corynespora cassiicola isolates from rubber tree in Indonesia
title_full_unstemmed	Identification and diversity analysis of cassiicolin-encoding gene of Corynespora cassiicola isolates from rubber tree in Indonesia
title_sort	identification and diversity analysis of cassiicolin-encoding gene of corynespora cassiicola isolates from rubber tree in indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/36e41e180ab6419ea3968851dc21dac4
work_keys_str_mv	AT fetrinaoktavia identificationanddiversityanalysisofcassiicolinencodinggeneofcorynesporacassiicolaisolatesfromrubbertreeinindonesia
_version_	1718417668474667008

Identification and diversity analysis of cassiicolin-encoding gene of Corynespora cassiicola isolates from rubber tree in Indonesia

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