MicroRNA profiling in human neutrophils during bone marrow granulopoiesis and in vivo exudation.

The purpose of this study was to describe the microRNA (miRNA) expression profiles of neutrophils and their precursors from the initiation of granulopoiesis in the bone marrow to extravasation and accumulation in skin windows. We analyzed three different cell populations from human bone marrow, poly...

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Autores principales:	Maria T Larsen, Christoffer Hother, Mattias Häger, Corinna C Pedersen, Kim Theilgaard-Mönch, Niels Borregaard, Jack B Cowland
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/36eac848033041eaa9f8ddf61fe23bf5
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spelling	oai:doaj.org-article:36eac848033041eaa9f8ddf61fe23bf52021-11-18T07:53:50ZMicroRNA profiling in human neutrophils during bone marrow granulopoiesis and in vivo exudation.1932-620310.1371/journal.pone.0058454https://doaj.org/article/36eac848033041eaa9f8ddf61fe23bf52013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23554893/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The purpose of this study was to describe the microRNA (miRNA) expression profiles of neutrophils and their precursors from the initiation of granulopoiesis in the bone marrow to extravasation and accumulation in skin windows. We analyzed three different cell populations from human bone marrow, polymorphonuclear neutrophil (PMNs) from peripheral blood, and extravasated PMNs from skin windows using the Affymetrix 2.0 platform. Our data reveal 135 miRNAs differentially regulated during bone marrow granulopoiesis. The majority is differentially regulated between the myeloblast/promyelocyte (MB/PM) and myelocyte/metamyelocyte (MC/MM) stages of development. These 135 miRNAs were divided into six clusters according to the pattern of their expression. Several miRNAs demonstrate a pronounced increase or reduction at the transition between MB/PM and MC/MM, which is associated with cell cycle arrest and the initiation of terminal differentiation. Seven miRNAs are differentially up-regulated between peripheral blood PMNs and extravasated PMNs and only one of these (miR-132) is also differentially regulated during granulopoiesis. The study indicates that several different miRNAs participate in the regulation of normal granulopoiesis and that miRNAs might also regulate activities of extravasated neutrophils. The data present the miRNA profiles during the development and activation of the neutrophil granulocyte in healthy humans and thus serves as a reference for further research of normal and malignant granulocytic development.Maria T LarsenChristoffer HotherMattias HägerCorinna C PedersenKim Theilgaard-MönchNiels BorregaardJack B CowlandPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58454 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria T Larsen Christoffer Hother Mattias Häger Corinna C Pedersen Kim Theilgaard-Mönch Niels Borregaard Jack B Cowland MicroRNA profiling in human neutrophils during bone marrow granulopoiesis and in vivo exudation.
description	The purpose of this study was to describe the microRNA (miRNA) expression profiles of neutrophils and their precursors from the initiation of granulopoiesis in the bone marrow to extravasation and accumulation in skin windows. We analyzed three different cell populations from human bone marrow, polymorphonuclear neutrophil (PMNs) from peripheral blood, and extravasated PMNs from skin windows using the Affymetrix 2.0 platform. Our data reveal 135 miRNAs differentially regulated during bone marrow granulopoiesis. The majority is differentially regulated between the myeloblast/promyelocyte (MB/PM) and myelocyte/metamyelocyte (MC/MM) stages of development. These 135 miRNAs were divided into six clusters according to the pattern of their expression. Several miRNAs demonstrate a pronounced increase or reduction at the transition between MB/PM and MC/MM, which is associated with cell cycle arrest and the initiation of terminal differentiation. Seven miRNAs are differentially up-regulated between peripheral blood PMNs and extravasated PMNs and only one of these (miR-132) is also differentially regulated during granulopoiesis. The study indicates that several different miRNAs participate in the regulation of normal granulopoiesis and that miRNAs might also regulate activities of extravasated neutrophils. The data present the miRNA profiles during the development and activation of the neutrophil granulocyte in healthy humans and thus serves as a reference for further research of normal and malignant granulocytic development.
format	article
author	Maria T Larsen Christoffer Hother Mattias Häger Corinna C Pedersen Kim Theilgaard-Mönch Niels Borregaard Jack B Cowland
author_facet	Maria T Larsen Christoffer Hother Mattias Häger Corinna C Pedersen Kim Theilgaard-Mönch Niels Borregaard Jack B Cowland
author_sort	Maria T Larsen
title	MicroRNA profiling in human neutrophils during bone marrow granulopoiesis and in vivo exudation.
title_short	MicroRNA profiling in human neutrophils during bone marrow granulopoiesis and in vivo exudation.
title_full	MicroRNA profiling in human neutrophils during bone marrow granulopoiesis and in vivo exudation.
title_fullStr	MicroRNA profiling in human neutrophils during bone marrow granulopoiesis and in vivo exudation.
title_full_unstemmed	MicroRNA profiling in human neutrophils during bone marrow granulopoiesis and in vivo exudation.
title_sort	microrna profiling in human neutrophils during bone marrow granulopoiesis and in vivo exudation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/36eac848033041eaa9f8ddf61fe23bf5
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_version_	1718422777911836672

MicroRNA profiling in human neutrophils during bone marrow granulopoiesis and in vivo exudation.

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