Fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy

Huei-Ling Huang,1 Pei-Hsuan Lu,1 Hung-Chih Yang,1 Gi-Da Lee,1,2 Han-Ru Li,1 Kuo-Chih Liao1 1Graduate Institute of Biomedical Engineering, National Chung Hsing University, 2Department of Radiology, Taichung Veterans General Hospital, Taichung, Taiwan Abstract: The aim of this research is t...

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Autores principales:	Huang HL, Lu PH, Yang HC, Lee GD, Li HR, Liao KC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/36eaf09ac45f40cb8651bfbb69a1ab35
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spelling	oai:doaj.org-article:36eaf09ac45f40cb8651bfbb69a1ab352021-12-02T02:56:53ZFiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy1178-2013https://doaj.org/article/36eaf09ac45f40cb8651bfbb69a1ab352015-08-01T00:00:00Zhttp://www.dovepress.com/fiber-optic-triggered-release-of-liposome-in-vivo-implication-of-perso-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Huei-Ling Huang,1 Pei-Hsuan Lu,1 Hung-Chih Yang,1 Gi-Da Lee,1,2 Han-Ru Li,1 Kuo-Chih Liao1 1Graduate Institute of Biomedical Engineering, National Chung Hsing University, 2Department of Radiology, Taichung Veterans General Hospital, Taichung, Taiwan Abstract: The aim of this research is to provide proof of principle by applying the fiber-optic triggered release of photo-thermally responsive liposomes embedded with gold nanoparticles (AuNPs) using a 200 µm fiber with 65 mW and 532 nm excitation for topical release in vivo. The tunable delivery function can be paired with an apoptosis biosensor based on the same fiber-optic configuration for providing real-time evaluation of chemotherapy efficacy in vivo to perform as a personalized chemotherapy system. The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes. In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements. Using the mouse xenograft studies, we first demonstrated that the encapsulation of fluorescein in liposomes resulted in a more substantial content retention (81%) in the tumor than for free fluorophores (14%) at 120 minutes after administration from in vivo fluorescence imaging. Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation. Keywords: fiber-optic guided excitation, light excitation triggered release, photo-thermal responsive liposome, gold nanoparticles, tunable release in vivoHuang HLLu PHYang HCLee GDLi HRLiao KCDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 5171-5185 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Huang HL Lu PH Yang HC Lee GD Li HR Liao KC Fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy
description	Huei-Ling Huang,1 Pei-Hsuan Lu,1 Hung-Chih Yang,1 Gi-Da Lee,1,2 Han-Ru Li,1 Kuo-Chih Liao1 1Graduate Institute of Biomedical Engineering, National Chung Hsing University, 2Department of Radiology, Taichung Veterans General Hospital, Taichung, Taiwan Abstract: The aim of this research is to provide proof of principle by applying the fiber-optic triggered release of photo-thermally responsive liposomes embedded with gold nanoparticles (AuNPs) using a 200 µm fiber with 65 mW and 532 nm excitation for topical release in vivo. The tunable delivery function can be paired with an apoptosis biosensor based on the same fiber-optic configuration for providing real-time evaluation of chemotherapy efficacy in vivo to perform as a personalized chemotherapy system. The pattern of topical release triggered by laser excitation conveyed through optical fibers was monitored by the increase in fluorescence resulting from the dilution of self-quenching (75 mM) fluorescein encapsulated in liposomes. In in vitro studies (in 37°C phosphate buffer saline), the AuNP-embedded liposomes showed a more efficient triggered release (74.53%±1.63% in 40 minutes) than traditional temperature-responsive liposomes without AuNPs (14.53%±3.17%) or AuNP-liposomes without excitation (21.92%±2.08%) by spectroscopic measurements. Using the mouse xenograft studies, we first demonstrated that the encapsulation of fluorescein in liposomes resulted in a more substantial content retention (81%) in the tumor than for free fluorophores (14%) at 120 minutes after administration from in vivo fluorescence imaging. Furthermore, the preliminary results also suggested the tunable release capability of the system by demonstrating consecutive triggered releases with fiber-optic guided laser excitation. Keywords: fiber-optic guided excitation, light excitation triggered release, photo-thermal responsive liposome, gold nanoparticles, tunable release in vivo
format	article
author	Huang HL Lu PH Yang HC Lee GD Li HR Liao KC
author_facet	Huang HL Lu PH Yang HC Lee GD Li HR Liao KC
author_sort	Huang HL
title	Fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy
title_short	Fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy
title_full	Fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy
title_fullStr	Fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy
title_full_unstemmed	Fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy
title_sort	fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/36eaf09ac45f40cb8651bfbb69a1ab35
work_keys_str_mv	AT huanghl fiberoptictriggeredreleaseofliposomeinvivoimplicationofpersonalizedchemotherapy AT luph fiberoptictriggeredreleaseofliposomeinvivoimplicationofpersonalizedchemotherapy AT yanghc fiberoptictriggeredreleaseofliposomeinvivoimplicationofpersonalizedchemotherapy AT leegd fiberoptictriggeredreleaseofliposomeinvivoimplicationofpersonalizedchemotherapy AT lihr fiberoptictriggeredreleaseofliposomeinvivoimplicationofpersonalizedchemotherapy AT liaokc fiberoptictriggeredreleaseofliposomeinvivoimplicationofpersonalizedchemotherapy
_version_	1718402032869572608

Fiber-optic triggered release of liposome in vivo: implication of personalized chemotherapy

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