Dietary carbohydrates influence muscle texture of olive flounder Paralichthys olivaceus through impacting mitochondria function and metabolism of glycogen and protein

Abstract The present study was conducted to estimate the effects of dietary carbohydrates on muscle quality and the underlying mechanisms. Six isonitrogenous and isolipidic diets were formulated to contain graded levels of carbohydrates (0%, 8%, 12%, 16%, 20% and 24%, respectively). These diets were...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jiahuan Liu, Kangyu Deng, Mingzhu Pan, Guangxia Liu, Jing Wu, Mengxi Yang, Dong Huang, Wenbing Zhang, Kangsen Mai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/36eda7333c1c459e89f91b2aeac46b28
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:36eda7333c1c459e89f91b2aeac46b28
record_format	dspace
spelling	oai:doaj.org-article:36eda7333c1c459e89f91b2aeac46b282021-12-02T12:33:05ZDietary carbohydrates influence muscle texture of olive flounder Paralichthys olivaceus through impacting mitochondria function and metabolism of glycogen and protein10.1038/s41598-020-76255-32045-2322https://doaj.org/article/36eda7333c1c459e89f91b2aeac46b282020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-76255-3https://doaj.org/toc/2045-2322Abstract The present study was conducted to estimate the effects of dietary carbohydrates on muscle quality and the underlying mechanisms. Six isonitrogenous and isolipidic diets were formulated to contain graded levels of carbohydrates (0%, 8%, 12%, 16%, 20% and 24%, respectively). These diets were named as C0, C8, C12, C16, C20 and C24, respectively. After a 10-week feeding trial, results showed that the muscle pH, liquid holding capacity (LHC) and hardness were significantly decreased by the increasing dietary carbohydrate levels. Dietary carbohydrates significantly decreased the muscle fibre diameter, and the highest value was found in the C0 group. Accumulated glycogen and degenerated mitochondrial cristae were observed in the C24 group. Significantly higher contents of protein carbonyls were observed in the C20 group and C24 group (P < 0.05). There was a significant decrease of mtDNA copy number in the C24 group compared with that in the C0 and C8 groups. The AMP/ATP ratio in muscle decreased first and then increased with the increasing dietary carbohydrate levels. The dietary incorporation of carbohydrate significantly reduced the expression of opa1, pygm and genes involved in myogenesis (myf5 and myog). Meanwhile, proteolysis-related genes (murf-1, mafbx, capn2 and ctsl), pro-inflammatory cytokines (il-6 and tnf-α) and mstn were significantly up-regulated. In the C24 group, significant increase of phosphorylation of AMPK (Thr172), up-regulation of PGC-1α and GLUT4 were observed, while the phosphorylation level of S6 (Ser235/236) was significantly decreased. It was concluded that excessive dietary carbohydrate level (24%) had negative impacts on mitochondria function and promoted glycogen accumulation, and thereafter influenced the muscle quality of olive flounder. The activation of AMPK as well as the upregulation of PGC-1α and GLUT4 was the key mechanism.Jiahuan LiuKangyu DengMingzhu PanGuangxia LiuJing WuMengxi YangDong HuangWenbing ZhangKangsen MaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-20 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jiahuan Liu Kangyu Deng Mingzhu Pan Guangxia Liu Jing Wu Mengxi Yang Dong Huang Wenbing Zhang Kangsen Mai Dietary carbohydrates influence muscle texture of olive flounder Paralichthys olivaceus through impacting mitochondria function and metabolism of glycogen and protein
description	Abstract The present study was conducted to estimate the effects of dietary carbohydrates on muscle quality and the underlying mechanisms. Six isonitrogenous and isolipidic diets were formulated to contain graded levels of carbohydrates (0%, 8%, 12%, 16%, 20% and 24%, respectively). These diets were named as C0, C8, C12, C16, C20 and C24, respectively. After a 10-week feeding trial, results showed that the muscle pH, liquid holding capacity (LHC) and hardness were significantly decreased by the increasing dietary carbohydrate levels. Dietary carbohydrates significantly decreased the muscle fibre diameter, and the highest value was found in the C0 group. Accumulated glycogen and degenerated mitochondrial cristae were observed in the C24 group. Significantly higher contents of protein carbonyls were observed in the C20 group and C24 group (P < 0.05). There was a significant decrease of mtDNA copy number in the C24 group compared with that in the C0 and C8 groups. The AMP/ATP ratio in muscle decreased first and then increased with the increasing dietary carbohydrate levels. The dietary incorporation of carbohydrate significantly reduced the expression of opa1, pygm and genes involved in myogenesis (myf5 and myog). Meanwhile, proteolysis-related genes (murf-1, mafbx, capn2 and ctsl), pro-inflammatory cytokines (il-6 and tnf-α) and mstn were significantly up-regulated. In the C24 group, significant increase of phosphorylation of AMPK (Thr172), up-regulation of PGC-1α and GLUT4 were observed, while the phosphorylation level of S6 (Ser235/236) was significantly decreased. It was concluded that excessive dietary carbohydrate level (24%) had negative impacts on mitochondria function and promoted glycogen accumulation, and thereafter influenced the muscle quality of olive flounder. The activation of AMPK as well as the upregulation of PGC-1α and GLUT4 was the key mechanism.
format	article
author	Jiahuan Liu Kangyu Deng Mingzhu Pan Guangxia Liu Jing Wu Mengxi Yang Dong Huang Wenbing Zhang Kangsen Mai
author_facet	Jiahuan Liu Kangyu Deng Mingzhu Pan Guangxia Liu Jing Wu Mengxi Yang Dong Huang Wenbing Zhang Kangsen Mai
author_sort	Jiahuan Liu
title	Dietary carbohydrates influence muscle texture of olive flounder Paralichthys olivaceus through impacting mitochondria function and metabolism of glycogen and protein
title_short	Dietary carbohydrates influence muscle texture of olive flounder Paralichthys olivaceus through impacting mitochondria function and metabolism of glycogen and protein
title_full	Dietary carbohydrates influence muscle texture of olive flounder Paralichthys olivaceus through impacting mitochondria function and metabolism of glycogen and protein
title_fullStr	Dietary carbohydrates influence muscle texture of olive flounder Paralichthys olivaceus through impacting mitochondria function and metabolism of glycogen and protein
title_full_unstemmed	Dietary carbohydrates influence muscle texture of olive flounder Paralichthys olivaceus through impacting mitochondria function and metabolism of glycogen and protein
title_sort	dietary carbohydrates influence muscle texture of olive flounder paralichthys olivaceus through impacting mitochondria function and metabolism of glycogen and protein
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/36eda7333c1c459e89f91b2aeac46b28
work_keys_str_mv	AT jiahuanliu dietarycarbohydratesinfluencemuscletextureofoliveflounderparalichthysolivaceusthroughimpactingmitochondriafunctionandmetabolismofglycogenandprotein AT kangyudeng dietarycarbohydratesinfluencemuscletextureofoliveflounderparalichthysolivaceusthroughimpactingmitochondriafunctionandmetabolismofglycogenandprotein AT mingzhupan dietarycarbohydratesinfluencemuscletextureofoliveflounderparalichthysolivaceusthroughimpactingmitochondriafunctionandmetabolismofglycogenandprotein AT guangxialiu dietarycarbohydratesinfluencemuscletextureofoliveflounderparalichthysolivaceusthroughimpactingmitochondriafunctionandmetabolismofglycogenandprotein AT jingwu dietarycarbohydratesinfluencemuscletextureofoliveflounderparalichthysolivaceusthroughimpactingmitochondriafunctionandmetabolismofglycogenandprotein AT mengxiyang dietarycarbohydratesinfluencemuscletextureofoliveflounderparalichthysolivaceusthroughimpactingmitochondriafunctionandmetabolismofglycogenandprotein AT donghuang dietarycarbohydratesinfluencemuscletextureofoliveflounderparalichthysolivaceusthroughimpactingmitochondriafunctionandmetabolismofglycogenandprotein AT wenbingzhang dietarycarbohydratesinfluencemuscletextureofoliveflounderparalichthysolivaceusthroughimpactingmitochondriafunctionandmetabolismofglycogenandprotein AT kangsenmai dietarycarbohydratesinfluencemuscletextureofoliveflounderparalichthysolivaceusthroughimpactingmitochondriafunctionandmetabolismofglycogenandprotein
_version_	1718393851798880256

Dietary carbohydrates influence muscle texture of olive flounder Paralichthys olivaceus through impacting mitochondria function and metabolism of glycogen and protein

Ejemplares similares