Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA
Wang et al. show how genetic sequence and base modifications can be detected simultaneously on single molecules of both DNA and RNA using magnetic tweezers. They also demonstrate an amplification-free CRISPR/Cas9-based strategy for isolating target regions from native DNA and apply this approach to...
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Nature Portfolio
2021
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oai:doaj.org-article:36f2d6c25b90460cab0986d3713e402b2021-12-02T13:24:17ZDetection of genetic variation and base modifications at base-pair resolution on both DNA and RNA10.1038/s42003-021-01648-72399-3642https://doaj.org/article/36f2d6c25b90460cab0986d3713e402b2021-01-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01648-7https://doaj.org/toc/2399-3642Wang et al. show how genetic sequence and base modifications can be detected simultaneously on single molecules of both DNA and RNA using magnetic tweezers. They also demonstrate an amplification-free CRISPR/Cas9-based strategy for isolating target regions from native DNA and apply this approach to the isolation of targets from E. coli and human genomic DNA.Zhen WangJérôme MaluendaLaurène GirautThibault VieilleAndréas LefevreDavid SalthouseGaël RadouRémi MoulinasSandra AstetePol D’AvezacGeoff SmithCharles AndréJean-François AllemandDavid BensimonVincent CroquetteJimmy OuelletGordon HamiltonNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-14 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Biology (General) QH301-705.5 |
| spellingShingle |
Biology (General) QH301-705.5 Zhen Wang Jérôme Maluenda Laurène Giraut Thibault Vieille Andréas Lefevre David Salthouse Gaël Radou Rémi Moulinas Sandra Astete Pol D’Avezac Geoff Smith Charles André Jean-François Allemand David Bensimon Vincent Croquette Jimmy Ouellet Gordon Hamilton Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA |
| description |
Wang et al. show how genetic sequence and base modifications can be detected simultaneously on single molecules of both DNA and RNA using magnetic tweezers. They also demonstrate an amplification-free CRISPR/Cas9-based strategy for isolating target regions from native DNA and apply this approach to the isolation of targets from E. coli and human genomic DNA. |
| format |
article |
| author |
Zhen Wang Jérôme Maluenda Laurène Giraut Thibault Vieille Andréas Lefevre David Salthouse Gaël Radou Rémi Moulinas Sandra Astete Pol D’Avezac Geoff Smith Charles André Jean-François Allemand David Bensimon Vincent Croquette Jimmy Ouellet Gordon Hamilton |
| author_facet |
Zhen Wang Jérôme Maluenda Laurène Giraut Thibault Vieille Andréas Lefevre David Salthouse Gaël Radou Rémi Moulinas Sandra Astete Pol D’Avezac Geoff Smith Charles André Jean-François Allemand David Bensimon Vincent Croquette Jimmy Ouellet Gordon Hamilton |
| author_sort |
Zhen Wang |
| title |
Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA |
| title_short |
Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA |
| title_full |
Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA |
| title_fullStr |
Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA |
| title_full_unstemmed |
Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA |
| title_sort |
detection of genetic variation and base modifications at base-pair resolution on both dna and rna |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/36f2d6c25b90460cab0986d3713e402b |
| work_keys_str_mv |
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