Application of TaqMan Real-Time PCR for Detecting ‘<i>Candidatus</i> Arsenophonus Phytopathogenicus’ Infection in Sugar Beet

The γ-proteobacterium ‘<i>Candidatus</i> Arsenophonus phytopathogenicus’ is assigned as the major pathogen of “Syndrome des basses richesses”, a sugar beet disease characterised by a reduction in the sugar content of taproots and biomass yield. Despite the economic impact of this bacteri...

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Autores principales:	Christina Zübert, Michael Kube
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	TaqMan qPCR ‘<i>Candidatus</i> Arsenophonus phytopathogenicus’ SBR sugar beet Medicine R
Acceso en línea:	https://doaj.org/article/36f501c2bdcd4637b5d590a6ad3b97da
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spelling	oai:doaj.org-article:36f501c2bdcd4637b5d590a6ad3b97da2021-11-25T18:38:28ZApplication of TaqMan Real-Time PCR for Detecting ‘<i>Candidatus</i> Arsenophonus Phytopathogenicus’ Infection in Sugar Beet10.3390/pathogens101114662076-0817https://doaj.org/article/36f501c2bdcd4637b5d590a6ad3b97da2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1466https://doaj.org/toc/2076-0817The γ-proteobacterium ‘<i>Candidatus</i> Arsenophonus phytopathogenicus’ is assigned as the major pathogen of “Syndrome des basses richesses”, a sugar beet disease characterised by a reduction in the sugar content of taproots and biomass yield. Despite the economic impact of this bacteriosis, diagnostics for this important pathogen currently rely on end-point PCR detection. Herein, we introduce a TaqMan qPCR for diagnostics of the agent targeting genes encoding a heat shock protein of the Hsp20 family and mannose-6-phosphate isomerase. Quantitation with synthetic oligonucleotides as standard showed that the developed TaqMan qPCR assays enable the detection of up to 100 target copies. A comparison between the TaqMan qPCR and end-point PCR for ‘<i>Ca.</i> A. phytopathogenicus’ detection was carried out on 78 sugar beet samples from different locations in southern Germany. The newly developed assays enable the fast, reliable and sensitive detection of ‘<i>Ca</i>. A. phytopathogenicus’ in sugar beet.Christina ZübertMichael KubeMDPI AGarticleTaqMan qPCR‘<i>Candidatus</i> Arsenophonus phytopathogenicus’SBRsugar beetMedicineRENPathogens, Vol 10, Iss 1466, p 1466 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	TaqMan qPCR ‘<i>Candidatus</i> Arsenophonus phytopathogenicus’ SBR sugar beet Medicine R
spellingShingle	TaqMan qPCR ‘<i>Candidatus</i> Arsenophonus phytopathogenicus’ SBR sugar beet Medicine R Christina Zübert Michael Kube Application of TaqMan Real-Time PCR for Detecting ‘<i>Candidatus</i> Arsenophonus Phytopathogenicus’ Infection in Sugar Beet
description	The γ-proteobacterium ‘<i>Candidatus</i> Arsenophonus phytopathogenicus’ is assigned as the major pathogen of “Syndrome des basses richesses”, a sugar beet disease characterised by a reduction in the sugar content of taproots and biomass yield. Despite the economic impact of this bacteriosis, diagnostics for this important pathogen currently rely on end-point PCR detection. Herein, we introduce a TaqMan qPCR for diagnostics of the agent targeting genes encoding a heat shock protein of the Hsp20 family and mannose-6-phosphate isomerase. Quantitation with synthetic oligonucleotides as standard showed that the developed TaqMan qPCR assays enable the detection of up to 100 target copies. A comparison between the TaqMan qPCR and end-point PCR for ‘<i>Ca.</i> A. phytopathogenicus’ detection was carried out on 78 sugar beet samples from different locations in southern Germany. The newly developed assays enable the fast, reliable and sensitive detection of ‘<i>Ca</i>. A. phytopathogenicus’ in sugar beet.
format	article
author	Christina Zübert Michael Kube
author_facet	Christina Zübert Michael Kube
author_sort	Christina Zübert
title	Application of TaqMan Real-Time PCR for Detecting ‘<i>Candidatus</i> Arsenophonus Phytopathogenicus’ Infection in Sugar Beet
title_short	Application of TaqMan Real-Time PCR for Detecting ‘<i>Candidatus</i> Arsenophonus Phytopathogenicus’ Infection in Sugar Beet
title_full	Application of TaqMan Real-Time PCR for Detecting ‘<i>Candidatus</i> Arsenophonus Phytopathogenicus’ Infection in Sugar Beet
title_fullStr	Application of TaqMan Real-Time PCR for Detecting ‘<i>Candidatus</i> Arsenophonus Phytopathogenicus’ Infection in Sugar Beet
title_full_unstemmed	Application of TaqMan Real-Time PCR for Detecting ‘<i>Candidatus</i> Arsenophonus Phytopathogenicus’ Infection in Sugar Beet
title_sort	application of taqman real-time pcr for detecting ‘<i>candidatus</i> arsenophonus phytopathogenicus’ infection in sugar beet
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/36f501c2bdcd4637b5d590a6ad3b97da
work_keys_str_mv	AT christinazubert applicationoftaqmanrealtimepcrfordetectingicandidatusiarsenophonusphytopathogenicusinfectioninsugarbeet AT michaelkube applicationoftaqmanrealtimepcrfordetectingicandidatusiarsenophonusphytopathogenicusinfectioninsugarbeet
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Application of TaqMan Real-Time PCR for Detecting ‘<i>Candidatus</i> Arsenophonus Phytopathogenicus’ Infection in Sugar Beet

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