Differential contributions of peripheral and central mechanisms to pain in a rodent model of osteoarthritis

Abstract The mechanisms underlying the transition from acute nociceptive pain to centrally maintained chronic pain are not clear. We have studied the contributions of the peripheral and central nervous systems during the development of osteoarthritis (OA) pain. Male Sprague-Dawley rats received unil...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Adrian R. Haywood, Gareth J. Hathway, Victoria Chapman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/36f66a4657fd4e7baa3ccf7573cded97
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:36f66a4657fd4e7baa3ccf7573cded97
record_format	dspace
spelling	oai:doaj.org-article:36f66a4657fd4e7baa3ccf7573cded972021-12-02T11:41:04ZDifferential contributions of peripheral and central mechanisms to pain in a rodent model of osteoarthritis10.1038/s41598-018-25581-82045-2322https://doaj.org/article/36f66a4657fd4e7baa3ccf7573cded972018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25581-8https://doaj.org/toc/2045-2322Abstract The mechanisms underlying the transition from acute nociceptive pain to centrally maintained chronic pain are not clear. We have studied the contributions of the peripheral and central nervous systems during the development of osteoarthritis (OA) pain. Male Sprague-Dawley rats received unilateral intra-articular injections of monosodium iodoacetate (MIA 1 mg) or saline, and weight-bearing (WB) asymmetry and distal allodynia measured. Subgroups of rats received intra-articular injections of, QX-314 (membrane impermeable local anaesthetic) + capsaicin, QX-314, capsaicin or vehicle on days 7, 14 or 28 post-MIA and WB and PWT remeasured. On days 7&14 post-MIA, but not day 28, QX-314 + capsaicin signficantly attenuated changes in WB induced by MIA, illustrating a crucial role for TRPV1 expressing nociceptors in early OA pain. The role of top-down control of spinal excitability was investigated. The mu-opioid receptor agonist DAMGO was microinjected into the rostroventral medulla, to activate endogenous pain modulatory systems, in MIA and control rats and reflex excitability measured using electromyography. DAMGO (3 ng) had a significantly larger inhibitory effect in MIA treated rats than in controls. These data show distinct temporal contribtuions of TRPV1 expressing nociceptors and opioidergic pain control systems at later timepoints.Adrian R. HaywoodGareth J. HathwayVictoria ChapmanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adrian R. Haywood Gareth J. Hathway Victoria Chapman Differential contributions of peripheral and central mechanisms to pain in a rodent model of osteoarthritis
description	Abstract The mechanisms underlying the transition from acute nociceptive pain to centrally maintained chronic pain are not clear. We have studied the contributions of the peripheral and central nervous systems during the development of osteoarthritis (OA) pain. Male Sprague-Dawley rats received unilateral intra-articular injections of monosodium iodoacetate (MIA 1 mg) or saline, and weight-bearing (WB) asymmetry and distal allodynia measured. Subgroups of rats received intra-articular injections of, QX-314 (membrane impermeable local anaesthetic) + capsaicin, QX-314, capsaicin or vehicle on days 7, 14 or 28 post-MIA and WB and PWT remeasured. On days 7&14 post-MIA, but not day 28, QX-314 + capsaicin signficantly attenuated changes in WB induced by MIA, illustrating a crucial role for TRPV1 expressing nociceptors in early OA pain. The role of top-down control of spinal excitability was investigated. The mu-opioid receptor agonist DAMGO was microinjected into the rostroventral medulla, to activate endogenous pain modulatory systems, in MIA and control rats and reflex excitability measured using electromyography. DAMGO (3 ng) had a significantly larger inhibitory effect in MIA treated rats than in controls. These data show distinct temporal contribtuions of TRPV1 expressing nociceptors and opioidergic pain control systems at later timepoints.
format	article
author	Adrian R. Haywood Gareth J. Hathway Victoria Chapman
author_facet	Adrian R. Haywood Gareth J. Hathway Victoria Chapman
author_sort	Adrian R. Haywood
title	Differential contributions of peripheral and central mechanisms to pain in a rodent model of osteoarthritis
title_short	Differential contributions of peripheral and central mechanisms to pain in a rodent model of osteoarthritis
title_full	Differential contributions of peripheral and central mechanisms to pain in a rodent model of osteoarthritis
title_fullStr	Differential contributions of peripheral and central mechanisms to pain in a rodent model of osteoarthritis
title_full_unstemmed	Differential contributions of peripheral and central mechanisms to pain in a rodent model of osteoarthritis
title_sort	differential contributions of peripheral and central mechanisms to pain in a rodent model of osteoarthritis
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/36f66a4657fd4e7baa3ccf7573cded97
work_keys_str_mv	AT adrianrhaywood differentialcontributionsofperipheralandcentralmechanismstopaininarodentmodelofosteoarthritis AT garethjhathway differentialcontributionsofperipheralandcentralmechanismstopaininarodentmodelofosteoarthritis AT victoriachapman differentialcontributionsofperipheralandcentralmechanismstopaininarodentmodelofosteoarthritis
_version_	1718395453766107136

Differential contributions of peripheral and central mechanisms to pain in a rodent model of osteoarthritis

Ejemplares similares