Accelerated proteomic visualization of individual predatory venoms of Conus purpurascens reveals separately evolved predation-evoked venom cabals

Abstract Cone snail venoms have separately evolved for predation and defense. Despite remarkable inter- and intra-species variability, defined sets of synergistic venom peptides (cabals) are considered essential for prey capture by cone snails. To better understand the role of predatory cabals in co...

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Autores principales:	S. W. A. Himaya, Frank Marí, Richard J. Lewis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/36f79e9bb8ce46259b5ec3c88c02c766
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spelling	oai:doaj.org-article:36f79e9bb8ce46259b5ec3c88c02c7662021-12-02T15:08:06ZAccelerated proteomic visualization of individual predatory venoms of Conus purpurascens reveals separately evolved predation-evoked venom cabals10.1038/s41598-017-17422-x2045-2322https://doaj.org/article/36f79e9bb8ce46259b5ec3c88c02c7662018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-17422-xhttps://doaj.org/toc/2045-2322Abstract Cone snail venoms have separately evolved for predation and defense. Despite remarkable inter- and intra-species variability, defined sets of synergistic venom peptides (cabals) are considered essential for prey capture by cone snails. To better understand the role of predatory cabals in cone snails, we used a high-throughput proteomic data mining and visualisation approach. Using this approach, the relationship between the predatory venom peptides from nine C. purpurascens was systematically analysed. Surprisingly, potentially synergistic levels of κ-PVIIA and δ-PVIA were only identified in five of nine specimens. In contrast, the remaining four specimens lacked significant levels of these known excitotoxins and instead contained high levels of the muscle nAChR blockers ψ-PIIIE and αA-PIVA. Interestingly, one of nine specimens expressed both cabals, suggesting that these sub-groups might represent inter-breeding sub-species of C. purpurascens. High throughput cluster analysis also revealed these two cabals clustered with distinct groups of venom peptides that are presently uncharacterised. This is the first report showing that the cone snails of the same species can deploy two separate and distinct predatory cabals for prey capture and shows that the cabals deployed by this species can be more complex than presently realized. Our semi-automated proteomic analysis facilitates the deconvolution of complex venoms to identify co-evolved families of peptides and help unravel their evolutionary relationships in complex venoms.S. W. A. HimayaFrank MaríRichard J. LewisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q S. W. A. Himaya Frank Marí Richard J. Lewis Accelerated proteomic visualization of individual predatory venoms of Conus purpurascens reveals separately evolved predation-evoked venom cabals
description	Abstract Cone snail venoms have separately evolved for predation and defense. Despite remarkable inter- and intra-species variability, defined sets of synergistic venom peptides (cabals) are considered essential for prey capture by cone snails. To better understand the role of predatory cabals in cone snails, we used a high-throughput proteomic data mining and visualisation approach. Using this approach, the relationship between the predatory venom peptides from nine C. purpurascens was systematically analysed. Surprisingly, potentially synergistic levels of κ-PVIIA and δ-PVIA were only identified in five of nine specimens. In contrast, the remaining four specimens lacked significant levels of these known excitotoxins and instead contained high levels of the muscle nAChR blockers ψ-PIIIE and αA-PIVA. Interestingly, one of nine specimens expressed both cabals, suggesting that these sub-groups might represent inter-breeding sub-species of C. purpurascens. High throughput cluster analysis also revealed these two cabals clustered with distinct groups of venom peptides that are presently uncharacterised. This is the first report showing that the cone snails of the same species can deploy two separate and distinct predatory cabals for prey capture and shows that the cabals deployed by this species can be more complex than presently realized. Our semi-automated proteomic analysis facilitates the deconvolution of complex venoms to identify co-evolved families of peptides and help unravel their evolutionary relationships in complex venoms.
format	article
author	S. W. A. Himaya Frank Marí Richard J. Lewis
author_facet	S. W. A. Himaya Frank Marí Richard J. Lewis
author_sort	S. W. A. Himaya
title	Accelerated proteomic visualization of individual predatory venoms of Conus purpurascens reveals separately evolved predation-evoked venom cabals
title_short	Accelerated proteomic visualization of individual predatory venoms of Conus purpurascens reveals separately evolved predation-evoked venom cabals
title_full	Accelerated proteomic visualization of individual predatory venoms of Conus purpurascens reveals separately evolved predation-evoked venom cabals
title_fullStr	Accelerated proteomic visualization of individual predatory venoms of Conus purpurascens reveals separately evolved predation-evoked venom cabals
title_full_unstemmed	Accelerated proteomic visualization of individual predatory venoms of Conus purpurascens reveals separately evolved predation-evoked venom cabals
title_sort	accelerated proteomic visualization of individual predatory venoms of conus purpurascens reveals separately evolved predation-evoked venom cabals
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/36f79e9bb8ce46259b5ec3c88c02c766
work_keys_str_mv	AT swahimaya acceleratedproteomicvisualizationofindividualpredatoryvenomsofconuspurpurascensrevealsseparatelyevolvedpredationevokedvenomcabals AT frankmari acceleratedproteomicvisualizationofindividualpredatoryvenomsofconuspurpurascensrevealsseparatelyevolvedpredationevokedvenomcabals AT richardjlewis acceleratedproteomicvisualizationofindividualpredatoryvenomsofconuspurpurascensrevealsseparatelyevolvedpredationevokedvenomcabals
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Accelerated proteomic visualization of individual predatory venoms of Conus purpurascens reveals separately evolved predation-evoked venom cabals

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