Pemurnian Fikosianin dari Spirulina platensis dengan Metode Liquid Biphasic Flotation (LBF) dan Penentuan Aktivitas Antioksidannya

Phycocyanin from Spirulina platensis (S. Platensis) is a pigment-complex protein belong to the light-harvesting phycobiliprotein family. The pigments have high economic value as a natural blue dye as well as the source of antioxidants and anticancer. Production of pure natural phycocyanin remains in...

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Auteurs principaux: Cori Yolanda Elise, Syafrizayanti Syafrizayanti, Marniati Salim
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Langue:EN
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Publié: Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas 2021
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Accès en ligne:https://doaj.org/article/36f81ca95485431ea8c31a8ca6c62f7d
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spelling oai:doaj.org-article:36f81ca95485431ea8c31a8ca6c62f7d2021-12-02T18:47:31ZPemurnian Fikosianin dari Spirulina platensis dengan Metode Liquid Biphasic Flotation (LBF) dan Penentuan Aktivitas Antioksidannya1978-628X2476-896010.25077/jrk.v12i2.412https://doaj.org/article/36f81ca95485431ea8c31a8ca6c62f7d2021-09-01T00:00:00Zhttp://jrk.fmipa.unand.ac.id/index.php/jrk/article/view/412https://doaj.org/toc/1978-628Xhttps://doaj.org/toc/2476-8960Phycocyanin from Spirulina platensis (S. Platensis) is a pigment-complex protein belong to the light-harvesting phycobiliprotein family. The pigments have high economic value as a natural blue dye as well as the source of antioxidants and anticancer. Production of pure natural phycocyanin remains in high demand. Therefore, this study aimed to obtain phycocyanin with high purity values using modified liquid biphasic flotation (LBF) system and tested for DPPH (1,1-diphenyl-2-pycrilhydrazil) radical scavenging activity. This study produced high purity phycocyanin with purification fold 3.041 ± 0.04 and recovery yields approximately about 70.881%. Purified phycocyanin showed scavenging activity with IC50 of   338.585 mg/mL. Thus, the LBF system yielded high purity phycocyanin pigments.Cori Yolanda EliseSyafrizayanti SyafrizayantiMarniati SalimDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalasarticlephycocyaninspirulina platensisliquid biphasic flotation (lbf)dpphicChemistryQD1-999BiochemistryQD415-436ENIDJurnal Riset Kimia, Vol 12, Iss 2 (2021)
institution DOAJ
collection DOAJ
language EN
ID
topic phycocyanin
spirulina platensis
liquid biphasic flotation (lbf)
dpph
ic
Chemistry
QD1-999
Biochemistry
QD415-436
spellingShingle phycocyanin
spirulina platensis
liquid biphasic flotation (lbf)
dpph
ic
Chemistry
QD1-999
Biochemistry
QD415-436
Cori Yolanda Elise
Syafrizayanti Syafrizayanti
Marniati Salim
Pemurnian Fikosianin dari Spirulina platensis dengan Metode Liquid Biphasic Flotation (LBF) dan Penentuan Aktivitas Antioksidannya
description Phycocyanin from Spirulina platensis (S. Platensis) is a pigment-complex protein belong to the light-harvesting phycobiliprotein family. The pigments have high economic value as a natural blue dye as well as the source of antioxidants and anticancer. Production of pure natural phycocyanin remains in high demand. Therefore, this study aimed to obtain phycocyanin with high purity values using modified liquid biphasic flotation (LBF) system and tested for DPPH (1,1-diphenyl-2-pycrilhydrazil) radical scavenging activity. This study produced high purity phycocyanin with purification fold 3.041 ± 0.04 and recovery yields approximately about 70.881%. Purified phycocyanin showed scavenging activity with IC50 of   338.585 mg/mL. Thus, the LBF system yielded high purity phycocyanin pigments.
format article
author Cori Yolanda Elise
Syafrizayanti Syafrizayanti
Marniati Salim
author_facet Cori Yolanda Elise
Syafrizayanti Syafrizayanti
Marniati Salim
author_sort Cori Yolanda Elise
title Pemurnian Fikosianin dari Spirulina platensis dengan Metode Liquid Biphasic Flotation (LBF) dan Penentuan Aktivitas Antioksidannya
title_short Pemurnian Fikosianin dari Spirulina platensis dengan Metode Liquid Biphasic Flotation (LBF) dan Penentuan Aktivitas Antioksidannya
title_full Pemurnian Fikosianin dari Spirulina platensis dengan Metode Liquid Biphasic Flotation (LBF) dan Penentuan Aktivitas Antioksidannya
title_fullStr Pemurnian Fikosianin dari Spirulina platensis dengan Metode Liquid Biphasic Flotation (LBF) dan Penentuan Aktivitas Antioksidannya
title_full_unstemmed Pemurnian Fikosianin dari Spirulina platensis dengan Metode Liquid Biphasic Flotation (LBF) dan Penentuan Aktivitas Antioksidannya
title_sort pemurnian fikosianin dari spirulina platensis dengan metode liquid biphasic flotation (lbf) dan penentuan aktivitas antioksidannya
publisher Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas
publishDate 2021
url https://doaj.org/article/36f81ca95485431ea8c31a8ca6c62f7d
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