Evaluation method for asymmetric uncertainty of quantitative polymerase chain reaction measurements of deoxyribonucleic acids with low copy number

Abstract Recently, in food safety and various other fields, qualitative and quantitative gene analysis using real-time polymerase chain reaction (PCR) method has become increasingly popular. The limit of detection (LOD) and quantifiable range for these measurements depends on the range and precision...

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Autores principales: Unoh Ki, Takeru Suzuki, Satoshi Nakazawa, Yuuki Yonekawa, Kazuki Watanabe, Michie Hashimoto, Shigeo Hatada, Hirotaka Unno
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/37017f5d07c74d3abb547ec4bdde3f85
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