Network Pharmacology and Experimental Assessment to Explore the Pharmacological Mechanism of Qimai Feiluoping Decoction Against Pulmonary Fibrosis
Pulmonary fibrosis (PF) is one of the pathologic changes in COVID-19 patients in convalescence, and it is also a potential long-term sequela in severe COVID-19 patients. Qimai Feiluoping decoction (QM) is a traditional Chinese medicine formula recommended in the Chinese national medical program for...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Frontiers Media S.A.
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/37038ecd3f8e48de9b064789cccb8ed2 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:37038ecd3f8e48de9b064789cccb8ed2 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:37038ecd3f8e48de9b064789cccb8ed22021-12-03T06:46:26ZNetwork Pharmacology and Experimental Assessment to Explore the Pharmacological Mechanism of Qimai Feiluoping Decoction Against Pulmonary Fibrosis1663-981210.3389/fphar.2021.770197https://doaj.org/article/37038ecd3f8e48de9b064789cccb8ed22021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.770197/fullhttps://doaj.org/toc/1663-9812Pulmonary fibrosis (PF) is one of the pathologic changes in COVID-19 patients in convalescence, and it is also a potential long-term sequela in severe COVID-19 patients. Qimai Feiluoping decoction (QM) is a traditional Chinese medicine formula recommended in the Chinese national medical program for COVID-19 convalescent patients, and PF is one of its indications. Through clinical observation, QM was found to improve the clinical symptoms and pulmonary function and reduce the degree of PF of COVID-19 convalescent patients. To further explore the pharmacological mechanisms and possible active components of QM in anti-PF effect, UHPLC/Q-TOF-MS was used to analyze the composition of the QM extract and the active components that can be absorbed into the blood, leading to the identification of 56 chemical compounds and 10 active components. Then, network pharmacology was used to predict the potential mechanisms and targets of QM; it predicted that QM exerts its anti-PF effects via the regulation of the epithelial–mesenchymal transition (EMT), extracellular matrix (ECM) degradation, and TGF-β signaling pathway. Finally, TGF-β1–induced A549 cells were used to verify and explore the pharmacological effects of QM and found that QM could inhibit the proliferation of TGF-β1–induced A549 cells, attenuate EMT, and promote ECM degradation by inhibiting the TGF-β/Smad3 pathway.Yingying YangYingying YangLu DingTingting BaoTingting BaoYaxin LiJing MaQingwei LiZezheng GaoSiyu SongJing WangJiachao ZhaoZiyuan WangDaqing ZhaoXiangyan LiZeyu WangLinhua ZhaoXiaolin TongFrontiers Media S.A.articleCOVID-19pulmonary fibrosisQM formulanetwork pharmacologyepithelial–mesenchymal transitionextracellular matrix accumulationTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
COVID-19 pulmonary fibrosis QM formula network pharmacology epithelial–mesenchymal transition extracellular matrix accumulation Therapeutics. Pharmacology RM1-950 |
| spellingShingle |
COVID-19 pulmonary fibrosis QM formula network pharmacology epithelial–mesenchymal transition extracellular matrix accumulation Therapeutics. Pharmacology RM1-950 Yingying Yang Yingying Yang Lu Ding Tingting Bao Tingting Bao Yaxin Li Jing Ma Qingwei Li Zezheng Gao Siyu Song Jing Wang Jiachao Zhao Ziyuan Wang Daqing Zhao Xiangyan Li Zeyu Wang Linhua Zhao Xiaolin Tong Network Pharmacology and Experimental Assessment to Explore the Pharmacological Mechanism of Qimai Feiluoping Decoction Against Pulmonary Fibrosis |
| description |
Pulmonary fibrosis (PF) is one of the pathologic changes in COVID-19 patients in convalescence, and it is also a potential long-term sequela in severe COVID-19 patients. Qimai Feiluoping decoction (QM) is a traditional Chinese medicine formula recommended in the Chinese national medical program for COVID-19 convalescent patients, and PF is one of its indications. Through clinical observation, QM was found to improve the clinical symptoms and pulmonary function and reduce the degree of PF of COVID-19 convalescent patients. To further explore the pharmacological mechanisms and possible active components of QM in anti-PF effect, UHPLC/Q-TOF-MS was used to analyze the composition of the QM extract and the active components that can be absorbed into the blood, leading to the identification of 56 chemical compounds and 10 active components. Then, network pharmacology was used to predict the potential mechanisms and targets of QM; it predicted that QM exerts its anti-PF effects via the regulation of the epithelial–mesenchymal transition (EMT), extracellular matrix (ECM) degradation, and TGF-β signaling pathway. Finally, TGF-β1–induced A549 cells were used to verify and explore the pharmacological effects of QM and found that QM could inhibit the proliferation of TGF-β1–induced A549 cells, attenuate EMT, and promote ECM degradation by inhibiting the TGF-β/Smad3 pathway. |
| format |
article |
| author |
Yingying Yang Yingying Yang Lu Ding Tingting Bao Tingting Bao Yaxin Li Jing Ma Qingwei Li Zezheng Gao Siyu Song Jing Wang Jiachao Zhao Ziyuan Wang Daqing Zhao Xiangyan Li Zeyu Wang Linhua Zhao Xiaolin Tong |
| author_facet |
Yingying Yang Yingying Yang Lu Ding Tingting Bao Tingting Bao Yaxin Li Jing Ma Qingwei Li Zezheng Gao Siyu Song Jing Wang Jiachao Zhao Ziyuan Wang Daqing Zhao Xiangyan Li Zeyu Wang Linhua Zhao Xiaolin Tong |
| author_sort |
Yingying Yang |
| title |
Network Pharmacology and Experimental Assessment to Explore the Pharmacological Mechanism of Qimai Feiluoping Decoction Against Pulmonary Fibrosis |
| title_short |
Network Pharmacology and Experimental Assessment to Explore the Pharmacological Mechanism of Qimai Feiluoping Decoction Against Pulmonary Fibrosis |
| title_full |
Network Pharmacology and Experimental Assessment to Explore the Pharmacological Mechanism of Qimai Feiluoping Decoction Against Pulmonary Fibrosis |
| title_fullStr |
Network Pharmacology and Experimental Assessment to Explore the Pharmacological Mechanism of Qimai Feiluoping Decoction Against Pulmonary Fibrosis |
| title_full_unstemmed |
Network Pharmacology and Experimental Assessment to Explore the Pharmacological Mechanism of Qimai Feiluoping Decoction Against Pulmonary Fibrosis |
| title_sort |
network pharmacology and experimental assessment to explore the pharmacological mechanism of qimai feiluoping decoction against pulmonary fibrosis |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/37038ecd3f8e48de9b064789cccb8ed2 |
| work_keys_str_mv |
AT yingyingyang networkpharmacologyandexperimentalassessmenttoexplorethepharmacologicalmechanismofqimaifeiluopingdecoctionagainstpulmonaryfibrosis AT yingyingyang networkpharmacologyandexperimentalassessmenttoexplorethepharmacologicalmechanismofqimaifeiluopingdecoctionagainstpulmonaryfibrosis AT luding networkpharmacologyandexperimentalassessmenttoexplorethepharmacologicalmechanismofqimaifeiluopingdecoctionagainstpulmonaryfibrosis AT tingtingbao networkpharmacologyandexperimentalassessmenttoexplorethepharmacologicalmechanismofqimaifeiluopingdecoctionagainstpulmonaryfibrosis AT tingtingbao networkpharmacologyandexperimentalassessmenttoexplorethepharmacologicalmechanismofqimaifeiluopingdecoctionagainstpulmonaryfibrosis AT yaxinli networkpharmacologyandexperimentalassessmenttoexplorethepharmacologicalmechanismofqimaifeiluopingdecoctionagainstpulmonaryfibrosis AT jingma networkpharmacologyandexperimentalassessmenttoexplorethepharmacologicalmechanismofqimaifeiluopingdecoctionagainstpulmonaryfibrosis AT qingweili networkpharmacologyandexperimentalassessmenttoexplorethepharmacologicalmechanismofqimaifeiluopingdecoctionagainstpulmonaryfibrosis AT zezhenggao networkpharmacologyandexperimentalassessmenttoexplorethepharmacologicalmechanismofqimaifeiluopingdecoctionagainstpulmonaryfibrosis AT siyusong networkpharmacologyandexperimentalassessmenttoexplorethepharmacologicalmechanismofqimaifeiluopingdecoctionagainstpulmonaryfibrosis AT jingwang networkpharmacologyandexperimentalassessmenttoexplorethepharmacologicalmechanismofqimaifeiluopingdecoctionagainstpulmonaryfibrosis AT jiachaozhao networkpharmacologyandexperimentalassessmenttoexplorethepharmacologicalmechanismofqimaifeiluopingdecoctionagainstpulmonaryfibrosis AT ziyuanwang networkpharmacologyandexperimentalassessmenttoexplorethepharmacologicalmechanismofqimaifeiluopingdecoctionagainstpulmonaryfibrosis AT daqingzhao networkpharmacologyandexperimentalassessmenttoexplorethepharmacologicalmechanismofqimaifeiluopingdecoctionagainstpulmonaryfibrosis AT xiangyanli networkpharmacologyandexperimentalassessmenttoexplorethepharmacologicalmechanismofqimaifeiluopingdecoctionagainstpulmonaryfibrosis AT zeyuwang networkpharmacologyandexperimentalassessmenttoexplorethepharmacologicalmechanismofqimaifeiluopingdecoctionagainstpulmonaryfibrosis AT linhuazhao networkpharmacologyandexperimentalassessmenttoexplorethepharmacologicalmechanismofqimaifeiluopingdecoctionagainstpulmonaryfibrosis AT xiaolintong networkpharmacologyandexperimentalassessmenttoexplorethepharmacologicalmechanismofqimaifeiluopingdecoctionagainstpulmonaryfibrosis |
| _version_ |
1718373863917617152 |