CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation
CRISPR-Cas9 genome editing is presumed to knock out gene function by generating a frameshift during NHEJ repair. Here, the authors investigate mRNA and protein expression in edited lines and find genome editing can generate internal ribosome entry sites or alternatively spliced variants.
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Nature Portfolio
2019
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oai:doaj.org-article:370492b1e1f04ee38f9a459c9cce9e772021-12-02T17:01:50ZCRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation10.1038/s41467-019-12028-52041-1723https://doaj.org/article/370492b1e1f04ee38f9a459c9cce9e772019-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12028-5https://doaj.org/toc/2041-1723CRISPR-Cas9 genome editing is presumed to knock out gene function by generating a frameshift during NHEJ repair. Here, the authors investigate mRNA and protein expression in edited lines and find genome editing can generate internal ribosome entry sites or alternatively spliced variants.Rubina TuladharYunku YeuJohn Tyler PiazzaZhen TanJean Rene ClemenceauXiaofeng WuQuinn BarrettJeremiah HerbertDavid H. MathewsJames KimTae Hyun HwangLawrence LumNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Science Q |
| spellingShingle |
Science Q Rubina Tuladhar Yunku Yeu John Tyler Piazza Zhen Tan Jean Rene Clemenceau Xiaofeng Wu Quinn Barrett Jeremiah Herbert David H. Mathews James Kim Tae Hyun Hwang Lawrence Lum CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation |
| description |
CRISPR-Cas9 genome editing is presumed to knock out gene function by generating a frameshift during NHEJ repair. Here, the authors investigate mRNA and protein expression in edited lines and find genome editing can generate internal ribosome entry sites or alternatively spliced variants. |
| format |
article |
| author |
Rubina Tuladhar Yunku Yeu John Tyler Piazza Zhen Tan Jean Rene Clemenceau Xiaofeng Wu Quinn Barrett Jeremiah Herbert David H. Mathews James Kim Tae Hyun Hwang Lawrence Lum |
| author_facet |
Rubina Tuladhar Yunku Yeu John Tyler Piazza Zhen Tan Jean Rene Clemenceau Xiaofeng Wu Quinn Barrett Jeremiah Herbert David H. Mathews James Kim Tae Hyun Hwang Lawrence Lum |
| author_sort |
Rubina Tuladhar |
| title |
CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation |
| title_short |
CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation |
| title_full |
CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation |
| title_fullStr |
CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation |
| title_full_unstemmed |
CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation |
| title_sort |
crispr-cas9-based mutagenesis frequently provokes on-target mrna misregulation |
| publisher |
Nature Portfolio |
| publishDate |
2019 |
| url |
https://doaj.org/article/370492b1e1f04ee38f9a459c9cce9e77 |
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