CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation

CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation

CRISPR-Cas9 genome editing is presumed to knock out gene function by generating a frameshift during NHEJ repair. Here, the authors investigate mRNA and protein expression in edited lines and find genome editing can generate internal ribosome entry sites or alternatively spliced variants.

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Autores principales: Rubina Tuladhar, Yunku Yeu, John Tyler Piazza, Zhen Tan, Jean Rene Clemenceau, Xiaofeng Wu, Quinn Barrett, Jeremiah Herbert, David H. Mathews, James Kim, Tae Hyun Hwang, Lawrence Lum
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
Materias:
Science
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Acceso en línea:https://doaj.org/article/370492b1e1f04ee38f9a459c9cce9e77
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