Mycobacterium tuberculosis modifies cell wall carbohydrates during biofilm growth with a concomitant reduction in complement activation

The development of new vaccines for TB needs to be underpinned by an understanding of both the molecular and cellular mechanisms of host-pathogen interactions and how the immune response can be modulated to achieve protection from disease. Complement orchestrates many aspects of the innate and adapt...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Thomas Keating, Samuel Lethbridge, Jon C. Allnutt, Charlotte L. Hendon-Dunn, Stephen R. Thomas, Luke J. Alderwick, Stephen C. Taylor, Joanna Bacon
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Mycobacterium tuberculosis biofilm complement deposition alpha glucan MBL C1q Cytology QH573-671
Acceso en línea:	https://doaj.org/article/37062073aa574ee2953b77b48421864b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:37062073aa574ee2953b77b48421864b
record_format	dspace
spelling	oai:doaj.org-article:37062073aa574ee2953b77b48421864b2021-11-06T04:33:09ZMycobacterium tuberculosis modifies cell wall carbohydrates during biofilm growth with a concomitant reduction in complement activation2468-233010.1016/j.tcsw.2021.100065https://doaj.org/article/37062073aa574ee2953b77b48421864b2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2468233021000189https://doaj.org/toc/2468-2330The development of new vaccines for TB needs to be underpinned by an understanding of both the molecular and cellular mechanisms of host-pathogen interactions and how the immune response can be modulated to achieve protection from disease. Complement orchestrates many aspects of the innate and adaptive immune responses. However, little is known about the contribution of the complement pathways during TB disease, particularly with respect to mycobacterial phenotype. Extracellular communities (biofilms) of M. tuberculosis are found in the acellular rim of granulomas, during disease, and these are likely to be present in post-primary TB episodes, in necrotic lesions. Our study aimed to determine which mycobacterial cell wall components were altered during biofilm growth and how these cell wall alterations modified the complement response. We have shown that M. tuberculosis biofilms modified their cell wall carbohydrates and elicited reduced classical and lectin pathway activation. Consistent with this finding was the reduction of C3b/iC3b deposition on biofilm cell wall carbohydrate extracts. Here, we have highlighted the role of cell wall carbohydrate alterations during biofilm growth of M. tuberculosis and subsequent modulation of complement activation.Thomas KeatingSamuel LethbridgeJon C. AllnuttCharlotte L. Hendon-DunnStephen R. ThomasLuke J. AlderwickStephen C. TaylorJoanna BaconElsevierarticleMycobacterium tuberculosisbiofilmcomplement depositionalpha glucanMBLC1qCytologyQH573-671ENThe Cell Surface, Vol 7, Iss , Pp 100065- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mycobacterium tuberculosis biofilm complement deposition alpha glucan MBL C1q Cytology QH573-671
spellingShingle	Mycobacterium tuberculosis biofilm complement deposition alpha glucan MBL C1q Cytology QH573-671 Thomas Keating Samuel Lethbridge Jon C. Allnutt Charlotte L. Hendon-Dunn Stephen R. Thomas Luke J. Alderwick Stephen C. Taylor Joanna Bacon Mycobacterium tuberculosis modifies cell wall carbohydrates during biofilm growth with a concomitant reduction in complement activation
description	The development of new vaccines for TB needs to be underpinned by an understanding of both the molecular and cellular mechanisms of host-pathogen interactions and how the immune response can be modulated to achieve protection from disease. Complement orchestrates many aspects of the innate and adaptive immune responses. However, little is known about the contribution of the complement pathways during TB disease, particularly with respect to mycobacterial phenotype. Extracellular communities (biofilms) of M. tuberculosis are found in the acellular rim of granulomas, during disease, and these are likely to be present in post-primary TB episodes, in necrotic lesions. Our study aimed to determine which mycobacterial cell wall components were altered during biofilm growth and how these cell wall alterations modified the complement response. We have shown that M. tuberculosis biofilms modified their cell wall carbohydrates and elicited reduced classical and lectin pathway activation. Consistent with this finding was the reduction of C3b/iC3b deposition on biofilm cell wall carbohydrate extracts. Here, we have highlighted the role of cell wall carbohydrate alterations during biofilm growth of M. tuberculosis and subsequent modulation of complement activation.
format	article
author	Thomas Keating Samuel Lethbridge Jon C. Allnutt Charlotte L. Hendon-Dunn Stephen R. Thomas Luke J. Alderwick Stephen C. Taylor Joanna Bacon
author_facet	Thomas Keating Samuel Lethbridge Jon C. Allnutt Charlotte L. Hendon-Dunn Stephen R. Thomas Luke J. Alderwick Stephen C. Taylor Joanna Bacon
author_sort	Thomas Keating
title	Mycobacterium tuberculosis modifies cell wall carbohydrates during biofilm growth with a concomitant reduction in complement activation
title_short	Mycobacterium tuberculosis modifies cell wall carbohydrates during biofilm growth with a concomitant reduction in complement activation
title_full	Mycobacterium tuberculosis modifies cell wall carbohydrates during biofilm growth with a concomitant reduction in complement activation
title_fullStr	Mycobacterium tuberculosis modifies cell wall carbohydrates during biofilm growth with a concomitant reduction in complement activation
title_full_unstemmed	Mycobacterium tuberculosis modifies cell wall carbohydrates during biofilm growth with a concomitant reduction in complement activation
title_sort	mycobacterium tuberculosis modifies cell wall carbohydrates during biofilm growth with a concomitant reduction in complement activation
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/37062073aa574ee2953b77b48421864b
work_keys_str_mv	AT thomaskeating mycobacteriumtuberculosismodifiescellwallcarbohydratesduringbiofilmgrowthwithaconcomitantreductionincomplementactivation AT samuellethbridge mycobacteriumtuberculosismodifiescellwallcarbohydratesduringbiofilmgrowthwithaconcomitantreductionincomplementactivation AT joncallnutt mycobacteriumtuberculosismodifiescellwallcarbohydratesduringbiofilmgrowthwithaconcomitantreductionincomplementactivation AT charlottelhendondunn mycobacteriumtuberculosismodifiescellwallcarbohydratesduringbiofilmgrowthwithaconcomitantreductionincomplementactivation AT stephenrthomas mycobacteriumtuberculosismodifiescellwallcarbohydratesduringbiofilmgrowthwithaconcomitantreductionincomplementactivation AT lukejalderwick mycobacteriumtuberculosismodifiescellwallcarbohydratesduringbiofilmgrowthwithaconcomitantreductionincomplementactivation AT stephenctaylor mycobacteriumtuberculosismodifiescellwallcarbohydratesduringbiofilmgrowthwithaconcomitantreductionincomplementactivation AT joannabacon mycobacteriumtuberculosismodifiescellwallcarbohydratesduringbiofilmgrowthwithaconcomitantreductionincomplementactivation
_version_	1718443846658949120

Mycobacterium tuberculosis modifies cell wall carbohydrates during biofilm growth with a concomitant reduction in complement activation

Ejemplares similares