Two TGA Transcription Factor Members from Hyper-Susceptible Soybean Exhibiting Significant Basal Resistance to Soybean mosaic virus

TGA transcription factors (TFs) exhibit basal resistance in Arabidopsis, but susceptibility to a pathogen attack in tomatoes; however, their roles in soybean (Glycine max) to Soybean mosaic virus (SMV) are unknown. In this study, 27 T...

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Autores principales:	Hua Jiang, Shengyu Gu, Kai Li, Junyi Gai
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	soybean (<i>Glycine max</i> (L.) Merr.) TGA transcription factor molecular characterization <i>Soybean mosaic virus</i> <i>Nicotiana benthamiana</i> basal resistance Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/3707c4e6b6114afd81766219196bab20
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spelling	oai:doaj.org-article:3707c4e6b6114afd81766219196bab202021-11-11T16:48:41ZTwo TGA Transcription Factor Members from Hyper-Susceptible Soybean Exhibiting Significant Basal Resistance to <i>Soybean mosaic virus</i>10.3390/ijms2221113291422-00671661-6596https://doaj.org/article/3707c4e6b6114afd81766219196bab202021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11329https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067TGA transcription factors (TFs) exhibit basal resistance in <i>Arabidopsis</i>, but susceptibility to a pathogen attack in tomatoes; however, their roles in soybean (<i>Glycine max</i>) to <i>Soybean mosaic virus</i> (SMV) are unknown. In this study, 27 <i>TGA</i> genes were isolated from a SMV hyper-susceptible soybean <i>NN1138-2</i>, designated <i>GmTGA1</i>~<i>GmTGA27</i>, which were clustered into seven phylogenetic groups. The expression profiles of <i>GmTGAs</i> showed that the highly expressed genes were mainly in Groups I, II, and VII under non-induction conditions, while out of the 27 <i>GmTGAs</i>, 19 responded to SMV-induction. Interestingly, in further transient <i>N. benthamiana</i>-SMV pathosystem assay, all the 19 <i>GmTGAs</i> overexpressed did not promote SMV infection in inoculated leaves, but they exhibited basal resistance except one without function. Among the 18 functional ones, GmTGA8 and GmTGA19, with similar motif distribution, nuclear localization sequence and interaction proteins, showed a rapid response to SMV infection and performed better than the others in inhibiting SMV multiplication. This finding suggested that GmTGA TFs may support basal resistance to SMV even from a hyper-susceptible source. What the mechanism of the genes (<i>GmTGA8</i>, <i>GmTGA19</i>, etc.) with basal resistance to SMV is and what their potential for the future improvement of resistance to SMV in soybeans is, are to be explored.Hua JiangShengyu GuKai LiJunyi GaiMDPI AGarticlesoybean (<i>Glycine max</i> (L.) Merr.)TGA transcription factormolecular characterization<i>Soybean mosaic virus</i><i>Nicotiana benthamiana</i>basal resistanceBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11329, p 11329 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	soybean (<i>Glycine max</i> (L.) Merr.) TGA transcription factor molecular characterization <i>Soybean mosaic virus</i> <i>Nicotiana benthamiana</i> basal resistance Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	soybean (<i>Glycine max</i> (L.) Merr.) TGA transcription factor molecular characterization <i>Soybean mosaic virus</i> <i>Nicotiana benthamiana</i> basal resistance Biology (General) QH301-705.5 Chemistry QD1-999 Hua Jiang Shengyu Gu Kai Li Junyi Gai Two TGA Transcription Factor Members from Hyper-Susceptible Soybean Exhibiting Significant Basal Resistance to <i>Soybean mosaic virus</i>
description	TGA transcription factors (TFs) exhibit basal resistance in <i>Arabidopsis</i>, but susceptibility to a pathogen attack in tomatoes; however, their roles in soybean (<i>Glycine max</i>) to <i>Soybean mosaic virus</i> (SMV) are unknown. In this study, 27 <i>TGA</i> genes were isolated from a SMV hyper-susceptible soybean <i>NN1138-2</i>, designated <i>GmTGA1</i>~<i>GmTGA27</i>, which were clustered into seven phylogenetic groups. The expression profiles of <i>GmTGAs</i> showed that the highly expressed genes were mainly in Groups I, II, and VII under non-induction conditions, while out of the 27 <i>GmTGAs</i>, 19 responded to SMV-induction. Interestingly, in further transient <i>N. benthamiana</i>-SMV pathosystem assay, all the 19 <i>GmTGAs</i> overexpressed did not promote SMV infection in inoculated leaves, but they exhibited basal resistance except one without function. Among the 18 functional ones, GmTGA8 and GmTGA19, with similar motif distribution, nuclear localization sequence and interaction proteins, showed a rapid response to SMV infection and performed better than the others in inhibiting SMV multiplication. This finding suggested that GmTGA TFs may support basal resistance to SMV even from a hyper-susceptible source. What the mechanism of the genes (<i>GmTGA8</i>, <i>GmTGA19</i>, etc.) with basal resistance to SMV is and what their potential for the future improvement of resistance to SMV in soybeans is, are to be explored.
format	article
author	Hua Jiang Shengyu Gu Kai Li Junyi Gai
author_facet	Hua Jiang Shengyu Gu Kai Li Junyi Gai
author_sort	Hua Jiang
title	Two TGA Transcription Factor Members from Hyper-Susceptible Soybean Exhibiting Significant Basal Resistance to <i>Soybean mosaic virus</i>
title_short	Two TGA Transcription Factor Members from Hyper-Susceptible Soybean Exhibiting Significant Basal Resistance to <i>Soybean mosaic virus</i>
title_full	Two TGA Transcription Factor Members from Hyper-Susceptible Soybean Exhibiting Significant Basal Resistance to <i>Soybean mosaic virus</i>
title_fullStr	Two TGA Transcription Factor Members from Hyper-Susceptible Soybean Exhibiting Significant Basal Resistance to <i>Soybean mosaic virus</i>
title_full_unstemmed	Two TGA Transcription Factor Members from Hyper-Susceptible Soybean Exhibiting Significant Basal Resistance to <i>Soybean mosaic virus</i>
title_sort	two tga transcription factor members from hyper-susceptible soybean exhibiting significant basal resistance to <i>soybean mosaic virus</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3707c4e6b6114afd81766219196bab20
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Two TGA Transcription Factor Members from Hyper-Susceptible Soybean Exhibiting Significant Basal Resistance to <i>Soybean mosaic virus</i>

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