Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration.

The use of copper (Cu2+) and cobalt (Co2+) has been described to stimulate blood vessel formation, a key process for the success of tissue regeneration. However, understanding how different concentrations of these ions affect cellular response is important to design scaffolds for their delivery to b...

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Autores principales:	Elia Bosch-Rué, Leire Díez-Tercero, Raquel Rodríguez-González, Begoña María Bosch-Canals, Roman A Perez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/370907ca51064e20a6f4bc8bdf7b4a18
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spelling	oai:doaj.org-article:370907ca51064e20a6f4bc8bdf7b4a182021-12-02T20:13:25ZAssessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration.1932-620310.1371/journal.pone.0259125https://doaj.org/article/370907ca51064e20a6f4bc8bdf7b4a182021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259125https://doaj.org/toc/1932-6203The use of copper (Cu2+) and cobalt (Co2+) has been described to stimulate blood vessel formation, a key process for the success of tissue regeneration. However, understanding how different concentrations of these ions affect cellular response is important to design scaffolds for their delivery to better fine tune the angiogenic response. On the one hand, gene expression analysis and the assessment of tubular formation structures with human umbilical vein endothelial cells (HUVEC) revealed that high concentrations (10μM) of Cu2+ in early times and lower concentrations (0.1 and 1μM) at later times (day 7) enhanced angiogenic response. On the other hand, higher concentrations (25μM) of Co2+ during all time course increased the angiogenic gene expression and 0.5, 5 and 25μM enhanced the ability to form tubular structures. To further explore synergistic effects combining both ions, the non-toxic concentrations were used simultaneously, although results showed an increased cell toxicity and no improvement of angiogenic response. These results provide useful information for the design of Cu2+ or Co2+ delivery scaffolds in order to release the appropriate concentration during time course for blood vessel stimulation.Elia Bosch-RuéLeire Díez-TerceroRaquel Rodríguez-GonzálezBegoña María Bosch-CanalsRoman A PerezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0259125 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elia Bosch-Rué Leire Díez-Tercero Raquel Rodríguez-González Begoña María Bosch-Canals Roman A Perez Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration.
description	The use of copper (Cu2+) and cobalt (Co2+) has been described to stimulate blood vessel formation, a key process for the success of tissue regeneration. However, understanding how different concentrations of these ions affect cellular response is important to design scaffolds for their delivery to better fine tune the angiogenic response. On the one hand, gene expression analysis and the assessment of tubular formation structures with human umbilical vein endothelial cells (HUVEC) revealed that high concentrations (10μM) of Cu2+ in early times and lower concentrations (0.1 and 1μM) at later times (day 7) enhanced angiogenic response. On the other hand, higher concentrations (25μM) of Co2+ during all time course increased the angiogenic gene expression and 0.5, 5 and 25μM enhanced the ability to form tubular structures. To further explore synergistic effects combining both ions, the non-toxic concentrations were used simultaneously, although results showed an increased cell toxicity and no improvement of angiogenic response. These results provide useful information for the design of Cu2+ or Co2+ delivery scaffolds in order to release the appropriate concentration during time course for blood vessel stimulation.
format	article
author	Elia Bosch-Rué Leire Díez-Tercero Raquel Rodríguez-González Begoña María Bosch-Canals Roman A Perez
author_facet	Elia Bosch-Rué Leire Díez-Tercero Raquel Rodríguez-González Begoña María Bosch-Canals Roman A Perez
author_sort	Elia Bosch-Rué
title	Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration.
title_short	Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration.
title_full	Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration.
title_fullStr	Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration.
title_full_unstemmed	Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration.
title_sort	assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/370907ca51064e20a6f4bc8bdf7b4a18
work_keys_str_mv	AT eliaboschrue assessingthepotentialroleofcopperandcobaltinstimulatingangiogenesisfortissueregeneration AT leiredieztercero assessingthepotentialroleofcopperandcobaltinstimulatingangiogenesisfortissueregeneration AT raquelrodriguezgonzalez assessingthepotentialroleofcopperandcobaltinstimulatingangiogenesisfortissueregeneration AT begonamariaboschcanals assessingthepotentialroleofcopperandcobaltinstimulatingangiogenesisfortissueregeneration AT romanaperez assessingthepotentialroleofcopperandcobaltinstimulatingangiogenesisfortissueregeneration
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Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration.

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