Antioxidant and hepatoprotective role of gold nanoparticles against murine hepatic schistosomiasis

Mohamed A Dkhil,1,2 Amira A Bauomy,2,3 Marwa SM Diab,4 Saleh Al-Quraishy21Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt; 3Department of Laboratory Sciences, College of...

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Autores principales:	Dkhil MA, Bauomy AA, Diab MSM, Al-Quraishy S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Nanogold Schistosoma mansoni liver gene expressions histopathology oxidative stress mice. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/370bd3493e0c42be9c5e1d40cdd7cfcf
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spelling	oai:doaj.org-article:370bd3493e0c42be9c5e1d40cdd7cfcf2021-12-02T03:05:17ZAntioxidant and hepatoprotective role of gold nanoparticles against murine hepatic schistosomiasis1178-2013https://doaj.org/article/370bd3493e0c42be9c5e1d40cdd7cfcf2015-12-01T00:00:00Zhttps://www.dovepress.com/antioxidant-and-hepatoprotective-role-of-gold-nanoparticles-against-mu-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Mohamed A Dkhil,1,2 Amira A Bauomy,2,3 Marwa SM Diab,4 Saleh Al-Quraishy21Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt; 3Department of Laboratory Sciences, College of Science & Arts, Qassim University, Buraydah, Saudi Arabia; 4Molecular Drug Evaluation Department, National Organization for Drug Control & Research (NODCAR), Giza, EgyptAbstract: In recent years, gold nanoparticles (AuNPs) have become the focus of much attention in biomedical research, especially in the context of nanomedicine, due to their distinctive physicochemical properties. The current study was planned to assess the effect of three dose levels of AuNPs on the gene expression, histology, and oxidative stress status of Schistosoma mansoni-infected mice liver. Inoculation of mice with 100 µL AuNPs at different doses (0.25, 0.5, and 1 mg/kg mice body weight) twice on day 46 and day 49 postinfection reduced the total worm burden, the egg load in the liver, and the granuloma size. AuNPs also appeared to decrease the activities of malondialdehyde and nitric oxide significantly, and increase the level of glutathione compared to the infected untreated group. Concomitantly, AuNPs ameliorated the inflammatory response by decreasing the mRNA expression of interleukin-1β, interleukin-6, tumor necrosis factor-α, interferon-γ, and inducible nitric oxide synthase. These consistent molecular, histopathological, and biochemical data suggest that AuNPs could ameliorate infection-induced damage in the livers of mice. Our results indicated that AuNPs are effective anti-schistosomal and antioxidant agents. Further confirmation of the role of nanogold as an anti-schistosomal agent, as well as its mechanism of action, requires further studies to be undertaken in the future.Keywords: nanogold, Schistosoma mansoni, liver, gene expressions, histopathology, oxidative stress, miceDkhil MABauomy AADiab MSMAl-Quraishy SDove Medical PressarticleNanogoldSchistosoma mansonilivergene expressionshistopathologyoxidative stressmice.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 7467-7475 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nanogold Schistosoma mansoni liver gene expressions histopathology oxidative stress mice. Medicine (General) R5-920
spellingShingle	Nanogold Schistosoma mansoni liver gene expressions histopathology oxidative stress mice. Medicine (General) R5-920 Dkhil MA Bauomy AA Diab MSM Al-Quraishy S Antioxidant and hepatoprotective role of gold nanoparticles against murine hepatic schistosomiasis
description	Mohamed A Dkhil,1,2 Amira A Bauomy,2,3 Marwa SM Diab,4 Saleh Al-Quraishy21Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt; 3Department of Laboratory Sciences, College of Science & Arts, Qassim University, Buraydah, Saudi Arabia; 4Molecular Drug Evaluation Department, National Organization for Drug Control & Research (NODCAR), Giza, EgyptAbstract: In recent years, gold nanoparticles (AuNPs) have become the focus of much attention in biomedical research, especially in the context of nanomedicine, due to their distinctive physicochemical properties. The current study was planned to assess the effect of three dose levels of AuNPs on the gene expression, histology, and oxidative stress status of Schistosoma mansoni-infected mice liver. Inoculation of mice with 100 µL AuNPs at different doses (0.25, 0.5, and 1 mg/kg mice body weight) twice on day 46 and day 49 postinfection reduced the total worm burden, the egg load in the liver, and the granuloma size. AuNPs also appeared to decrease the activities of malondialdehyde and nitric oxide significantly, and increase the level of glutathione compared to the infected untreated group. Concomitantly, AuNPs ameliorated the inflammatory response by decreasing the mRNA expression of interleukin-1β, interleukin-6, tumor necrosis factor-α, interferon-γ, and inducible nitric oxide synthase. These consistent molecular, histopathological, and biochemical data suggest that AuNPs could ameliorate infection-induced damage in the livers of mice. Our results indicated that AuNPs are effective anti-schistosomal and antioxidant agents. Further confirmation of the role of nanogold as an anti-schistosomal agent, as well as its mechanism of action, requires further studies to be undertaken in the future.Keywords: nanogold, Schistosoma mansoni, liver, gene expressions, histopathology, oxidative stress, mice
format	article
author	Dkhil MA Bauomy AA Diab MSM Al-Quraishy S
author_facet	Dkhil MA Bauomy AA Diab MSM Al-Quraishy S
author_sort	Dkhil MA
title	Antioxidant and hepatoprotective role of gold nanoparticles against murine hepatic schistosomiasis
title_short	Antioxidant and hepatoprotective role of gold nanoparticles against murine hepatic schistosomiasis
title_full	Antioxidant and hepatoprotective role of gold nanoparticles against murine hepatic schistosomiasis
title_fullStr	Antioxidant and hepatoprotective role of gold nanoparticles against murine hepatic schistosomiasis
title_full_unstemmed	Antioxidant and hepatoprotective role of gold nanoparticles against murine hepatic schistosomiasis
title_sort	antioxidant and hepatoprotective role of gold nanoparticles against murine hepatic schistosomiasis
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/370bd3493e0c42be9c5e1d40cdd7cfcf
work_keys_str_mv	AT dkhilma antioxidantandhepatoprotectiveroleofgoldnanoparticlesagainstmurinehepaticschistosomiasis AT bauomyaa antioxidantandhepatoprotectiveroleofgoldnanoparticlesagainstmurinehepaticschistosomiasis AT diabmsm antioxidantandhepatoprotectiveroleofgoldnanoparticlesagainstmurinehepaticschistosomiasis AT alquraishys antioxidantandhepatoprotectiveroleofgoldnanoparticlesagainstmurinehepaticschistosomiasis
_version_	1718401976086036480

Antioxidant and hepatoprotective role of gold nanoparticles against murine hepatic schistosomiasis

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