Characterization and Cytotoxicity of Polyprenol Lipid and Vitamin E-TPGS Hybrid Nanoparticles for Betulinic Acid and Low-Substituted Hydroxyl Fullerenol in MHCC97H and L02 Cells

Ran Tao,1,2 Chengzhang Wang,1,2 Yin Lu,3 Changwei Zhang,1 Hao Zhou,1,2 Hongxia Chen,1 WenJun Li1 1Institute of Chemical Industry of Forest Products, CAF, Nanjing, Jiangsu Province 210042, People’s Republic of China; 2Research Institute of Forestry New Technology, CAF, Beijing 100091, Peopl...

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Autores principales:	Tao R, Wang C, Lu Y, Zhang C, Zhou H, Chen H, Li W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	polyprenol vitamin e-tpgs betulinic acid fullerenol anti-tumor Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3717e5278fb84d0f9b95ccdc33db882f
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spelling	oai:doaj.org-article:3717e5278fb84d0f9b95ccdc33db882f2021-12-02T08:54:58ZCharacterization and Cytotoxicity of Polyprenol Lipid and Vitamin E-TPGS Hybrid Nanoparticles for Betulinic Acid and Low-Substituted Hydroxyl Fullerenol in MHCC97H and L02 Cells1178-2013https://doaj.org/article/3717e5278fb84d0f9b95ccdc33db882f2020-04-01T00:00:00Zhttps://www.dovepress.com/characterization-and-cytotoxicity-of-polyprenol-lipid-and-vitamin-e-tp-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ran Tao,1,2 Chengzhang Wang,1,2 Yin Lu,3 Changwei Zhang,1 Hao Zhou,1,2 Hongxia Chen,1 WenJun Li1 1Institute of Chemical Industry of Forest Products, CAF, Nanjing, Jiangsu Province 210042, People’s Republic of China; 2Research Institute of Forestry New Technology, CAF, Beijing 100091, People’s Republic of China; 3General Hospital of Eastern Theater Command, Nanjing, Jiangsu Province 210002, People’s Republic of ChinaCorrespondence: Ran Tao; Chengzhang Wang Tel +86-025-85482421; +86-025-85482471Email trmoon1949@163.com; wangczlhs@sina.comBackground: This study demonstrated an innovative formulation including the polyprenol (GBP) lipid and vitamin E-TPGS hybrid nanoparticles (NPs) which was aimed to control the transfer of betulinic acid (BA) and low-substituted hydroxyl fullerenol (C60(OH)n). Additionally, it developed BA-C60(OH)n-GBP-TPGS-NPs delivery system and researched the anti-hepatocellular carcinoma (HCC) effects.Materials and Methods: The NPs were prepared by nanoprecipitation with ultrasonic-assisted emulsification (UAE) method. It was characterized by scanning electronic microscopy (SEM), transmission electron microscopy (TEM), FTIR spectrum, size distribution and zeta potential. Physical and chemical properties were evaluated through measurement of drug release, stability studies, drug loading efficiency (DE) and encapsulation efficiency (EE). Biological activities were evaluated through measurement of MTT assay, lactate dehydrogenase leakage assay (LDH), cell proliferation assays, cell apoptosis analysis, comet assay, wound healing assay, cell invasion and Western blot analysis.Results and Conclusions: The NPs exhibited clear distribution characteristics, improved solubility and stability. BA and C60(OH)n for the NPs displayed a biphasic release pattern with sustained drug release properties. The mixture of C60(OH)n with different hydroxyl groups may have a certain effect on the stability of the NPs system itself. The NPs could effectively inhibit MHCC97H cell proliferation, migration and invasion in vitro. Combined use of C60(OH)n and BA in GBP lipids may improve the inhibit effect of C60(OH)n or BA against HCC cells and reduce cytotoxicity and genotoxicity of C60(OH)n for normal cells. We concluded that one of the important mechanisms of BA-C60(OH)n-GBP-TPGS-NPs inhibiting MHCC97H cells is achieved by up-regulating the expression of Caspase-3, Caspase-8 and Caspase-9.Keywords: polyprenol, vitamin E-TPGS, betulinic acid, fullerenol, anti-tumorTao RWang CLu YZhang CZhou HChen HLi WDove Medical Pressarticlepolyprenolvitamin e-tpgsbetulinic acidfullerenolanti-tumorMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 2733-2749 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	polyprenol vitamin e-tpgs betulinic acid fullerenol anti-tumor Medicine (General) R5-920
spellingShingle	polyprenol vitamin e-tpgs betulinic acid fullerenol anti-tumor Medicine (General) R5-920 Tao R Wang C Lu Y Zhang C Zhou H Chen H Li W Characterization and Cytotoxicity of Polyprenol Lipid and Vitamin E-TPGS Hybrid Nanoparticles for Betulinic Acid and Low-Substituted Hydroxyl Fullerenol in MHCC97H and L02 Cells
description	Ran Tao,1,2 Chengzhang Wang,1,2 Yin Lu,3 Changwei Zhang,1 Hao Zhou,1,2 Hongxia Chen,1 WenJun Li1 1Institute of Chemical Industry of Forest Products, CAF, Nanjing, Jiangsu Province 210042, People’s Republic of China; 2Research Institute of Forestry New Technology, CAF, Beijing 100091, People’s Republic of China; 3General Hospital of Eastern Theater Command, Nanjing, Jiangsu Province 210002, People’s Republic of ChinaCorrespondence: Ran Tao; Chengzhang Wang Tel +86-025-85482421; +86-025-85482471Email trmoon1949@163.com; wangczlhs@sina.comBackground: This study demonstrated an innovative formulation including the polyprenol (GBP) lipid and vitamin E-TPGS hybrid nanoparticles (NPs) which was aimed to control the transfer of betulinic acid (BA) and low-substituted hydroxyl fullerenol (C60(OH)n). Additionally, it developed BA-C60(OH)n-GBP-TPGS-NPs delivery system and researched the anti-hepatocellular carcinoma (HCC) effects.Materials and Methods: The NPs were prepared by nanoprecipitation with ultrasonic-assisted emulsification (UAE) method. It was characterized by scanning electronic microscopy (SEM), transmission electron microscopy (TEM), FTIR spectrum, size distribution and zeta potential. Physical and chemical properties were evaluated through measurement of drug release, stability studies, drug loading efficiency (DE) and encapsulation efficiency (EE). Biological activities were evaluated through measurement of MTT assay, lactate dehydrogenase leakage assay (LDH), cell proliferation assays, cell apoptosis analysis, comet assay, wound healing assay, cell invasion and Western blot analysis.Results and Conclusions: The NPs exhibited clear distribution characteristics, improved solubility and stability. BA and C60(OH)n for the NPs displayed a biphasic release pattern with sustained drug release properties. The mixture of C60(OH)n with different hydroxyl groups may have a certain effect on the stability of the NPs system itself. The NPs could effectively inhibit MHCC97H cell proliferation, migration and invasion in vitro. Combined use of C60(OH)n and BA in GBP lipids may improve the inhibit effect of C60(OH)n or BA against HCC cells and reduce cytotoxicity and genotoxicity of C60(OH)n for normal cells. We concluded that one of the important mechanisms of BA-C60(OH)n-GBP-TPGS-NPs inhibiting MHCC97H cells is achieved by up-regulating the expression of Caspase-3, Caspase-8 and Caspase-9.Keywords: polyprenol, vitamin E-TPGS, betulinic acid, fullerenol, anti-tumor
format	article
author	Tao R Wang C Lu Y Zhang C Zhou H Chen H Li W
author_facet	Tao R Wang C Lu Y Zhang C Zhou H Chen H Li W
author_sort	Tao R
title	Characterization and Cytotoxicity of Polyprenol Lipid and Vitamin E-TPGS Hybrid Nanoparticles for Betulinic Acid and Low-Substituted Hydroxyl Fullerenol in MHCC97H and L02 Cells
title_short	Characterization and Cytotoxicity of Polyprenol Lipid and Vitamin E-TPGS Hybrid Nanoparticles for Betulinic Acid and Low-Substituted Hydroxyl Fullerenol in MHCC97H and L02 Cells
title_full	Characterization and Cytotoxicity of Polyprenol Lipid and Vitamin E-TPGS Hybrid Nanoparticles for Betulinic Acid and Low-Substituted Hydroxyl Fullerenol in MHCC97H and L02 Cells
title_fullStr	Characterization and Cytotoxicity of Polyprenol Lipid and Vitamin E-TPGS Hybrid Nanoparticles for Betulinic Acid and Low-Substituted Hydroxyl Fullerenol in MHCC97H and L02 Cells
title_full_unstemmed	Characterization and Cytotoxicity of Polyprenol Lipid and Vitamin E-TPGS Hybrid Nanoparticles for Betulinic Acid and Low-Substituted Hydroxyl Fullerenol in MHCC97H and L02 Cells
title_sort	characterization and cytotoxicity of polyprenol lipid and vitamin e-tpgs hybrid nanoparticles for betulinic acid and low-substituted hydroxyl fullerenol in mhcc97h and l02 cells
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/3717e5278fb84d0f9b95ccdc33db882f
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Characterization and Cytotoxicity of Polyprenol Lipid and Vitamin E-TPGS Hybrid Nanoparticles for Betulinic Acid and Low-Substituted Hydroxyl Fullerenol in MHCC97H and L02 Cells

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