Epicoccum nigrum P16, a sugarcane endophyte, produces antifungal compounds and induces root growth.

<h4>Background</h4>Sugarcane is one of the most important crops in Brazil, mainly because of its use in biofuel production. Recent studies have sought to determine the role of sugarcane endophytic microbial diversity in microorganism-plant interactions, and their biotechnological potenti...

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Autores principales:	Léia Cecilia de Lima Fávaro, Fernanda Luiza de Souza Sebastianes, Welington Luiz Araújo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/371edc2394aa4feca08016cfb2b196c9
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spelling	oai:doaj.org-article:371edc2394aa4feca08016cfb2b196c92021-11-18T07:16:32ZEpicoccum nigrum P16, a sugarcane endophyte, produces antifungal compounds and induces root growth.1932-620310.1371/journal.pone.0036826https://doaj.org/article/371edc2394aa4feca08016cfb2b196c92012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22675473/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Sugarcane is one of the most important crops in Brazil, mainly because of its use in biofuel production. Recent studies have sought to determine the role of sugarcane endophytic microbial diversity in microorganism-plant interactions, and their biotechnological potential. Epicoccum nigrum is an important sugarcane endophytic fungus that has been associated with the biological control of phytopathogens, and the production of secondary metabolites. In spite of several studies carried out to define the better conditions to use E. nigrum in different crops, little is known about the establishment of an endophytic interaction, and its potential effects on plant physiology.<h4>Methodology/principal findings</h4>We report an approach based on inoculation followed by re-isolation, molecular monitoring, microscopic analysis, plant growth responses to fungal colonization, and antimicrobial activity tests to study the basic aspects of the E. nigrum endophytic interaction with sugarcane, and the effects of colonization on plant physiology. The results indicate that E. nigrum was capable of increasing the root system biomass and producing compounds that inhibit the in vitro growth of sugarcane pathogens Fusarium verticillioides, Colletotrichum falcatum, Ceratocystis paradoxa, and Xanthomomas albilineans. In addition, E. nigrum preferentially colonizes the sugarcane surface and, occasionally, the endophytic environment.<h4>Conclusions/significance</h4>Our work demonstrates that E. nigrum has great potential for sugarcane crop application because it is capable of increasing the root system biomass and controlling pathogens. The study of the basic aspects of the interaction of E. nigrum with sugarcane demonstrated the facultative endophytism of E. nigrum and its preference for the phylloplane environment, which should be considered in future studies of biocontrol using this species. In addition, this work contributes to the knowledge of the interaction of this ubiquitous endophyte with the host plant, and also to a better use of microbial endophytes in agriculture.Léia Cecilia de Lima FávaroFernanda Luiza de Souza SebastianesWelington Luiz AraújoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e36826 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Léia Cecilia de Lima Fávaro Fernanda Luiza de Souza Sebastianes Welington Luiz Araújo Epicoccum nigrum P16, a sugarcane endophyte, produces antifungal compounds and induces root growth.
description	<h4>Background</h4>Sugarcane is one of the most important crops in Brazil, mainly because of its use in biofuel production. Recent studies have sought to determine the role of sugarcane endophytic microbial diversity in microorganism-plant interactions, and their biotechnological potential. Epicoccum nigrum is an important sugarcane endophytic fungus that has been associated with the biological control of phytopathogens, and the production of secondary metabolites. In spite of several studies carried out to define the better conditions to use E. nigrum in different crops, little is known about the establishment of an endophytic interaction, and its potential effects on plant physiology.<h4>Methodology/principal findings</h4>We report an approach based on inoculation followed by re-isolation, molecular monitoring, microscopic analysis, plant growth responses to fungal colonization, and antimicrobial activity tests to study the basic aspects of the E. nigrum endophytic interaction with sugarcane, and the effects of colonization on plant physiology. The results indicate that E. nigrum was capable of increasing the root system biomass and producing compounds that inhibit the in vitro growth of sugarcane pathogens Fusarium verticillioides, Colletotrichum falcatum, Ceratocystis paradoxa, and Xanthomomas albilineans. In addition, E. nigrum preferentially colonizes the sugarcane surface and, occasionally, the endophytic environment.<h4>Conclusions/significance</h4>Our work demonstrates that E. nigrum has great potential for sugarcane crop application because it is capable of increasing the root system biomass and controlling pathogens. The study of the basic aspects of the interaction of E. nigrum with sugarcane demonstrated the facultative endophytism of E. nigrum and its preference for the phylloplane environment, which should be considered in future studies of biocontrol using this species. In addition, this work contributes to the knowledge of the interaction of this ubiquitous endophyte with the host plant, and also to a better use of microbial endophytes in agriculture.
format	article
author	Léia Cecilia de Lima Fávaro Fernanda Luiza de Souza Sebastianes Welington Luiz Araújo
author_facet	Léia Cecilia de Lima Fávaro Fernanda Luiza de Souza Sebastianes Welington Luiz Araújo
author_sort	Léia Cecilia de Lima Fávaro
title	Epicoccum nigrum P16, a sugarcane endophyte, produces antifungal compounds and induces root growth.
title_short	Epicoccum nigrum P16, a sugarcane endophyte, produces antifungal compounds and induces root growth.
title_full	Epicoccum nigrum P16, a sugarcane endophyte, produces antifungal compounds and induces root growth.
title_fullStr	Epicoccum nigrum P16, a sugarcane endophyte, produces antifungal compounds and induces root growth.
title_full_unstemmed	Epicoccum nigrum P16, a sugarcane endophyte, produces antifungal compounds and induces root growth.
title_sort	epicoccum nigrum p16, a sugarcane endophyte, produces antifungal compounds and induces root growth.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/371edc2394aa4feca08016cfb2b196c9
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Epicoccum nigrum P16, a sugarcane endophyte, produces antifungal compounds and induces root growth.

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