Metabolic versatility and antibacterial metabolite biosynthesis are distinguishing genomic features of the fire blight antagonist Pantoea vagans C9-1.

<h4>Background</h4>Pantoea vagans is a commercialized biological control agent used against the pome fruit bacterial disease fire blight, caused by Erwinia amylovora. Compared to other biocontrol agents, relatively little is currently known regarding Pantoea genetics. Better understandin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Theo H M Smits, Fabio Rezzonico, Tim Kamber, Jochen Blom, Alexander Goesmann, Carol A Ishimaru, Jürg E Frey, Virginia O Stockwell, Brion Duffy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3723c8bdadaa456c8e25db5ab44ea313
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3723c8bdadaa456c8e25db5ab44ea313
record_format	dspace
spelling	oai:doaj.org-article:3723c8bdadaa456c8e25db5ab44ea3132021-11-18T06:50:10ZMetabolic versatility and antibacterial metabolite biosynthesis are distinguishing genomic features of the fire blight antagonist Pantoea vagans C9-1.1932-620310.1371/journal.pone.0022247https://doaj.org/article/3723c8bdadaa456c8e25db5ab44ea3132011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21789243/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Pantoea vagans is a commercialized biological control agent used against the pome fruit bacterial disease fire blight, caused by Erwinia amylovora. Compared to other biocontrol agents, relatively little is currently known regarding Pantoea genetics. Better understanding of antagonist mechanisms of action and ecological fitness is critical to improving efficacy.<h4>Principal findings</h4>Genome analysis indicated two major factors Contribute to biocontrol activity: competition for limiting substrates and antibacterial metabolite production. Pathways for utilization of a broad diversity of sugars and acquisition of iron were identified. Metabolism of sorbitol by P. vagans C9-1 may be a major metabolic feature in biocontrol of fire blight. Biosynthetic genes for the antibacterial peptide pantocin A were found on a chromosomal 28-kb genomic island, and for dapdiamide E on the plasmid pPag2. There was no evidence of potential virulence factors that could enable an animal or phytopathogenic lifestyle and no indication of any genetic-based biosafety risk in the antagonist.<h4>Conclusions</h4>Identifying key determinants contributing to disease suppression allows the development of procedures to follow their expression in planta and the genome sequence contributes to rationale risk assessment regarding the use of the biocontrol strain in agricultural systems.Theo H M SmitsFabio RezzonicoTim KamberJochen BlomAlexander GoesmannCarol A IshimaruJürg E FreyVirginia O StockwellBrion DuffyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e22247 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Theo H M Smits Fabio Rezzonico Tim Kamber Jochen Blom Alexander Goesmann Carol A Ishimaru Jürg E Frey Virginia O Stockwell Brion Duffy Metabolic versatility and antibacterial metabolite biosynthesis are distinguishing genomic features of the fire blight antagonist Pantoea vagans C9-1.
description	<h4>Background</h4>Pantoea vagans is a commercialized biological control agent used against the pome fruit bacterial disease fire blight, caused by Erwinia amylovora. Compared to other biocontrol agents, relatively little is currently known regarding Pantoea genetics. Better understanding of antagonist mechanisms of action and ecological fitness is critical to improving efficacy.<h4>Principal findings</h4>Genome analysis indicated two major factors Contribute to biocontrol activity: competition for limiting substrates and antibacterial metabolite production. Pathways for utilization of a broad diversity of sugars and acquisition of iron were identified. Metabolism of sorbitol by P. vagans C9-1 may be a major metabolic feature in biocontrol of fire blight. Biosynthetic genes for the antibacterial peptide pantocin A were found on a chromosomal 28-kb genomic island, and for dapdiamide E on the plasmid pPag2. There was no evidence of potential virulence factors that could enable an animal or phytopathogenic lifestyle and no indication of any genetic-based biosafety risk in the antagonist.<h4>Conclusions</h4>Identifying key determinants contributing to disease suppression allows the development of procedures to follow their expression in planta and the genome sequence contributes to rationale risk assessment regarding the use of the biocontrol strain in agricultural systems.
format	article
author	Theo H M Smits Fabio Rezzonico Tim Kamber Jochen Blom Alexander Goesmann Carol A Ishimaru Jürg E Frey Virginia O Stockwell Brion Duffy
author_facet	Theo H M Smits Fabio Rezzonico Tim Kamber Jochen Blom Alexander Goesmann Carol A Ishimaru Jürg E Frey Virginia O Stockwell Brion Duffy
author_sort	Theo H M Smits
title	Metabolic versatility and antibacterial metabolite biosynthesis are distinguishing genomic features of the fire blight antagonist Pantoea vagans C9-1.
title_short	Metabolic versatility and antibacterial metabolite biosynthesis are distinguishing genomic features of the fire blight antagonist Pantoea vagans C9-1.
title_full	Metabolic versatility and antibacterial metabolite biosynthesis are distinguishing genomic features of the fire blight antagonist Pantoea vagans C9-1.
title_fullStr	Metabolic versatility and antibacterial metabolite biosynthesis are distinguishing genomic features of the fire blight antagonist Pantoea vagans C9-1.
title_full_unstemmed	Metabolic versatility and antibacterial metabolite biosynthesis are distinguishing genomic features of the fire blight antagonist Pantoea vagans C9-1.
title_sort	metabolic versatility and antibacterial metabolite biosynthesis are distinguishing genomic features of the fire blight antagonist pantoea vagans c9-1.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/3723c8bdadaa456c8e25db5ab44ea313
work_keys_str_mv	AT theohmsmits metabolicversatilityandantibacterialmetabolitebiosynthesisaredistinguishinggenomicfeaturesofthefireblightantagonistpantoeavagansc91 AT fabiorezzonico metabolicversatilityandantibacterialmetabolitebiosynthesisaredistinguishinggenomicfeaturesofthefireblightantagonistpantoeavagansc91 AT timkamber metabolicversatilityandantibacterialmetabolitebiosynthesisaredistinguishinggenomicfeaturesofthefireblightantagonistpantoeavagansc91 AT jochenblom metabolicversatilityandantibacterialmetabolitebiosynthesisaredistinguishinggenomicfeaturesofthefireblightantagonistpantoeavagansc91 AT alexandergoesmann metabolicversatilityandantibacterialmetabolitebiosynthesisaredistinguishinggenomicfeaturesofthefireblightantagonistpantoeavagansc91 AT carolaishimaru metabolicversatilityandantibacterialmetabolitebiosynthesisaredistinguishinggenomicfeaturesofthefireblightantagonistpantoeavagansc91 AT jurgefrey metabolicversatilityandantibacterialmetabolitebiosynthesisaredistinguishinggenomicfeaturesofthefireblightantagonistpantoeavagansc91 AT virginiaostockwell metabolicversatilityandantibacterialmetabolitebiosynthesisaredistinguishinggenomicfeaturesofthefireblightantagonistpantoeavagansc91 AT brionduffy metabolicversatilityandantibacterialmetabolitebiosynthesisaredistinguishinggenomicfeaturesofthefireblightantagonistpantoeavagansc91
_version_	1718424284068577280

Metabolic versatility and antibacterial metabolite biosynthesis are distinguishing genomic features of the fire blight antagonist Pantoea vagans C9-1.

Ejemplares similares