Lactobacillus crispatus thrives in pregnancy hormonal milieu in a Nigerian patient cohort

Abstract Steroid hormones are one of the presumed modulators of Lactobacillus abundance in the vaginal epithelium. We set out to characterize the vaginal microbiome (VMB) and also provide an in-depth understanding of the relative contribution of estradiol (E2) and progesterone (P1) in shaping the va...

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Autores principales:	Nkechi Martina Odogwu, Chinedum Amara Onebunne, Jun Chen, Funmilola A. Ayeni, Marina R. S. Walther-Antonio, Oladapo O. Olayemi, Nicholas Chia, Akinyinka O. Omigbodun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/37288f1a63dd42a79b07d5806d587344
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spelling	oai:doaj.org-article:37288f1a63dd42a79b07d5806d5873442021-12-02T18:49:53ZLactobacillus crispatus thrives in pregnancy hormonal milieu in a Nigerian patient cohort10.1038/s41598-021-96339-y2045-2322https://doaj.org/article/37288f1a63dd42a79b07d5806d5873442021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96339-yhttps://doaj.org/toc/2045-2322Abstract Steroid hormones are one of the presumed modulators of Lactobacillus abundance in the vaginal epithelium. We set out to characterize the vaginal microbiome (VMB) and also provide an in-depth understanding of the relative contribution of estradiol (E2) and progesterone (P1) in shaping the vaginal microbiome of Nigerian women (n = 38) who experienced both uncomplicated term delivery and preterm delivery using samples longitudinally collected during pregnancy (17–21, 27–31, 36–41 weeks gestation) and 6 weeks postpartum. Vaginal swabs and blood samples were aseptically collected. Vaginal swabs were used for microbiome assessment using 16S ribosomal RNA (rRNA) gene sequencing. Blood samples were used for hormonal measurement using a competitive-based enzyme-linked immunosorbent assay (ELISA). Across several maternal covariates, maternal age, pregnancy status and delivery mode were not significantly associated with the vaginal microbiota whereas maternal E2 level (pE2 = 0.006, Omnibus), and P1 level (pP1 = 0.001, Omnibus) were significantly associated with the vaginal microbiome. E2 and P1 concentrations increased throughout pregnancy commensurately with increasing proportions of L. crispatus (pE2 = 0.036, pP1 = 0.034, Linear Mixed Model). An increasing trend of α-diversity was also observed as pregnancy progressed (pobserved ASV = 0.006, LMM). A compositional microbiome shift from Lactobacillus profile to non-Lactobacillus profile was observed in most postnatal women (pCST IV < 0.001, LMM). Analysis of our data shows a species-specific link between pregnancy steroid hormone concentration and L. crispatus abundance.Nkechi Martina OdogwuChinedum Amara OnebunneJun ChenFunmilola A. AyeniMarina R. S. Walther-AntonioOladapo O. OlayemiNicholas ChiaAkinyinka O. OmigbodunNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nkechi Martina Odogwu Chinedum Amara Onebunne Jun Chen Funmilola A. Ayeni Marina R. S. Walther-Antonio Oladapo O. Olayemi Nicholas Chia Akinyinka O. Omigbodun Lactobacillus crispatus thrives in pregnancy hormonal milieu in a Nigerian patient cohort
description	Abstract Steroid hormones are one of the presumed modulators of Lactobacillus abundance in the vaginal epithelium. We set out to characterize the vaginal microbiome (VMB) and also provide an in-depth understanding of the relative contribution of estradiol (E2) and progesterone (P1) in shaping the vaginal microbiome of Nigerian women (n = 38) who experienced both uncomplicated term delivery and preterm delivery using samples longitudinally collected during pregnancy (17–21, 27–31, 36–41 weeks gestation) and 6 weeks postpartum. Vaginal swabs and blood samples were aseptically collected. Vaginal swabs were used for microbiome assessment using 16S ribosomal RNA (rRNA) gene sequencing. Blood samples were used for hormonal measurement using a competitive-based enzyme-linked immunosorbent assay (ELISA). Across several maternal covariates, maternal age, pregnancy status and delivery mode were not significantly associated with the vaginal microbiota whereas maternal E2 level (pE2 = 0.006, Omnibus), and P1 level (pP1 = 0.001, Omnibus) were significantly associated with the vaginal microbiome. E2 and P1 concentrations increased throughout pregnancy commensurately with increasing proportions of L. crispatus (pE2 = 0.036, pP1 = 0.034, Linear Mixed Model). An increasing trend of α-diversity was also observed as pregnancy progressed (pobserved ASV = 0.006, LMM). A compositional microbiome shift from Lactobacillus profile to non-Lactobacillus profile was observed in most postnatal women (pCST IV < 0.001, LMM). Analysis of our data shows a species-specific link between pregnancy steroid hormone concentration and L. crispatus abundance.
format	article
author	Nkechi Martina Odogwu Chinedum Amara Onebunne Jun Chen Funmilola A. Ayeni Marina R. S. Walther-Antonio Oladapo O. Olayemi Nicholas Chia Akinyinka O. Omigbodun
author_facet	Nkechi Martina Odogwu Chinedum Amara Onebunne Jun Chen Funmilola A. Ayeni Marina R. S. Walther-Antonio Oladapo O. Olayemi Nicholas Chia Akinyinka O. Omigbodun
author_sort	Nkechi Martina Odogwu
title	Lactobacillus crispatus thrives in pregnancy hormonal milieu in a Nigerian patient cohort
title_short	Lactobacillus crispatus thrives in pregnancy hormonal milieu in a Nigerian patient cohort
title_full	Lactobacillus crispatus thrives in pregnancy hormonal milieu in a Nigerian patient cohort
title_fullStr	Lactobacillus crispatus thrives in pregnancy hormonal milieu in a Nigerian patient cohort
title_full_unstemmed	Lactobacillus crispatus thrives in pregnancy hormonal milieu in a Nigerian patient cohort
title_sort	lactobacillus crispatus thrives in pregnancy hormonal milieu in a nigerian patient cohort
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/37288f1a63dd42a79b07d5806d587344
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Lactobacillus crispatus thrives in pregnancy hormonal milieu in a Nigerian patient cohort

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