Dual functionality nanobioconjugates targeting intracellular bacteria in cancer cells with enhanced antimicrobial activity

Abstract Bacterial drug resistance has emerged as a serious global threat mandating the development of novel methodologies that allow facile modulation of antimicrobial action in a controlled fashion. Conjugating antibiotics to nanoparticles helps to meet this goal by increasing the drug’s overall a...

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Autores principales:	Rohini Singh, Smita Patil, Neetu Singh, Shalini Gupta
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3731b35f52ed4b44bbd66e2e500ec66b
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spelling	oai:doaj.org-article:3731b35f52ed4b44bbd66e2e500ec66b2021-12-02T15:05:07ZDual functionality nanobioconjugates targeting intracellular bacteria in cancer cells with enhanced antimicrobial activity10.1038/s41598-017-06014-42045-2322https://doaj.org/article/3731b35f52ed4b44bbd66e2e500ec66b2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06014-4https://doaj.org/toc/2045-2322Abstract Bacterial drug resistance has emerged as a serious global threat mandating the development of novel methodologies that allow facile modulation of antimicrobial action in a controlled fashion. Conjugating antibiotics to nanoparticles helps to meet this goal by increasing the drug’s overall avidity, bioavailability and easier internalisation into mammalian cells, targeting bacteria that otherwise escape antibacterial action by host cell-localisation. We used polymyxin B sulfate (PMB) and sushi peptide as model drugs against Gram-negative bacteria and established their enhanced antimicrobial activity on Escherichia coli (E. coli) cells after conjugation to gold nanoparticles (AuNPs). The efficacy of the bioconjugates was also tested on Salmonella typhi (S. typhi) bacteria infected into cervical cancer cells (HeLa) and further improved through specific targeting via folate receptors. Our results demonstrate significantly lower inhibitory concentration values for sushi-NP assemblies as compared to free drug, especially at optimal drug loading levels. No major cytotoxicity was observed in mammalian cells alone.Rohini SinghSmita PatilNeetu SinghShalini GuptaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rohini Singh Smita Patil Neetu Singh Shalini Gupta Dual functionality nanobioconjugates targeting intracellular bacteria in cancer cells with enhanced antimicrobial activity
description	Abstract Bacterial drug resistance has emerged as a serious global threat mandating the development of novel methodologies that allow facile modulation of antimicrobial action in a controlled fashion. Conjugating antibiotics to nanoparticles helps to meet this goal by increasing the drug’s overall avidity, bioavailability and easier internalisation into mammalian cells, targeting bacteria that otherwise escape antibacterial action by host cell-localisation. We used polymyxin B sulfate (PMB) and sushi peptide as model drugs against Gram-negative bacteria and established their enhanced antimicrobial activity on Escherichia coli (E. coli) cells after conjugation to gold nanoparticles (AuNPs). The efficacy of the bioconjugates was also tested on Salmonella typhi (S. typhi) bacteria infected into cervical cancer cells (HeLa) and further improved through specific targeting via folate receptors. Our results demonstrate significantly lower inhibitory concentration values for sushi-NP assemblies as compared to free drug, especially at optimal drug loading levels. No major cytotoxicity was observed in mammalian cells alone.
format	article
author	Rohini Singh Smita Patil Neetu Singh Shalini Gupta
author_facet	Rohini Singh Smita Patil Neetu Singh Shalini Gupta
author_sort	Rohini Singh
title	Dual functionality nanobioconjugates targeting intracellular bacteria in cancer cells with enhanced antimicrobial activity
title_short	Dual functionality nanobioconjugates targeting intracellular bacteria in cancer cells with enhanced antimicrobial activity
title_full	Dual functionality nanobioconjugates targeting intracellular bacteria in cancer cells with enhanced antimicrobial activity
title_fullStr	Dual functionality nanobioconjugates targeting intracellular bacteria in cancer cells with enhanced antimicrobial activity
title_full_unstemmed	Dual functionality nanobioconjugates targeting intracellular bacteria in cancer cells with enhanced antimicrobial activity
title_sort	dual functionality nanobioconjugates targeting intracellular bacteria in cancer cells with enhanced antimicrobial activity
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3731b35f52ed4b44bbd66e2e500ec66b
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Dual functionality nanobioconjugates targeting intracellular bacteria in cancer cells with enhanced antimicrobial activity

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