A prospective study revealing the role of an immune-related eRNA, WAKMAR2, in breast cancer

Abstract Enhancer RNAs (eRNAs) are a subclass of non-coding RNAs that are generated during the transcription of enhancer regions and play an important role in tumourigenesis. In this study, we focused on the crucial eRNAs that participate in immune responses in invasive breast cancer (IBC). We first...

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Autores principales: Linbang Wang, Jingkun Liu, Jiaojiao Tai, Nian Zhou, Tianji Huang, Yuzhou Xue, Zhengxue Quan
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Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/374478e299364993abc577d76e812d5d
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spelling oai:doaj.org-article:374478e299364993abc577d76e812d5d2021-12-02T16:06:43ZA prospective study revealing the role of an immune-related eRNA, WAKMAR2, in breast cancer10.1038/s41598-021-94784-32045-2322https://doaj.org/article/374478e299364993abc577d76e812d5d2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94784-3https://doaj.org/toc/2045-2322Abstract Enhancer RNAs (eRNAs) are a subclass of non-coding RNAs that are generated during the transcription of enhancer regions and play an important role in tumourigenesis. In this study, we focused on the crucial eRNAs that participate in immune responses in invasive breast cancer (IBC). We first used The Cancer Genome Atlas and Human enhancer RNA Atlas to screen for tissue-specific eRNAs and their target genes. Through Pearson correlation analysis with immune genes, the eRNA WAKMAR2 was identified as a key candidate involved in IBC. Our further research suggested that WAKMAR2 is crucial in regulating the tumour microenvironment and may function by regulating immune-related genes, including IL27RA, RAC2, FABP7, IGLV1-51, IGHA1, and IGHD. Quantitative reverse transcription-polymerase chain reaction was used to detect the expression of WAKMAR2 in IBC and normal tissues, and the effect of WAKMAR2 on the regulation of downstream genes in MB-231 and MCF7 cells was studied in vitro. WAKMAR2 was found to be highly involved in tumour immunity and was downregulated in IBC tissues. Furthermore, the expression of WAKMAR2 and its target genes was observed at the pan-cancer level. This study provides evidence to suggest new potential targets for the treatment of breast cancer.Linbang WangJingkun LiuJiaojiao TaiNian ZhouTianji HuangYuzhou XueZhengxue QuanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Linbang Wang
Jingkun Liu
Jiaojiao Tai
Nian Zhou
Tianji Huang
Yuzhou Xue
Zhengxue Quan
A prospective study revealing the role of an immune-related eRNA, WAKMAR2, in breast cancer
description Abstract Enhancer RNAs (eRNAs) are a subclass of non-coding RNAs that are generated during the transcription of enhancer regions and play an important role in tumourigenesis. In this study, we focused on the crucial eRNAs that participate in immune responses in invasive breast cancer (IBC). We first used The Cancer Genome Atlas and Human enhancer RNA Atlas to screen for tissue-specific eRNAs and their target genes. Through Pearson correlation analysis with immune genes, the eRNA WAKMAR2 was identified as a key candidate involved in IBC. Our further research suggested that WAKMAR2 is crucial in regulating the tumour microenvironment and may function by regulating immune-related genes, including IL27RA, RAC2, FABP7, IGLV1-51, IGHA1, and IGHD. Quantitative reverse transcription-polymerase chain reaction was used to detect the expression of WAKMAR2 in IBC and normal tissues, and the effect of WAKMAR2 on the regulation of downstream genes in MB-231 and MCF7 cells was studied in vitro. WAKMAR2 was found to be highly involved in tumour immunity and was downregulated in IBC tissues. Furthermore, the expression of WAKMAR2 and its target genes was observed at the pan-cancer level. This study provides evidence to suggest new potential targets for the treatment of breast cancer.
format article
author Linbang Wang
Jingkun Liu
Jiaojiao Tai
Nian Zhou
Tianji Huang
Yuzhou Xue
Zhengxue Quan
author_facet Linbang Wang
Jingkun Liu
Jiaojiao Tai
Nian Zhou
Tianji Huang
Yuzhou Xue
Zhengxue Quan
author_sort Linbang Wang
title A prospective study revealing the role of an immune-related eRNA, WAKMAR2, in breast cancer
title_short A prospective study revealing the role of an immune-related eRNA, WAKMAR2, in breast cancer
title_full A prospective study revealing the role of an immune-related eRNA, WAKMAR2, in breast cancer
title_fullStr A prospective study revealing the role of an immune-related eRNA, WAKMAR2, in breast cancer
title_full_unstemmed A prospective study revealing the role of an immune-related eRNA, WAKMAR2, in breast cancer
title_sort prospective study revealing the role of an immune-related erna, wakmar2, in breast cancer
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/374478e299364993abc577d76e812d5d
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